Internalin A
Mostrando 1-12 de 35 artigos, teses e dissertações.
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1. Produção e caracterização de anticorpos monoclonais contra InlA de Listeria monocytogenes / Production and characterization of monoclonal antibodies against InlA from Listeria monocytogenes
O patógeno alimentar Listeria monocytogenes é o agente causador da listeriose, uma doença severa que cursa com altas taxas de morbidade e de mortalidade. Os métodos convencionais empregados para detecção desta bactéria são laboriosos e onerosos, requerendo vários dias para sua identificação final. Imunoensaios usados para detecção rápida desta
Publicado em: 2008
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2. Perfil genotípico de cepas de Listeria monocytogenes isoladas de alimentos: análise crítica das técnicas de PCR e PFGE e importância para a Saúde Pública. / Genotype profile of cepas of would listeria monocytogenes isolated of foods: critical analysis of the techniques of PCR and PFGE and importance for the public health.
Due to the seriousness of clinical manifestations and high rates of mortality of the listeriosis disease in populations at risk, control and prevention of this disease, caused by L. monocytogenes, represent an important challenge to sanitation authorities. Difficulties of recovering organisms involved in outbreaks; sub notification; and, the delay in accompl
Publicado em: 2006
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3. The inlA Gene of Listeria monocytogenes LO28 Harbors a Nonsense Mutation Resulting in Release of Internalin
Internalin is a surface protein that mediates entry of Listeria monocytogenes EGD into epithelial cells expressing the cell adhesion molecule human E-cadherin or its chicken homolog, L-CAM, which act as receptors for internalin. After observing that entry of L. monocytogenes LO28 into S180 fibroblasts, in contrast to that of EGD, did not increase after trans
American Society for Microbiology.
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4. Internalin of Listeria monocytogenes with an intact leucine-rich repeat region is sufficient to promote internalization.
Listeria monocytogenes can use two different surface proteins, internalin (InlA) and InlB, to invade mammalian cells. The exact role of these invasiveness factors in vivo remains to be determined. In cultured cells, InlA is necessary to promote Listeria entry into human epithelial cells, such as Caco-2 cells, whereas InlB is necessary to promote Listeria int
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5. Truncated Internalin A and Asymptomatic Listeria monocytogenes Carriage: In Vivo Investigation by Allelic Exchange
Allelic exchange of the region coding for the C terminus of InlA between one epidemic (with an 80-kDa InlA) and one asymptomatic (with a 47-kDa InlA) carriage Listeria monocytogenes strain confirmed the need for this region for internalin entry in vitro. Interestingly, restoration of internalin A functionality did not result in full virulence in chicken embr
American Society for Microbiology.
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6. Antibodies to the leucine-rich repeat region of internalin block entry of Listeria monocytogenes into cells expressing E-cadherin.
Internalin, a surface protein essential for entry of Listeria monocytogenes EGD into epithelial cells, was used as an antigen to raise nine monoclonal antibodies. These monoclonal antibodies recognized seven distinct epitopes which were located in three different regions of the protein. Three of them inhibited internalin-mediated entry and recognized the ami
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7. Use of Listeriolysin O and Internalin A in a Seroepidemiological Study of Listeriosis in Swiss Dairy Cows
Recombinant listeriolysin O and internalin A were used as antigens in an enzyme-linked immunosorbent assay (ELISA) for the specific detection of anti-Listeria monocytogenes antibodies in cattle. The results showed sensitivities and specificities of 82 and 92%, respectively, for the listeriolysin O ELISA, and 100 and 90%, respectively, for the internalin A EL
American Society for Microbiology.
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8. Identification of four new members of the internalin multigene family of Listeria monocytogenes EGD.
Listeria monocytogenes is a bacterial pathogen that is able to invade nonphagocytic cells. Two surface proteins, internalin, the inlA gene product, and InlB, play important roles in the entry into cultured mammalian cells. These proteins also have extensive sequence similarities. Previously, Southern hybridization predicted the existence of an internalin mul
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9. The Sortase SrtA of Listeria monocytogenes Is Involved in Processing of Internalin and in Virulence
Listeria monocytogenes is an intracellular gram-positive human pathogen that invades eucaryotic cells. Among the surface-exposed proteins playing a role in this invasive process, internalin belongs to the family of LPXTG proteins, which are known to be covalently linked to the bacterial cell wall in gram-positive bacteria. Recently, it has been shown in Stap
American Society for Microbiology.
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10. Listeriosis in the Pregnant Guinea Pig: a Model of Vertical Transmission
Feto-placental infections represent a major cause of pregnancy complications, and yet the underlying molecular and cellular mechanisms of vertical transmission are poorly understood. Listeria monocytogenes, a facultative intracellular pathogen, is one of a group of pathogens that are known to cause feto-placental infections in humans and other mammals. The p
American Society for Microbiology.
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11. Identification and purification of novel internalin-related proteins in Listeria monocytogenes and Listeria ivanovii.
Monoclonal antibodies were generated against a 30-kDa protein fraction derived from culture supernatants of a Listeria monocytogenes strain complemented with additional copies of the prfA regulator gene. Several of the antibodies reacted specifically with a hitherto unidentified, secreted 30-kDa polypeptide. By immunoblot analysis, the expression of this 30k
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12. A framework for interpreting the leucine-rich repeats of the Listeria internalins
The surface protein InlB of the bacterial pathogen Listeria monocytogenes is required for inducing phagocytosis in various nonphagocytic mammalian cell types in vitro. InlB causes tyrosine phosphorylation of host cell adaptor proteins, activation of phosphoinositide 3-kinase, and rearrangements of the actin cytoskeleton. These events lead to phagocytic
The National Academy of Sciences.