Llc Mk2 Cells
Mostrando 1-12 de 71 artigos, teses e dissertações.
-
1. Antidiarrheal activity of extracts from Maytenus gonoclada and inhibition of Dengue virus by lupeol
ABSTRACT Diarrhea is an infectious disease caused by bacterial, virus, or protozoan, and dengue is caused by virus, included among the neglected diseases in several underdeveloped and developing countries, with an urgent demand for new drugs. Considering the antidiarrheal potential of species of Maytenus genus, a phytochemical investigation followed by antib
An. Acad. Bras. Ciênc.. Publicado em: 2017-09
-
2. Mecanismos moleculares da ação tóxica pró-oxidante de 1,4-diamino-2-butanona, um análogo de putrescina, sobre células de mamíferos e Trypanosoma cruzi / The Molecular mechanisms of pro-oxidant activity of 1,4-diamino-2-butanone, a putrescine analogue, to mammalian cells and Trypanosoma cruzi
α-Aminocarbonyl componds such as 5-aminolevunilic acid (ALA) and aminoacetone (AA) have been shown to exhibit pro-oxidant properties. These compounds undergo phosphate-catalyzed enolization in physiological pH and subsequent aerobic oxidation, yielding reactive oxygen species, NH4+ ions and an α-oxoaldehyde highly cytotoxic. The α-aminoketone 1
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 22/06/2012
-
3. Trypanosoma cruzi interaction with the extra-cellular matrix: modeling the TC85-11 protein and mapping the laminin-binding site / Trypanosoma cruzi e a interação com a matriz extracelular: modelagem da proteína Tc85-11 e determinação do sítio de ligação a laminina
Trypanosoma cruzi expresses the Tc85 proteins, a set of surface glycoproteins belonging to the gp85/trans-sialidase supergene family. In this report we show a structure model for Tc85-11 a member of this family, which has adhesive properties to laminin and to the host cell surfaces. That structure consists in an N-terminus β-propeller and a C-terminus &
Publicado em: 2003
-
4. Replication of Dengue Virus Type 2 in Aedes albopictus Cell Culture
The replication of type 2 dengue (D-2) virus in Aedes albopictus (Aal) mosquito cell cultures differed from that in vertebrate (LLC-MK2) rhesus monkey kidney cells. Virus readily replicated in Aal cells at either 30 or 37 C, but had no apparent effect on the host cell. Persistent infection was established with continual virus production for at least 6 months
-
5. Characteristics of Serially Propagated Monkey Kidney Cell Cultures with Persistent Rubella Infection
Maassab, H. F. (University of Michigan, Ann Arbor), and J. A. Veronelli. Characteristics of serially propagated monkey kidney cell cultures with persistent rubella infection. J. Bacteriol. 91:436–441. 1966.—A persistent infection of LLC-MK2 cells with rubella virus has been established and maintained for over 3 years. This “carrier culture,” designat
-
6. Comparison of Three Methods Used to Isolate Dengue Virus Type 2
During the 1969 dengue epidemic in Puerto Rico, human sera and Aedes aegypti mosquitoes were collected for virus isolation and identification. Three methods of isolation were used and compared. In the first method, we inoculated newborn mice by the intracranial route, noted any signs of illness, and serially passed specimens in mice until virus was isolated.
-
7. Application of Freeze-Etching Method to the Study of Reovirus-Infected LLC-MK2 Cells
A continuous cell line of rhesus monkey kidney cells, LLC-MK2, was infected with reovirus type 1 (Lang). The cells were freeze-etched as a method for observing the structural details of the reovirus-induced cytoplasmic inclusion. Information on maturation may be obtained by preparing infected cells for electron microscopy by freeze-etching.
-
8. Simplified plaque reduction neutralization assay for dengue viruses by semimicro methods in BHK-21 cells: comparison of the BHK suspension test with standard plaque reduction neutralization.
A newly modified semimicro plaque reduction neutralization test (PRNT) in BHK cells was compared with a standard PRNT in bottles with LLC-MK2 monolayers and with an LLC-MK2 PRNT adapted to semimicro methods. The BHK semimicro PRNT compared favorably in terms of sensitivity in detecting dengue antibody (96%), specificity at a screening dilution (95%), and abi
-
9. Dengue Virus Plaque Development in Simian Cell Systems: II. Agar Variables and Effect of Chemical Additives
Dengue type 2 virus, strain New Guinea B, plaqued with equal facility and titer under overlays containing six different grades of commercial agar in the LLC-MK2 cell system. Doubling the agar volume on LLC-MK2 cell monolayers increased the plaque development time of dengue type 1, strain Hawaii. Storage of agar at 56 C reduced or totally abolished dengue typ
-
10. Fluorescent-Antibody Marker for Vaccine-Induced Rubella Antibodies
Tests with LLC-MK2 and BHK-21 cells showed that the fluorescent-antibody (FA) procedure can be used to differentiate antibodies resulting from natural infection and antibodies stimulated by rubella vaccine virus. Negative or weak FA reactions in LLC-MK2 cells and strong reactions in BHK-21 cells indicated antibody stimulated by the vaccine virus; strong reac
-
11. Virus Susceptibility of a New Simian Cell Line of Fetal Origin
The cultivation and characterization of a cell line derived from the foreskin of a fetal, rhesus monkey (rhfs2) are described. This cell line has proven satisfactory for isolation and assay of a variety of viral agents of human and simian origin. Virus titrations performed on foreskin cells yielded titers comparable to, or higher than, those obtained in rhes
-
12. Lipid Metabolism of Monkey Kidney Cells (LLC-MK-2) Infected with Chlamydia trachomatis Strain lymphogranuloma venereum
Lipid metabolism of monkey kidney (LLC-MK-2) cells and cells infected with a Chlamydia trachomatis strain lymphogranuloma venereum (LGV) was studied. The protein-to-lipid ratio of normal MK-2 cells was found to increase linearly over a 60-h period of incubation. The protein-to-lipid ratio of the infected cells was similar to that in normal cells until 36 h a