Laser Focusing
Mostrando 13-19 de 19 artigos, teses e dissertações.
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13. Picosecond resonance Raman spectroscopic evidence for excited-state spin conversion in carbonmonoxy-hemoglobin photolysis
The structure of the carbonmonoxy-hemoglobin (COHb) photoproduct has been studied on the picosecond time scale with resonance Raman spectroscopy, by tightly focusing the 30-ps pulses of a synchronously pumped mode-locked cavitydumped dye laser on a jet stream of COHb solution. The spectrum of the photoproduct is similar to that of deoxy Hb, but the frequenci
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14. The emergence of mass spectrometry in biochemical research.
The initial steps toward routinely applying mass spectrometry in the biochemical laboratory have been achieved. In the past, mass spectrometry was confined to the realm of small, relatively stable molecules; large or thermally labile molecules did not survive the desorption and ionization processes intact. Electrospray ionization (ESI) and matrix-assisted la
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15. Extracellular Arginine Aminopeptidase from Streptococcus gordonii FSS2
Streptococcus gordonii is a primary etiological agent in the development of subacute bacterial endocarditis (SBE), producing thrombus formation and tissue damage on the surfaces of heart valves. This is ironic, considering its normal role as a benign inhabitant of the oral microflora. However, strain FSS2 of S. gordonii has been found to produce several extr
American Society for Microbiology.
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16. Why are blue visual pigments blue? A resonance Raman microprobe study.
A resonance Raman microscope has been developed to study the structure of the retinal prosthetic group in the visual pigments of individual photoreceptor cells. Raman vibrational spectra are obtained by focusing the probe laser on intact photoreceptors frozen on a 77 K cold stage. To elucidate the mechanism of wavelength regulation in blue visual pigments, w
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17. The secretory granule matrix-electrolyte interface: a homologue of the p-n rectifying junction.
When placed at the tip of a glass micropipette electrode the polymeric matrix of the secretory granule behaves like a diode. The measured current was 100-fold greater at negative potentials compared to positive potentials, and up to sixfold greater than that measured with the pipette alone. By manipulating the geometry of the electric field we show that thes
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18. Rapid Deposition of Extensin during the Elicitation of Grapevine Callus Cultures Is Specifically Catalyzed by a 40-Kilodalton Peroxidase1
Elicitation or peroxide stimulation of grape (Vitis vinifera L. cv Touriga) vine callus cultures results in the rapid and selective in situ insolubilization of an abundant and ionically bound cell wall protein-denominated GvP1. Surface-enhanced laser desorption/ionization/time of flight-mass spectrometry analysis, the amino acid composition, and the N-termin
American Society of Plant Physiologists.
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19. Barley Coleoptile Peroxidases. Purification, Molecular Cloning, and Induction by Pathogens1
A cDNA clone encoding the Prx7 peroxidase from barley (Hordeum vulgare L.) predicted a 341-amino acid protein with a molecular weight of 36,515. N- and C-terminal putative signal peptides were present, suggesting a vacuolar location of the peroxidase. Immunoblotting and reverse-transcriptase polymerase chain reaction showed that the Prx7 protein and mRNA acc
American Society of Plant Physiologists.