Lactose Permease
Mostrando 1-12 de 123 artigos, teses e dissertações.
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1. Relationship among Shigella spp. and enteroinvasive Escherichia coli (EIEC) and their differentiation
Shigellosis produces inflammatory reactions and ulceration on the intestinal epithelium followed by bloody or mucoid diarrhea. It is caused by enteroinvasive E. coli (EIEC) as well as any species of the genus Shigella, namely, S. dysenteriae, S. flexneri, S. boydii, and S. sonnei. This current species designation of Shigella does not specify genetic similari
Braz. J. Microbiol.. Publicado em: 2014-12
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2. Influence of carbon metabolism and oxygen level on lactose permease localization in Kluyveromyces lactis / Influência do metabolismo de carbono e do nível de oxigênio sobre a localização da permease de lactose em Kluyveromyces lactis
In this work we analyzed the influence of the carbon source, oxygen level and snf1 mutation on the subcelular localization of Lac12GFP permease in the yeast Kluyveromyces lactis. Initially we showed that K. lactis has higher affinity for lactose than for glucose. Our data suggest that the KlSnf1p dependent subcelular localization of Lac12GFP permease was not
Publicado em: 2007
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3. Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey
Saccharomyces cerevisiae strain W303 was transformed with two yeast integrative plasmids containing Kluyveromyces lactis LAC4 and LAC12 genes that codify beta-galactosidase and lactose permease respectively. The BLR030 recombinant strain was selected due to its growth and beta-galactosidase production capacity. Different culture media based on deproteinized
Brazilian Journal of Chemical Engineering. Publicado em: 2006-12
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4. Regulation of lactose permease activity by the phosphoenolpyruvate:sugar phosphotransferase system: evidence for direct binding of the glucose-specific enzyme III to the lactose permease.
Interaction between the glucose-specific enzyme III (enzyme IIIglc) of the phosphoenolpyruvate:sugar phosphotransferase system and the lactose permease was studied with membrane fragments from an Escherichia coli strain that overproduces the lactose permease. Substrates of the permease markedly and specifically stimulated binding of enzyme IIIglc to the memb
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5. Transport by the lactose permease of Escherichia coli as the basis of lactose killing.
Lactose killing is a peculiar phenomenon in which 80 to 98% of the Escherichia coli cells taken from a lactose-limited chemostat die when plated on standard lactose minimal media. This unique form of suicide is caused by the action of the lactose permease. Since uptake of either lactose or galactose by the lactose permease caused death, the action of rapid t
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6. Topology of allosteric regulation of lactose permease
Sugar transport by some permeases in Escherichia coli is allosterically regulated by the phosphorylation state of the intracellular regulatory protein, enzyme IIAglc of the phosphoenolpyruvate:sugar phosphotransferase system. A sensitive radiochemical assay for the interaction of enzyme IIAglc with membrane-associated lactose permease was used to characteriz
The National Academy of Sciences of the USA.
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7. Mutations in the lacY gene of Escherichia coli define functional organization of lactose permease.
Mutations in the lacY gene of Escherichia coli have been used to analyze the functional organization of lactose permease. Deletions suggest that the NH2 terminus of lactose permease is not essential and can be replaced by residues of the cytoplasmic enzyme beta-galactosidase. Negative dominant mutations in the lacY gene can be explained by the assumption tha
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8. The size of the lactose permease derived from rotational diffusion measurements.
The lactose permease of Escherichia coli was labeled with eosinyl-maleimide, reconstituted into vesicles of dimyristoylphosphatidylcholine and subjected to time-dependent phosphorescence anisotropy measurements in order to determine the rotational diffusion coefficient. By comparison with bacteriorhodopsin, the diffusion coefficient is evaluated in terms of
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9. Involvement of the central loop of the lactose permease of Escherichia coli in its allosteric regulation by the glucose-specific enzyme IIA of the phosphoenolpyruvate-dependent phosphotransferase system.
Allosteric regulation of several sugar transport systems such as those specific for lactose, maltose and melibiose in Escherichia coli (inducer exclusion) is mediated by the glucose-specific enzyme IIA (IIAGlc) of the phosphoenolpyruvate:sugar phosphotransferase system (PTS). Deletion mutations in the cytoplasmic N and C termini of the lactose permease prote
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10. Cooperative Binding of the Sugar Substrates and Allosteric Regulatory Protein (Enzyme IIIGlc of the Phosphotransferase System) to the Lactose and Melibiose Permeases in Escherichia coli and Salmonella typhimurium
An Escherichia coli strain which overproduces the lactose permease was used to investigate the mechanism of allosteric regulation of this permease and those specific for melibiose, glycerol, and maltose by the phosphoenolpyruvate-sugar phosphotransferase system (PTS). Thio-β-digalactoside, a high affinity substrate of the lactose permease, released the glyc
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11. Properties of permease dimer, a fusion protein containing two lactose permease molecules from Escherichia coli.
An engineered fusion protein containing two tandem lactose permease molecules (permease dimer) exhibits high transport activity and is used to test the phenomenon of negative dominance. Introduction of the mutation Glu-325-->Cys into either the first or the second half of the dimer results in a 50% decrease in activity, whereas introduction of the mutation i
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12. Lactose metabolism in Lactobacillus bulgaricus: analysis of the primary structure and expression of the genes involved.
The genes coding for the lactose permease and beta-galactosidase, two proteins involved in the metabolism of lactose by Lactobacillus bulgaricus, have been cloned, expressed, and found functional in Escherichia coli. The nucleotide sequences of these genes and their flanking regions have been determined, showing the presence of two contiguous open reading fr