Kinetoplastid
Mostrando 1-12 de 101 artigos, teses e dissertações.
-
1. Exploring the environmental diversity of kinetoplastid flagellates in the high-throughput DNA sequencing era
The class Kinetoplastea encompasses both free-living and parasitic species from a wide range of hosts. Several representatives of this group are responsible for severe human diseases and for economic losses in agriculture and livestock. While this group encompasses over 30 genera, most of the available information has been derived from the vertebrate pathoge
Mem. Inst. Oswaldo Cruz. Publicado em: 24/11/2015
-
2. Whole-cell antigenicity data support sequence-based kinetoplastid taxonomy
Early immunological data, obtained by immunodiffusion and immunoelectrophoresis, on the whole-cell antigenicity of kinetoplastid protozoa were retrieved and used to construct a dendrogram of antigenic distances. Remarkably, they supported the same taxonomic conclusions as analyses based on DNA and protein sequence data.
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-04
-
3. Kinetoplastid membrane protein-11 exacerbates infection with Leishmania amazonensis in murine macrophages
In Leishmania amazonensis, kinetoplastid membrane protein-11 (KMP-11) expression increases during metacyclogenesis and is higher in amastigotes than in promastigotes, suggesting a role for this protein in the infection of the mammalian host. We show that the addition of KMP-11 exacerbates L. amazonensis infection in peritoneal macrophages from BALB/c mice by
Memórias do Instituto Oswaldo Cruz. Publicado em: 2012-03
-
4. Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesis
Kinetoplastid membrane protein-11 (KMP-11), a protein present in all kinetoplastid protozoa, is considered a potential candidate for a leishmaniasis vaccine. A suitable leishmaniasis vaccine candidate molecule must be expressed in amastigotes, the infective stage for mammals. However, the expression of KMP-11 in Leishmania amastigotes has been a subject of c
Memórias do Instituto Oswaldo Cruz. Publicado em: 2010-05
-
5. Caracterização bioquímica de uma Leucil-aminopeptidase de Trypanosoma cruzi (LAPTc)
Pathogens depend on peptidase activities to accomplish many physiological processes, as well as to interact with their hosts, highlighting parasitic peptidases as virulence factors and, thus, potential drug targets. The kinetoplastid Trypanosoma cruzi genome sequencing, the aetiological agent of Chagas disease, has revealed 28 genes encoding putative aminope
Publicado em: 2010
-
6. Genus-specific kinetoplast-DNA PCR and parasite culture for the diagnosis of localised cutaneous leishmaniasis: applications for clinical trials under field conditions in Brazil
The positivities of two methods for the diagnosis of localised cutaneous leishmaniasis (CL) were estimated in 280 patients enrolled in a clinical trial. The trial was conducted in an endemic area of Leishmania (Viannia) braziliensis and trial participants were patients with skin ulcers and positive leishmanin skin tests. Patients underwent aspirative skin pu
Memórias do Instituto Oswaldo Cruz. Publicado em: 2009-11
-
7. Estudos moleculares das enzimas Fosfoseril-tRNA sintease de Trypanosoma brucei e Leishmania major e Seril-tRNA sintease de Trypanosoma brucei / Molecular studies the enzymes Fosfoseril-tRNA Kinase of the Trypanosoma brucei and Leishmania major and Seril-tRNA Sintetase of the Trypanosoma brucei
The translation process study is central role in the cellular metabolism and attracts the interest of several groups, in particular, the study of the 21º amino acid, the selenocystein. The selenocystein incorporation pathway was described in Escherichia coli and recently in eukaryotes. The first step of this pathway is initiated by Seryl-tRNA Synthetase tha
Publicado em: 2009
-
8. Estudos moleculares das enzimas envolvidas na biossíntese de selenocisteína em Trypanosoma brucei e Leishmania major / Molecular studies of the enzymes involved in selenocysteine synthesis in Trypanosoma brucei and Leishmania major
One of the main biological forms of the selenium incorporation is the amino acid form named selenocysteine (Sec, U), which is incorporated co-translationally at the emerging new polypeptide in the specific positions at the UGA codon, that is usually recognized as stop codon. The incorporation of the selenocysteine in E.coli is already solved with the involve
Publicado em: 2008
-
9. Phylogeny of the kinetoplastida: taxonomic problems and insights into the evolution of parasitism
To further investigate phylogeny of kinetoplastid protozoa, the sequences of small subunit (18S) ribosomal RNA of nine bodonid isolates and ten isolates of insect trypanosomatids have been determined. The root of the kinetoplastid tree was attached to the branch of Bodo designis and/or Cruzella marina. The suborder Trypanosomatina appeared as a monophyletic
Memórias do Instituto Oswaldo Cruz. Publicado em: 2001-04
-
10. The monogenetic kinetoplastid protozoan, Crithidia fasciculata, contains a transcriptionally active, multicopy mini-exon sequence.
A repeated sequence from the Crithidia fasciculata nuclear genome has been isolated which is homologous to the mini-exon genes of other kinetoplastid protozoa. Sequence analysis of the 417 bp monomeric unit confirmed the presence of a 35 nt sequence within the repeat that is 77% homologous with the Trypanosoma brucei 35-mer mini-exon or spliced leader sequen
-
11. Telomerase in kinetoplastid parasitic protozoa
We have identified telomerase activity in extracts of three evolutionarily diverse kinetoplastid species: Trypanosoma brucei, Leishmania major, and Leishmania tarentolae. Telomerase activity was initially detected in extracts from insect form cells of all three kinetoplastid species by using a modification of the one-tube telomere repeat amplification protoc
The National Academy of Sciences.
-
12. Exportin 1 Mediates Nuclear Export of the Kinetoplastid Spliced Leader RNA
The kinetoplastid protozoan spliced leader (SL) RNA is the common substrate pre-mRNA utilized in all trans-splicing reactions. Here we show by fluorescence in situ hybridization that the SL RNA is present in the cytoplasm of Leishmania tarentolae and Trypanosoma brucei. Treatment with the karyopherin-specific inhibitor leptomycin B was toxic to T. brucei and
American Society for Microbiology.