Machinery Dynamics
Mostrando 25-36 de 54 artigos, teses e dissertações.
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25. Site-specific incorporation of biophysical probes into proteins.
Biophysical probes which can detect structural changes in proteins and the interaction of proteins with other macromolecules are important tools in studying protein function. Many difficulties remain, however, in introducing probes into proteins site-specifically. Here we report the successful site-specific incorporation of a spin-labeled, a fluorescent, and
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26. Designed transcription factors as structural, functional and therapeutic probes of chromatin in vivo: Fourth in review series on chromatin dynamics
Despite its central importance in gene regulation, chromatin in mammalian cells remains relatively poorly understood—a predicament due to the paucity of robust genetic tools in mammals, the complexity of the chromatin remodeling machinery, and the dynamic properties of chromatin in vivo. Here we review recent developments in understanding endogenous mammal
Oxford University Press.
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27. Characterization of Protein and Transcript Levels of the Chaperonin Containing Tailless Complex Protein-1 and Tubulin during Light-Regulated Growth of Oat Seedlings1
In grass seedlings the network of cortical microtubules is reorganized during light-dependent growth of coleoptiles and mesocotyls. We investigated the effects of light-dependent growth on the relative steady-state levels of the mRNAs and protein levels of α-tubulin and the ε-subunit of the chaperonin containing tailless complex protein-1 in oat (Avena sat
American Society of Plant Physiologists.
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28. The application of statistical physics to evolutionary biology
A number of fundamental mathematical models of the evolutionary process exhibit dynamics that can be difficult to understand analytically. Here we show that a precise mathematical analogy can be drawn between certain evolutionary and thermodynamic systems, allowing application of the powerful machinery of statistical physics to analysis of a family of evolut
National Academy of Sciences.
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29. Use of Fluorescent Protein Tags to Study Nuclear Organization of the Spliceosomal Machinery in Transiently Transformed Living Plant CellsD⃞
Although early studies suggested that little compartmentalization exists within the nucleus, more recent studies on metazoan systems have identified a still increasing number of specific subnuclear compartments. Some of these compartments are dynamic structures; indeed, protein and RNA-protein components can cycle between different domains. This is particula
The American Society for Cell Biology.
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30. Annexin II regulates multivesicular endosome biogenesis in the degradation pathway of animal cells
Proteins of the annexin family are believed to be involved in membrane-related processes, but their precise functions remain unclear. Here, we have made use of several experimental approaches, including pathological conditions, RNA interference and in vitro transport assays, to study the function of annexin II in the endocytic pathway. We find that annexin�
Oxford University Press.
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31. Syntaxin 17 Is Abundant in Steroidogenic Cells and Implicated in Smooth Endoplasmic Reticulum Membrane Dynamics
The endoplasmic reticulum (ER) consists of subcompartments that have distinct protein constituents, morphological appearances, and functions. To understand the mechanisms that regulate the intricate and dynamic organization of the endoplasmic reticulum, it is important to identify and characterize the molecular machinery involved in the assembly and mai
The American Society for Cell Biology.
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32. The dynamics of coiled bodies in the nucleus of adenovirus-infected cells.
The coiled body is a specific intranuclear structure of unknown function that is enriched in splicing small nuclear ribonucleoproteins (snRNPs). Because adenoviruses make use of the host cell-splicing machinery and subvert the normal subnuclear organization, we initially decided to investigate the effect of adenovirus infection on the coiled body. The result
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33. Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane
Selective protein export from the endoplasmic reticulum is mediated by COPII vesicles. Here, we investigated the dynamics of fluorescently labelled cargo and non-cargo proteins during COPII vesicle formation using single-molecule microscopy combined with an artificial planar lipid bilayer. Single-molecule analysis showed that the Sar1p–Sec23/24p-cargo comp
Nature Publishing Group.
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34. Mitotic Chromosome Condensation Requires Brn1p, the Yeast Homologue of Barren
In vitro studies suggest that the Barren protein may function as an activator of DNA topoisomerase II and/or as a component of the Xenopus condensin complex. To better understand the role of Barren in vivo, we generated conditional alleles of the structural gene for Barren (BRN1) in Saccharomyces cerevisiae. We show that Barren is an essential protein requir
The American Society for Cell Biology.
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35. Focal adhesions as mechanosensors: A physical mechanism
Focal adhesions (FA) are large, multiprotein complexes that provide a mechanical link between the cytoskeletal contractile machinery and the extracellular matrix. FA exhibit mechanosensitive properties; they self-assemble and elongate upon application of pulling forces and dissociate when these forces are decreased. We propose a thermodynamic model for the m
National Academy of Sciences.
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36. Manipulating the mammalian genome by homologous recombination
Gene targeting in mammalian cells has proven invaluable in biotechnology, in studies of gene structure and function, and in understanding chromosome dynamics. It also offers a potential tool for gene-therapeutic applications. Two limitations constrain the current technology: the low rate of homologous recombination in mammalian cells and the high rate of ran
The National Academy of Sciences.