Lymphocytotoxicity
Mostrando 1-12 de 19 artigos, teses e dissertações.
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1. Avaliação de diferentes metodologias laboratoriais para detecção de aloanticorpos plaquetários. Determinação da prevalência e importância clínica destes aloanticorpos em pacientes transfundidos / Evaluation of different methodologies for platelet alloantibodies detection. Clinical impact and prevalence of platelet antibodies in transfused patients.
A aloimunização contra antígenos plaquetários é um dos grandes desafios no tratamento de pacientes refratários à transfusão de plaquetas. OBJETIVOS: avaliar o desempenho de metodologias mais utilizadas em laboratórios de referência internacional para a pesquisa de anticorpos plaquetários (PAP); verificar a prevalência destes anticorpos na nossa p
Publicado em: 2008
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2. Use of cryopreserved virus-infected target cells in a lymphocytotoxicity 51Cr release microassay for cell-mediated immunity to cytomegalovirus.
A new 51Cr-release microassay is described for the measurement of cell-mediated immunity to cytomegalovirus, using cryopreserved target cells acutely infected with cytomegalovirus.
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3. Lymphocyte reactivity of workers exposed to carcinogenic and non-carcinogenic chemicals.
Immunological studies have shown an increased lymphocyte reactivity in patients with early stage bladder cancer and individuals with pre-stage T1 exposed to bladder carcinogens (2-naphthylamine and industrial 1-naphthylamine containing 4-8% 2-naphthylamine) before 1952-that is, those at high risk of developing bladder cancer. Because of the close chemical si
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4. Brain-reactive lymphocytotoxic antibodies in the serum of patients with systemic lupus erythematosus.
Homogenized tissue from the frontal cortex of normal human brains obtained at postmortem examination was used to absorb lymphocytotoxic antibody from the serum of six patients with systemic lupus erythematosus (SLE). Four absorptions of all of the SLE sera with equal volumes of homogenized brain tissue at 4 degrees C depleted their cytotoxic capacity more th
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5. Mycoplasma-dependent activation of normal mouse lymphocytes: requirement for functional T lymphocytes in the cytotoxicity reaction mediated by Mycoplasma arthritidis.
Syngeneic and allogeneic target cells were killed in the presence of CBA mouse lymphocytes and viable Mycoplasma arthritidis. Medium supplementation had no effect on the response. Nonviable M. arthritidis was also capable of stimulating lymphocytotoxicity, although to a much lesser extent. Cytotoxicity was shown to be largely dependent upon the lymphocytes,
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6. Induction of feline immunodeficiency virus-specific cytotoxic T cells in vivo with carrier-free synthetic peptide.
The role of cellular immunity in the establishment and progression of immunosuppressive lentivirus infection remains equivocal. To develop a model system with which these aspects of the host immune response can be studied experimentally, we examined the response of cats to a hybrid peptide containing predicted T-and B-cell epitopes from the gag and env genes
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7. Human T-cell heterogeneity as delineated with a specific human thymus lymphocyte antiserum. In vitro effects on mitogen response mixed leukocyte culture, cell-mediated lymphocytotoxicity, and lymphokine production.
Human peripheral blood lymphocytes (PBL) were evaluated by their responses to phytohemmagglutinin (PHA-P), concanavallin A (con-A), and pokeweed mitogen (PWM), both before and after treatment with an antiserum against human thymic lymphocyte antigens (HTLA) that had been made T-cell-specific by multiple absorptions with immunoglobulin EAC-positive lymphoblas
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8. Utilization of cultured human lymphoid cells for detection of humoral sensitization in prospective recipients of kidney transplants.
Prospective recipients of kidney transplants were tested for lymphocytotoxicity; from these we selected 102 sera that lacked cytotoxic antibodies against peripheral lymphocytes from at least 80 unrelated subjects. To detect humoral sensitization, we then reacted these with 17 cultured human lymphoid cell lines having different HL-A phenotypes. Cytotoxic anti
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9. Impaired Cellular Immunity to Rubella Virus in Congenital Rubella
Specific cell-mediated immunity (CMI) responses to rubella virus were studied in 12 children with documented congenital rubella syndrome employing a 51Cr lymphocytotoxicity microassay. Hemagglutination inhibition antibody was detected in 11 of the 12 children, with titers ranging from 1:4 to 1:128. CMI to rubella virus was demonstrated in only 3 of the 11 an
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10. Correction of adenosine deaminase deficiency in cultured human T and B cells by retrovirus-mediated gene transfer.
A retroviral vector called SAX, containing the cloned human cDNA for adenosine deaminase (ADA), has been constructed and used to introduce the ADA gene into cultured T- and B-lymphocyte lines derived from patients with ADA deficiency. DNA analysis showed that the SAX vector was inserted intact into the T and B cells at approximately one copy per cell. The tr
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11. Persistence of HLA-B27 cross-reactive bacteria in bowel flora of patients with ankylosing spondylitis.
Previous studies have shown that antisera raised in rabbits to certain enteric bacteria (cross-reactive bacteria) are capable of specifically lysing in a 51chromium-release lymphocytotoxicity test the lymphocytes of HLA-B27-positive (B27+) patients with ankylosing spondylitis (AS). This study investigated the clinical relevance of this finding by ascertainin
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12. Inhibition of humoral and cell-mediated immune responses in man by distinct suppressor cell systems.
Studies were designed to investigate whether the suppressor cell systems that regulate the humoral and cell-mediated immune responses belong to the same subsets of T cells or different subsets. Mitogen-activated suppressor cells were simultaneously assayed for their ability to inhibit (a) pokeweed mitogen-induced generation of plasma cells, (b) blastogenic r