Lens Epithelial Cells
Mostrando 13-24 de 66 artigos, teses e dissertações.
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13. Inhibition of chicken embryo lens differentiation and lens junction formation in culture by pp60v-src.
A culture system was developed which permitted the differentiation of chicken lens epithelial cells to lentoid bodies which contained several cell layers, accumulated high levels of delta-crystallin, and produced extensive gap junctions. This differentiation process was prevented when the cells were infected with a temperature-sensitive src mutant of Rous sa
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14. 7-Ketocholesterol modulates intercellular communication through gap-junction in bovine lens epithelial cells
BioMed Central.
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15. Expression of the intermediate-filament-associated protein synemin in chicken lens cells.
Synemin, a 230-kilodalton polypeptide component of avian muscle and erythrocyte intermediate filaments, is also found in association with the vimentin filaments of lens tissue. In chicken lens cells, synemin is bound to the core vimentin polymer with the same 180-nm periodicity that it exhibits in erythrocytes. Its solubility properties are characteristic of
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16. Types of collagen synthesised by the lens epithelial cells of human cataracts.
AIMS/BACKGROUND--Residual lens epithelial cells (LECs) undergo fibrous proliferation after cataract surgery, resulting in capsular fibrosis. The purpose of this study was to determine the types of collagen produced in cultured LECs derived from human cataract LECs. METHODS--A circular section of the anterior capsule, about 5 mm in diameter, with LECs attache
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17. A forkhead gene, FoxE3, is essential for lens epithelial proliferation and closure of the lens vesicle
In the mouse mutant dysgenetic lens (dyl) the lens vesicle fails to separate from the ectoderm, causing a fusion between the lens and the cornea. Lack of a proliferating anterior lens epithelium leads to absence of secondary lens fibers and a dysplastic, cataractic lens. We report the cloning of a gene, FoxE3, encoding a forkhead/winged helix transcription f
Cold Spring Harbor Laboratory Press.
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18. Taxon-specific recruitment of enzymes as major soluble proteins in the corneal epithelium of three mammals, chicken, and squid.
Studies of others have shown that class 3 aldehyde dehydrogenase is a major component of the epithelial cells of the mammalian cornea. Here we demonstrate by peptide sequencing that other major proteins of the corneal epithelium are also identical or related to enzymes in the human, mouse, kangaroo, chicken, and squid. Aldehyde dehydrogenase class 3 was foun
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19. Lentropin: a factor in vitreous humor which promotes lens fiber cell differentiation.
An activity has been identified in chicken vitreous humor which stimulates embryonic chicken lens epithelial cells to elongate and specialize for lens crystallin synthesis. The activity is heat-labile and is destroyed by treatment with trypsin or agents that reduce disulfides. Gel filtration and ultrafiltration analyses indicate that it has an apparent molec
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20. Pax6 is essential for lens fiber cell differentiation
The developing ocular lens provides an excellent model system with which to study the intrinsic and extrinsic cues governing cell differentiation. Although the transcription factors Pax6 and Sox2 have been shown to be essential for lens induction, their later roles during lens fiber differentiation remain largely unknown. Using Cre/loxP mutagenesis, we
Company of Biologists.
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21. Lentropin, a protein that controls lens fiber formation, is related functionally and immunologically to the insulin-like growth factors.
Lentropin, a factor present in the vitreous humor of the eye, stimulates lens fiber differentiation from chicken embryo lens epithelial cells in vitro. Lentropin has been partially purified but has not been isolated in sufficient quantity or purity for direct comparison with other growth and differentiation factors. Previous studies have shown that insulin a
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22. DNA sequences responsible for tissue-specific expression of a chicken alpha-crystallin gene in mouse lens cells.
We have studied the DNA sequences required for high-level expression of a cloned chicken alpha-crystallin gene by introducing a hybrid alpha/delta-crystallin gene into nuclei of mouse lens epithelial cells in primary culture. The level of transient expression of the hybrid gene consisting of the 5' upstream promoter region of the alpha-crystallin gene fused
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23. Dual Roles for Pax-6: a Transcriptional Repressor of Lens Fiber Cell-Specific β-Crystallin Genes
It has been demonstrated previously that Pax-6, a paired domain (PD)/homeodomain (HD) transcription factor critical for eye development, contributes to the activation of the αB-, αA-, δ1-, and ζ-crystallin genes in the lens. Here we have examined the possibility that the inverse relationship between the expression of Pax-6 and β-crystallin genes within
American Society for Microbiology.
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24. The retinoblastoma protein-binding region of simian virus 40 large T antigen alters cell cycle regulation in lenses of transgenic mice.
Regulation of the cell cycle is a critical aspect of cellular proliferation, differentiation, and transformation. In many cell types, the differentiation process is accompanied by a loss of proliferative capability, so that terminally differentiated cells become postmitotic and no longer progress through the cell cycle. In the experiments described here, the