Is6110
Mostrando 1-12 de 327 artigos, teses e dissertações.
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1. Improvement of Mycobacterium tuberculosis detection in sputum using DNA extracted by sonication
Abstract Tuberculosis (TB) is one of the infectious diseases with high mortality in the world. DNA amplification techniques have been used to overcome barriers to the diagnosis of this disease. However, the success of these methodologies is highly dependent on the DNA obtained from the sample. This study was carried out to verify whether the DNA extracted by
Braz J Infect Dis. Publicado em: 2020-10
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2. Mathematical models to predict growth, fillet traits, and composition of wild traíra, Hoplias malabaricus
ABSTRACT This study aimed to determine the length-weight relationship and mathematical models to predict dressed and fillet weight and yield and fillet composition of wild traíra, Hoplias malabaricus (Bloch, 1794). A total of 80 marketable-sized fish from 292.28 to 2879.57 g and 32.06 to 61.19 cm were used. The length:weight ratio was estimated using the eq
R. Bras. Zootec.. Publicado em: 25/11/2019
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3. Usefulness of 3’- 5’ IS6110-RFLP genotyping and spoligotyping of Mycobacterium tuberculosis isolated in a tertiary hospital: a retrospective study detecting unsuspected epidemiological events
ABSTRACT A drug resistance survey involving Mycobacterium tuberculosis isolated from patients of a tertiary Hospital in the Rio de Janeiro city (RJ), Brazil, between the years 1996 and 1998 revealed a high frequency of isoniazid (HR) resistance. These isolates were revisited and genotyped. Patients came from different RJ neighborhoods and municipalities, and
Rev. Inst. Med. trop. S. Paulo. Publicado em: 12/09/2019
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4. Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil
Abstract DNA genotyping of Mycobacterium tuberculosis has been widely applied in the understanding of disease transmission in many countries. The purpose of this study was to genotype the strains of M. tuberculosis isolated in patients with new tuberculosis (TB) cases in Minas Gerais, as well as to compare the similarity, discriminatory power, and agreement
Braz. J. Microbiol.. Publicado em: 2018-09
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5. Performance of the IS6110-TaqMan® assay in the diagnosis of extrapulmonary tuberculosis from different biological samples
Abstract INTRODUCTION: This study evaluated the performance of the IS6110-TaqMan® assay in different types of biological samples and tissues for laboratory diagnosis of extrapulmonary tuberculosis. METHODS: 143 biological samples and tissues from patients with suspected extrapulmonary tuberculosis from the health services of Recife/Pernambuco/Brazil were
Rev. Soc. Bras. Med. Trop.. Publicado em: 2018-06
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6. Tuberculose em felinos domésticos (Felis catus) no sul do Rio Grande do Sul
RESUMO: Descrevem-se os aspectos clínico patológicos de quatro casos de tuberculose em felinos domésticos no Sul do Rio Grande do sul. Foi realizado um estudo retrospectivo dos casos diagnosticados como tuberculose em gatos necropsiados no Laboratório Regional de Diagnóstico da Universidade Federal de Pelotas (LRD/UFPel) no período de janeiro de 2000 a
Pesq. Vet. Bras.. Publicado em: 2017-07
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7. Utility of multiplex real-time PCR in the diagnosis of extrapulmonary tuberculosis
Abstract Objective The diagnosis of extrapulmonary tuberculosis is still a challenge because of its pauci-bacillary nature. The aim of the study was to evaluate the role of a multiplex PCR assay in the diagnosis of extrapulmonary tuberculosis and to compare the efficiency of two targets, IS6110 and MPB64 to detect Mycobacterium tuberculosis. Methods 150 ex
Braz J Infect Dis. Publicado em: 2016-06
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8. Molecular characterization of Mycobacterium tuberculosis isolates from Tehran, Iran by restriction fragment length polymorphism analysis and spoligotyping
Abstract: INTRODUCTION Characterization of Mycobacterium tuberculosis (MTB) isolates by DNA fingerprinting has contributed to tuberculosis (TB) control. The aim of this study was to determine the genetic diversity of MTB isolates from Tehran province in Iran. METHODS MTB isolates from 60 Iranian and 10 Afghan TB patients were fingerprinted by standard IS
Rev. Soc. Bras. Med. Trop.. Publicado em: 2016-04
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9. Identificação e genotipagem de Mycobacterium bovis em bovinos positivos no teste intradérmico para tuberculose em Mato Grosso do Sul
Neste estudo, realizou-se genotipagem de isolados de Mycobacterium bovis, provenientes de amostras de tecidos de bovinos positivos no teste cervical comparativo (TCC) para tuberculose em Mato Grosso do Sul, por meio da técnica de spoligotyping. Tecidos de 13 bovinos positivos, oriundos de diferentes municípios do estado, foram cultivados em meio de Stonebr
Pesq. Vet. Bras.. Publicado em: 2015-02
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10. Use of conventional PCR and smear microscopy to diagnose pulmonary tuberculosis in the Amazonian rainforest area
The diagnostic usefulness of Ziehl-Neelsen (ZN)-stained sputum smears combined with conventional polymerase chain reaction (ZN/PCR) to amplify IS6110 region DNA extracted from ZN slides was evaluated. The objective was to verify if this association could improve tuberculosis (TB) diagnosis in patients at remote sites. The study was carried out in 89 patients
Braz J Med Biol Res. Publicado em: 16/09/2014
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11. Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from othe
Braz. J. Microbiol.. Publicado em: 2014-09
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12. Correlations between major risk factors and closely related Mycobacterium tuberculosis isolates grouped by three current enotyping procedures: a population-based study in northeast Mexico
The characteristics of tuberculosis (TB) patients related to a chain of recent TB transmissions were investigated. Mycobacterium tuberculosis (MTB) isolates (120) were genotyped using the restriction fragment length polymorphism-IS6110 (R), spacer oligotyping (S) and mycobacterial interspersed repetitive units-variable number of tandem repeats (M) methods. T
Mem. Inst. Oswaldo Cruz. Publicado em: 2014-09