Lipl32
Mostrando 1-12 de 30 artigos, teses e dissertações.
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1. Diagnosis of canine leptospirosis: evaluation of two PCR assays in comparison with the microagglutination test
RESUMO: O diagnóstico definitivo da leptospirose canina é geralmente realizado demonstrando a seroconversão em amostras do paciente no período agudo e de convalescença por serologia. No entanto, a aplicação de técnicas de PCR pode contribuir para a confirmação de casos suspeitos num período de tempo mais curto. O objetivo deste estudo foi avaliar
Pesq. Vet. Bras.. Publicado em: 02/05/2019
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2. Development and validation of a modified TaqMan based real-time PCR assay targeting the lipl32 gene for detection of pathogenic Leptospira in canine urine samples
Abstract A modified TaqMan real-time polymerase chain reaction targeting a 138 bp fragment within the lipl32 gene was developed to identify exclusively pathogenic Leptospira spp. in dog urine samples. Thirty-five samples from dogs with suspected clinical leptospirosis and 116 samples from apparently healthy dogs were tested for presence of leptospiral DNA us
Braz. J. Microbiol.. Publicado em: 2018-09
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3. COMPARISON OF 16S rRNA-PCR-RFLP, LipL32-PCR AND OmpL1-PCR METHODS IN THE DIAGNOSIS OF LEPTOSPIROSIS
SUMMARY Leptospirosis is still one of the most important health problems in developing countries located in humid tropical and subtropical regions. Human infections are generally caused by exposure to water, soil or food contaminated with the urine of infected wild and domestic animals such as rodents and dogs. The clinical course of leptospirosis is variab
Rev. Inst. Med. trop. S. Paulo. Publicado em: 22/09/2016
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4. The use of halloysite clay and carboxyl-functionalised multi-walled carbon nanotubes for recombinant LipL32 antigen delivery enhanced the IgG response
We studied the feasibility of using halloysite clay nanotubes (HNTs) and carboxyl-functionalised multi-walled carbon nanotubes (COOH-MWCNTs) as antigen carriers to improve immune responses against a recombinant LipL32 protein (rLipL32). Immunisation using the HNTs or COOH-MWCNTs significantly increased the rLipL32-specific IgG antibody titres (p < 0.05) of G
Mem. Inst. Oswaldo Cruz. Publicado em: 23/01/2015
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5. Production of leptospiral LipL32 antigen in Pichia pastoris and its use in an enzyme-linked immunosorbent assay
The production of recombinant LipL32 protein using Escherichia coli has been used extensively for the development of vaccines and diagnostic tests for leptospirosis. However, E. coli has demonstrated limitations, including low yield and lack of post-translational modifications. In this study, rLipL32 was produced in eukaryotic expression system (Pichia pasto
Braz. arch. biol. technol.. Publicado em: 31/01/2014
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6. Expression and purification of the non-tagged LipL32 of pathogenic Leptospira
Leptospirosis is a reemerging infectious disease and the most disseminated zoonosis worldwide. A leptospiral surface protein, LipL32, only occurs in pathogenic Leptospira, and is the most abundant protein on the bacterial surface, being described as an important factor in host immunogenic response and also in bacterial infection. We describe here an alternat
Brazilian Journal of Medical and Biological Research. Publicado em: 2011-04
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7. Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement
The aim of this study was to apply a molecular protocol to detect leptospiral DNA in environmental water samples. The study was carried out in a peri-urban settlement in Petrópolis, state of Rio de Janeiro. A multiplex PCR method employing the primers LipL32 and 16SrRNA was used. Three out of 100 analysed samples were positive in the multiplex PCR, two were
Memórias do Instituto Oswaldo Cruz. Publicado em: 2010-05
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8. Characterization of Leptospira borgpetersenii isolates from field rats (Rattus norvegicus) by 16S rRNA and lipL32 gene sequencing
The main goal of this study was to evaluate the prevalence of leptospirosis among field rodents of Tiruchirappalli district, Tamil Nadu, India. In total 35 field rats were trapped and tested for seroprevalence by the microscopic agglutination test (MAT). Isolation of leptospires was performed from blood and kidney tissues and characterized to serovar level.
Brazilian Journal of Microbiology. Publicado em: 2010-03
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9. Immune strategies using single-component LipL32 and multi-component recombinant LipL32-41-OmpL1 vaccines against leptospira
Genes encoding lipoproteins LipL32, LipL41 and the outer-membrane protein OmpL1 of leptospira were recombined and cloned into a pVAX1 plasmid. BALB/c mice were immunized with LipL32 and recombined LipL32-41-OmpL1 using DNA-DNA, DNA-protein and protein-protein strategies, respectively. Prime immunization was on day 1, boost immunizations were on day 11 and da
Brazilian Journal of Medical and Biological Research. Publicado em: 31/07/2009
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10. Caracterizações biológicas das proteínas LipL32 e HlyX de Leptospira interrogans sorovar Copenhageni. / Biology characterizations of LipL32 and HlyX proteins of Leptospira interrogans sorovar Copenhageni.
Leptospirosis, a spirochaetal zoonotic disease caused by Leptospira, has been recognized as na important emerging infectious disease. LipL32 is a surface lipoprotein which is highly conserved among pathogenic Leptospira species and is also expressed at high levels either during cultivation and natural infection. Regarding HlyX, it has been annotated as a pro
Publicado em: 2009
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11. Study of potential vaccine candidates from Leptospira interrogans serovar Copenhageni. / Estudo de potencias antígenos vacinais de Leptospira interrogans sorovar Copenhageni.
Preventive measures as vaccines are the best strategies to combat leptospirosis. Seven potential vaccine candidates from L. iInterrogans serovar Copenhageni were studied: LipL32, LipL23 and LipL22 (lipoproteins), SphH e Sph4 (hemolysins), AnkB (ankyrin domain) and OmpA76 (OmpA domain). The genes were amplified, cloned and the proteins expressed in E. Coli e
Publicado em: 2009
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12. Molecular identification and serological profile of Leptospira spp. isolated of white-eared-opossum (Didelphis albiventris) in South of Brazil / Identificação molecular e perfil sorológico de Leptospira spp. isolada de gambás-de-orelha-branca (Didelphis albiventris) no sul do Brasil
Leptospirosis is a zoonotic disease that occurs all over the world, particularly in developing countries, caused by bacteria of the genus Leptospira. Several marsupial species are considered susceptible to infection caused by a wide variety of Leptospira serovars, acting as reservoirs. In this work were collected serum and urine samples of 33 White-eared opo
Publicado em: 2009