Leukocyte Elastase
Mostrando 1-12 de 51 artigos, teses e dissertações.
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1. Evolution of invasive pulmonary aspergillosis produced in mice treated with monoclonal antibodies anti GR-1/Ly-6G and infected with Aspergillus fumigatus strains which presented distincts patterns of production of elastase. / Evolução da aspergilose pulmonar invasiva produzida em camundongos tratados com anticorpos monoclonais anti GR-1/Ly-6G e infectados com amostras de Aspergillus fumigatus que apresentaram distintos padrões de produção de elastase
Invasive Pulmonary Aspergillosis (IPA) is an opportunistic fungal disease, caused mainly by Aspergillus fumigatus, that affects immunocompromised patients. To better understand this mycoses, we originally established in C57BL/6 mice an experimental model of neutrophils depletion by intraperitoneal inoculation of antibodies anti GR-1/Ly-6G, confirmed by total
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 02/04/2012
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2. Impacto da terapia mecânica não cirúrgica em pacientes com periodontite crônica e agressiva generalizadas / Impact of non-surgical periodontal therapy on generalized chronic and aggressive periodontitis
O objetivo deste estudo foi avaliar o impacto do tratamento periodontal não cirúrgico sobre a atividade de elastase e o volume de fluido gengival nos pacientes portadores de periodontite crônica e agressiva generalizadas. Foram avaliados 18 pacientes com periodontite crônica (idade média 48,6 DP 7,5 anos), e 11 com periodontite agressiva (idade média 2
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/02/2010
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3. Pseudomonas aeruginosa elastase does not inactivate alpha 1-proteinase inhibitor in the presence of leukocyte elastase.
Pseudomonas aeruginosa elastase rapidly inactivates alpha 1-proteinase inhibitor by splitting its Pro-357-Met-358 peptide bond. The present study was aimed at testing whether this reaction takes place in the presence of leukocyte elastase. To this end was added alpha 1-proteinase inhibitor to a mixture of the two elastases, and we performed the following ass
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4. Receptor-mediated binding and internalization of leukocyte elastase by alveolar macrophages in vitro.
Radioiodinated leukocyte elastase was found to bind rapidly and specifically to alveolar macrophages in vitro. In contrast to the binding of pancreatic and bacterial proteases, leukocyte elastase binding did not require the presence of alpha 2 macroglobulin. The binding was inhibited by an excess of unlabeled enzyme and was saturable by increasing elastase c
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5. In vitro evidence that human airway lysozyme is cleaved and inactivated by Pseudomonas aeruginosa elastase and not by human leukocyte elastase.
The in vitro effects of Pseudomonas aeruginosa elastase (P. aeruginosa E) and of human leukocyte elastase on human airway lysozyme (HAL) were investigated. P. aeruginosa E inactivated and cleaved HAL, whereas human leukocyte elastase had no effect. Total inactivation of HAL by P. aeruginosa E was observed after 120 min of incubation at 37 degrees C, for an e
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6. Leukocyte Elastase Release during Blood Coagulation: A POTENTIAL MECHANISM FOR ACTIVATION OF THE ALTERNATIVE FIBRINOLYTIC PATHWAY
Immunological detection of elastase, an enzyme present within leukocyte granules, has been used as a marker for polymorphonuclear leukocyte activation. Polymorphonuclear leukocytes contained 4.6 μg/107 cells, whereas erythrocytes, mononuclear cells, and platelets contained <1% of this level. In plasma that was separated from blood cells after 1 h at 22°C,
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7. Isolation, properties, and complete amino acid sequence of human secretory leukocyte protease inhibitor, a potent inhibitor of leukocyte elastase.
A potent inhibitor of human leukocyte elastase (EC 3.4.21.37) and cathepsin G (EC 3.4.21.20) and of human trypsin (EC 3.4.21.4) has been purified from human parotid secretions. The complete amino acid sequence of this protein has been determined. The sequence suggests that the protein has two domains of about 54 amino acids, each of which contains four disul
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8. Leukocyte Elastase Induces Lung Epithelial Apoptosis via a PAR-1–, NF-κB–, and p53-Dependent Pathway
Leukocyte elastase induces apoptosis of lung epithelial cells via alterations in mitochondrial permeability, but the signaling pathways regulating this response remain uncertain. Here we investigated the involvement of proteinase-activated receptor-1 (PAR-1), the transcription factor NF-κB, and the protooncogene p53 in this pathway. Elastase-induced apoptos
American Thoracic Society.
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9. Human leukocyte elastase, cathepsin G, and lactoferrin: family of neutrophil granule glycoproteins that bind to an alveolar macrophage receptor.
Interactions between polymorphonuclear neutrophils and mononuclear phagocytes are potentially of great importance in a variety of inflammatory processes. As part of a continuing effort to elucidate the physiologic importance of human alveolar macrophage receptor-mediated binding of neutrophil (leukocyte) elastase, I have studied the binding of leukocyte elas
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10. The role of secretory leukocyte proteinase inhibitor and elafin (elastase-specific inhibitor/skin-derived antileukoprotease) as alarm antiproteinases in inflammatory lung disease
Secretory leukocyte proteinase inhibitor and elafin are two low-molecular-mass elastase inhibitors that are mainly synthesized locally at mucosal sites. It is thought that their physicochemical properties allow them to efficiently inhibit target enzymes, such as neutrophil elastase, released into the interstitium. Historically, in the lung, these inhibitors
BioMed Central.
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11. L-DNase II, a Molecule That Links Proteases and Endonucleases in Apoptosis, Derives from the Ubiquitous Serpin Leukocyte Elastase Inhibitor
American Society for Microbiology.
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12. Cleavage and inactivation of Factor IX by granulocyte elastase.
Radioiodinated Factor IX was cleaved by a crude sonicate from leukocytes. In the absence of calcium, fragments of less than 15,000 mol wt were seen from reduced samples on gel electrophoresis. After digestion in 2 mM calcium, however, electrophoresis of reduced samples showed, in addition to low molecular weight fragments, protein bands corresponding in size