L Amino Acid Oxidase
Mostrando 25-36 de 116 artigos, teses e dissertações.
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25. Caracterização bioquímica e funcional de uma nova L-aminoácido oxidase isolada da peçonha da serpente Bothrops pirajai
In this work we describe the isolation of a new L-amino acid oxidase (LAAO) referred to as BpirLAAO-I from Bothrops pirajai snake venom, which was highly purified using a combination of molecular exclusion, affinity and hydrophobic chromatography steps. BpirLAAO-I is a homodimeric acidic glycoprotein (approximate Mr and pI of 130,000 and 4.9, respectively),
Publicado em: 2007
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26. Isolamento e caracterização de um novo conjunto de serinoproteases com atividade trombina-like e de L-aminoacido oxidase do veneno de Crotalus durissus cascavella / Isolation and characterization of a new serine proteases group with thrombinin-like and L-amino acid oxidase activity from Crotalus durissus cascavella
The use of the isolated toxins from poisons as molecular tools in the understanding of diverse physiological and pathological events has been proved by some works in literature. The serpent Crotalus durissus cascavella is found in the areas of Caatinga Northeast of Brazil, since Maranhão until North of the state of Minas Gerais, and in spite of it hasn?t be
Publicado em: 2006
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27. Caracterização funcional e estrutural de uma L-Aminoácido oxidace do veneno de Bothrops jararacussue avaliação da sua ação antitumoral, antiparasitária e bactericida / Functional and structural characterization of an L-aminoacid oxidace from Bothrops jararacussuvenom and the evaluation of its antitumoral, antiparasitic and bactericidal action
In this research we described the isolation and biochemical, structural and functional characterization of an L-amino acid oxidase from Bothrops jararacussu venom, named BjussuLAAO. The purification was carried out through three chromatography steps, using a molecular filtration on Sephadex G-75, followed by an affinity chromatography on Benzamidine-Sepharos
Publicado em: 2006
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28. Effects of Bothrops insularis venom and its isolated fractions on renal and vascular systems / Avaliação dos efeitos renais e vasculares do veneno da Bothrops insularis e de frações isoladas
Foram investigados os efeitos do veneno da serpente Bothrops insularis e de suas frações, lectina, L-aminoácido oxidase, trombina símile e fosfolipase A2, no rim isolado e sistema vascular de rato. As frações foram purificadas a partir de uma combinação de procedimentos cromatográficos, usando colunas de HPLC de exclusão molecular, troca iônica, f
Publicado em: 2006
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29. Demonstration of Imino Acids as Products of the Reactions Catalyzed by D- and L-Amino Acid Oxidases
It had long been thought, but never demonstrated, that imino acids are formed in the reactions catalyzed by D- and L-amino acid oxidases (EC 1.4.3.3 and 1.4.3.2). The formation of imino acids is now shown directly by allowing the amino acid oxidase reaction to proceed in the presence of NaBH4, when the imino acid is reduced to the corresponding racemic amino
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30. d-Amino Acid Oxidase Induction in the Kidneys of Germ-free Mice
High levels of d-amino acid oxidase activity were found in the kidneys of conventionally reared mice, whereas the enzyme was absent from the kidneys of most germ-free mice. Injection of d-alanine or monocontamination with Bacillus cereus stimulated d-amino acid oxidase activity in the kidneys of germ-free mice.
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31. Amino acid oxidase of leukocytes in relation to H2O2-mediated bacterial killing
D-Amino acid oxidase and L-amino acid oxidase have been measured in sucrose homogenates of polymorphonuclear leukocytes (PMN) obtained from guinea pigs and humans. Subcellular distribution patterns and studies on latency indicate that these oxidases are soluble enzymes. Their hydrogen peroxide-generating capacity was verified. Chronic granulomatous disease P
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32. Amino Acid Oxidase in Leukocytes: Evidence Against a Major Role in Phagocytosis
The addition of either d- or l-amino acids fails to increase hexose monophosphate shunt activity of resting or phagocytizing neutrophils. This is presumptive evidence against a major role for amino acid oxidase in the bactericidal activity of the cell.
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33. Isolation, crystallization and preliminary X-ray diffraction analysis of l-amino-acid oxidase from Vipera ammodytes ammodytes venom
A novel l-amino-acid oxidase was isolated from V. ammodytes ammodytes venom and crystallized. The solution conditions under which the protein sample was monodisperse were optimized using dynamic light scattering prior to crystallization. Preliminary diffraction data were collected to 2.6 Å resolution.
International Union of Crystallography.
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34. Role of D-tryptophan oxidase in D-tryptophan utilization by Escherichia coli.
Mutants of Escherichia coli K-12 that require L-tryptophan (trp) are normally unable to utilize D-tryptophan to fulfill their requirement. However, secondary mutations (dadR) that confer this ability can be isolated. In such strains two distinct enzymes are found to be produced at high levels: D-amino acid oxidase (EC 1.4.3.3) and D-tryptophan oxidase. A con
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35. Reactions of cysteamine and other amine metabolites with glyoxylate and oxygen catalyzed by mammalian D-amino acid oxidase.
Pig kidney D-amino acid oxidase [D-amino-acid:oxygen oxidoreductase (deaminating), EC 1.4.3.3] catalyzes a rapid uptake of oxygen when high concentrations (50-100 mM) of glyoxylate and the following amines are present under usual assay conditions (pH 8.3): cysteamine, 2-aminoethanol, putrescine, D,L-1-amino-2-propanol, D,L-2-amino-1-propanol, 3-amino-1-propa
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36. Alkylamine-Dependent Amino-Acid Oxidation by Lysine Monooxygenase—Fragmented Substrate of Oxygenase
Lysine monooxygenase catalyzes the oxygenative decarboxylation of L-lysine and produces a corresponding acid amide. L-Alanine was inactive as substrate. However, when propylamine was present, oxidation, but not oxygenation, of alanine was demonstrated with the oxygenase. Alanine was converted to pyruvate, with the liberation of ammonia and hydrogen peroxide,