Ligands Biochemistry
Mostrando 1-10 de 10 artigos, teses e dissertações.
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1. Estudos estruturais e biofísicos da enzima purina nucleosídeo fosforilase hexamérica de Bacillus subtilis / Structural and biophysical studies of hexameric purin nucleoside phosphorylase of Bacillus subtillis
A enzima purina nucleosídeo fosforilase hexamérica de Bacillus subtilis (BsPNP233) c uma nucleosídeo fosforilase do tipo 1 , responsável pela catalise reversível da reação de guebra de urn nucleosídeo em base nitrogenada e ribose-1-fosfato na via de salvação de purinas. Essa enzima possui interesses biomédicos e biotecnológicos devido ao uso na t
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/08/2011
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2. Investigação da atividade biológica de complexos de cobre(II) com ligantes inspirados em biomoléculas / Investigations on the biological activity of copper(II) complexes with ligands inspired in biomolecules
Neste trabalho, alguns novos complexos imínicos de cobre(II) com ligantes inspirados em biomoléculas como oxindóis, contendo grupos indólicos, imidazólicos ou pirrólicos com diferentes características estruturais, foram sintetizados e caracterizados por análise elementar, espectrometria ESI-MS e espectroscopias IV, UVNis e EPR. As possíveis interaç
Publicado em: 2009
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3. Condensation of nucleic acids by intercalating aromatic cations.
Certain intercalating aromatic cations, such as the fluorochrome acridine orange or the antitumor drug Mitoxantrone, induce condensation of nucleic acids in solutions. The appearance of the condensed form during titration of nucleic acids with these intercalating ligands can be quantitatively monitored by light scatter measurements. The resulting highly repr
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4. The role of zinc in the binding of killer cell Ig-like receptors to class I MHC proteins
The binding of killer cell Ig-like Receptors (KIR) to their Class I MHC ligands was shown previously to be characterized by extremely rapid association and dissociation rate constants. During experiments to investigate the biochemistry of receptor–ligand binding in more detail, the kinetic parameters of the interaction were observed to alter dramatica
The National Academy of Sciences.
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5. Single-target molecule detection with nonbleaching multicolor optical immunolabels
We introduce and demonstrate the use of colloidal silver plasmon-resonant particles (PRPs) as optical reporters in typical biological assays. PRPs are ultrabright, nanosized optical scatterers, which scatter light elastically and can be prepared with a scattering peak at any color in the visible spectrum. PRPs are readily observed individually with a microsc
The National Academy of Sciences.
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6. The reaction of platinum(II) complexes with DNA. Kinetics of intrastrand crosslink formation in vitro.
The kinetics of the formation of bifunctional DNA platinum(II) adducts (DNA-crosslinks) have been investigated by endonuclease digestion and subsequent HPLC analysis of the soluble nucleotides and nucleotide platinum(II) adducts. The results indicate two waves of crosslinking [rate constants (0.2-0.3) min-1 and (0.015-0.025) min-1] that correlate with change
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7. Crosslinking of the anticodon of P site bound tRNA to C-1400 of E.coli 16S RNA does not require the participation of the 50S subunit.
Crosslinking of the 5'-anticodon base of ribosomal P site bound AcVal-tRNA to residue C-1400 of 16S RNA or to its equivalent in 18S RNA has been shown to occur on 70S or 80S ribosomes of both prokaryotes and eukaryotes [Ciesiolka, J., Nurse, K., Klein, J. and Ofengand, J. (1985) Biochemistry 24, 3233-3239]. In the present work, we show that the crosslinking
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8. Anandamide and diet: Inclusion of dietary arachidonate and docosahexaenoate leads to increased brain levels of the corresponding N-acylethanolamines in piglets
Endogenous ligands of cannabinoid receptors have been discovered recently and include some N-acylethanolamines (NAEs; e.g., N-arachidonoylethanolamine) and some 2-acylglycerols (e.g., sn-2-arachidonoylglycerol). Previously, we found these compounds to be active biologically when administered per os in large quantities to mice. In the present work, pigl
The National Academy of Sciences.
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9. Mössbauer studies of alkane ω-hydroxylase: Evidence for a diiron cluster in an integral-membrane enzyme
The gene encoding the alkane ω-hydroxylase (AlkB; EC 1.14.15.3) from Pseudomonas oleovorans was expressed in Escherichia coli. The integral-membrane protein was purified as nearly homogeneous protein vesicles by differential ultracentrifugation and HPLC cation exchange chromatography without the detergent solubilization normally required for membrane protei
The National Academy of Sciences of the USA.
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10. Coralyne has a preference for intercalation between TA.T triples in intramolecular DNA triple helices.
Intercalating ligands may improve both the stability and sequence specificity of triple helices. Numerous intercalating drugs have been described, including coralyne, which preferentially binds triple helices, though its sequence specificity has been reported to be low [Lee,J.S., Latimer,L.J.P. and Hampel,K.J. (1993) Biochemistry , 32, 5591-5597]. In order t