Lipases Immobilization
Mostrando 1-12 de 21 artigos, teses e dissertações.
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1. Physical-Chemical Properties of the Support Immobead 150 Before and After the Immobilization Process of Lipase
We carried out the physico-chemical characterization of Immobead 150, a hydrophobic support for the immobilization of lipases. Thermomyces lanuginosus lipase (TLL) was immobilized on Immobead 150 by multipoint covalent attachment (ImmTLL) and the morphological, textural, structural, thermal, and physico-chemical properties of the support, before and after en
J. Braz. Chem. Soc.. Publicado em: 2017-08
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2. Easy and Simple SiO2 Immobilization of Lipozyme CaLB-L: Its Use as a Catalyst in Acylation Reactions and Comparison with Other Lipases
In this study, lipase from Candida antarctica B (Lipozyme CaLB-L) was successfully immobilized on SiO2 through adsorption and used to obtain (R)-(+)-esters derived from (R,S)-1-phenylethanol. The new immobilized enzyme was compared with commercially immobilized lipases (Novozyme 435, Lipozyme 435 and Pseudomonas cepacia (PSC-II and PSD-I)). Lipozyme CaLB-L a
J. Braz. Chem. Soc.. Publicado em: 2017-07
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3. Performance of Chitosan/Glycerol Phosphate Hydrogel as a Support for Lipase Immobilization
A biocompatible and biodegradable hybrid chitosan/glycerol phosphate hydrogel (CS-GP) was prepared and evaluated as support for enzyme immobilization. In this work a lipase sn-1,3 specific from Rhizopus oryzae was physically immobilized in CS-GP by adsorption (PA) and in CS-GP modified chemically with glutaraldehyde (CS-GP-GA) by covalent attachment (CA). Th
Mat. Res.. Publicado em: 29/06/2017
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4. Methacrylated Chitosan Based UV Curable Support for Enzyme Immobilization
UV curing is simple, fast and effective procedure for enzyme immobilization with minimized enzymatic activity. UV-curable methacrylated chitosan is here proposed as a support material for immobilization of lipase. The morphology of the polymeric support was characterized by scanning electron microscopy (SEM). Both covalently (CIL) and physically (PIL) immobi
Mat. Res.. Publicado em: 16/02/2017
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5. The Influence of Organic Medium and Supports in the Resolution of (R,S)-Menthol Catalyzed by Lipases
In this study, various commercial and native lipases, free or immobilized, were used in the enantioselective resolution of (R,S)-menthol with vinyl acetate for the preparation of menthyl acetate. Lipase from Candida rugosa (AYL) was found to be the most appropriate. When the reaction was carried out at 35 ºC, using 40 mg of AYL and a molar ratio of 1:2 (alc
J. Braz. Chem. Soc.. Publicado em: 2016-12
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6. Synthesis of structured triacylglycerols enriched in n-3 fatty acids by immobilized microbial lipase
Abstract The search for new biocatalysts has aroused great interest due to the variety of micro-organisms and their role as enzyme producers. Native lipases from Aspergillus niger and Rhizopus javanicus were used to enrich the n-3 long-chain polyunsaturated fatty acids content in the triacylglycerols of soybean oil by acidolysis with free fatty acids from sa
Braz. J. Microbiol.. Publicado em: 2016-12
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7. PERFORMANCE OF LOOFA-IMMOBILIZED Rhizopus oryzae IN THE ENZYMATIC PRODUCTION OF BIODIESEL WITH USE OF OLEIC ACID IN n-HEXANE MEDIUM
AbstractThe performance of loofa-immobilized Rhizopus oryzae (as a whole-cell biocatalyst) in the synthesis of methyl oleate was evaluated using oleic acid as a model substrate. The activities of the cell-bound lipase in terms of the esterification and hydrolysis reactions were found to be higher for the immobilized cells as compared with those of the free c
Braz. J. Chem. Eng.. Publicado em: 2015-06
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8. USO DE SÍLICAS MODIFICADAS PARA IMOBILIZAÇÃO DE LIPASES
Enzyme-support strategies are increasingly replacing conventional chemical methods in both laboratories and industries with attributes including efficiency, higher performance and multifarious use, where silica surfaces show potential due to the chemical bonds based on the presence of hydroxyl groups which can be modified with different additives. Surface-mo
Quím. Nova. Publicado em: 2015-03
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9. Immobilization of Lipases Produced by the Endophytic Fungus Cercospora kikuchii on Chitosan Microparticles
This work studied the immobilization of Cercospora kikuchii lipases on chitosan microparticles by chemical attachment on chitosan acetate microparticles activated by glutaraldehyde (CAM) added before or after the enzyme and physical adsorption on highly deacetylated chitosan hydrochloride microparticles (CHM). Lipases covalently immobilized on pre-activated
Braz. arch. biol. technol.. Publicado em: 2014-08
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10. Epoxidação do β-cariofileno com lipases imobilizadas em gel de ágar
The immobilization of enzymes and microorganisms on solid supports has been developed in recent years. These biocatalysts may be used in organic media allowing their storage and reuse, thus reducing costs of the process. Herein, lipases from various sources were immobilized in agar gel and used as catalysts in the chemo-enzymatic epoxidation of β-caryophyll
Quím. Nova. Publicado em: 2014-07
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11. Imobilização de lipase por encapsulação em sílica aerogel
Lipase from Burkholderia cepacia was immobilized in a silica matrix and dried in high pressure carbon dioxide media (aerogel). The protic ionic liquid (PIL) was used in the immobilization process by encapsulation. The objective of this work was to evaluate the influence of the drying technique using supercritical carbon dioxide in biocatalysts obtained throu
Quím. Nova. Publicado em: 2014-07
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12. Extração, purificação e imobilização de lipases vegetais destinadas à síntese de biodiesel e ésteres
Lipases (glicerol éster hidrolases, EC 3.1.1.3) catalisam reações de hidrólise, esterificação e transesterificação. As lipases podem ser obtidas de fontes animais, microbianas e vegetais, sendo que as de origem microbiana representam a grande maioria das lipases produzidas atualmente. No entanto, o uso dessas enzimas em escala industrial ainda é res
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/03/2012