Lysis Centrifugation
Mostrando 1-12 de 120 artigos, teses e dissertações.
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1. Histoplasma capsulatum fungemia in patients with acquired immunodeficiency syndrome : detection by lysis-centrifugation blood-culturing technique
Histoplasmose progressiva disseminada (HPD) tem aumentado e é causa comum de infecção em pacientes com síndrome da imunodeficiência adquirida (Aids). Relatamos 21 casos de HPD associado com Aids diagnosticada pela técnica de hemocultivo por lise-centrifugação. Os achados clínicos mais prevalentes foram febre, perda de peso, sintomas respiratórios e
Publicado em: 2010
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2. Bloodstream infections in late-stage acquired immunodeficiency syndrome patients evaluated by a lysis centrifugation system
Opportunistic infections, which affect acquired immunodeficiency syndrome (Aids) patients, are frequently disseminated and may cause bloodstream infections (BSI). The aim of this study was to evaluate the main causes of BSI in Aids patients with advanced stage of the disease, with special emphasis on the identification of fungemia. During a 21 months period,
Memórias do Instituto Oswaldo Cruz. Publicado em: 2003-06
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3. Comparison of lysis-centrifugation with lysis-filtration and a conventional unvented bottle for blood cultures.
Evaluation of a commercially available lysis-centrifugation blood culture system (Isolator, DuPont Co., Wilmington, Del.) and a lysis-filtration blood culture system for 3,111 cultures showed that both methods had comparable recoveries (73 and 68%, respectively) of significant aerobic and facultatively anaerobic isolates. The unvented conventional blood cult
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4. Isolation of Mycobacterium chelonei with the lysis-centrifugation blood culture technique.
Mycobacterium chelonei was isolated from a patient by the lysis-centrifugation and the conventional two-bottle blood culture methods. The lysis-centrifugation method was significantly more sensitive and rapid than the conventional method in detecting and isolating this organism; quantitations done by this method were useful for monitoring response to therapy
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5. Clinical evaluation of the lysis-centrifugation blood culture system for the detection of fungemia and comparison with a conventional biphasic broth blood culture system.
In a comparative fungal blood culture study, a lysis-centrifugation system (Isolator; Du Pont Co., Wilmington, Del.) detected 89% of all episodes of fungemia; the lysis-centrifugation system detected fungemia exclusively or significantly earlier than did a biphasic brain heart infusion bottle system 83% of the time. The lysis-centrifugation system was partic
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6. Effect of storage of the du Pont lysis-centrifugation system on recovery of bacteria and fungi in a prospective clinical trial.
A commercially available lysis-centrifugation system was compared with a conventional biphasic brain heart infusion medium in a prospective clinical study of 5,125 fungal blood cultures. Recovery rates were compared between two time periods to assess the effect of 25 degrees C storage before processing by the lysis-centrifugation system. The lysis-centrifuga
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7. Evaluation of Conventional Castaneda and Lysis Centrifugation Blood Culture Techniques for Diagnosis of Human Brucellosis
We investigated the role of the lysis centrifugation blood culture technique over the conventional Castaneda technique for the diagnosis of human brucellosis. The lysis centrifugation technique has been found to be more sensitive in both acute (20% higher sensitivity; P < 0.00001) and chronic (40% higher sensitivity; P = 0.087) forms of brucellosis. The majo
American Society for Microbiology.
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8. Blood culture bottles are superior to lysis-centrifugation tubes for bacteriological diagnosis of spontaneous bacterial peritonitis.
The conventional method of ascitic fluid culturing was compared with the bedside inoculation of ascites into blood culture bottles and into lysis-centrifugation tubes. The conventional culture method was compared with the blood culture bottle method in 31 episodes of spontaneous bacterial peritonitis (SBP). Cultures were positive with the conventional cultur
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9. Mycobacterium tuberculosis bacteremia detected by the Isolator lysis-centrifugation blood culture system.
Mycobacterium tuberculosis was detected by the Isolator lysis-centrifugation blood culture system from the blood of a patient with tuberculosis of the breast. The organism also grew on conventional laboratory media inoculated with pleural fluid from the patient.
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10. Inhibition of pneumococcal autolysis in lysis-centrifugation blood culture.
The recovery of Streptococcus pneumoniae from the Isolator lysis-centrifugation blood culture has been low in many studies. The poor survival of pneumococci was not due to toxicity of the Isolator medium but to autolysis before plating. This autolysis was completely inhibited by adding 10 mM phosphorylcholine to the Isolator medium.
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11. Comparison of the lysis-centrifugation and agitated biphasic blood culture systems for detection of fungemia.
Although the detection of fungemia has been improved by the use of vented or biphasic blood culture bottles, the best recovery and earliest detection have been reported in the Isolator lysis-centrifugation system. It was recently demonstrated that improved detection of both bacteria and fungi was accomplished by mechanically agitating blood culture bottles f
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12. Reproducibility of lysis-centrifugation cultures for quantification of Mycobacterium avium complex bacteremia.
While quantitative mycobacterial blood cultures have been accepted as the standard for evaluating response to various Mycobacterium avium complex (MAC) treatment regimens, variability in this methodology has not been evaluated in a rigorous fashion. We thus studied the reproducibility of quantitative MAC cultures by a lysis-centrifugation culture system with