Intermediate Filaments
Mostrando 13-24 de 276 artigos, teses e dissertações.
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13. Localization of bovine brain filament antibody on intermediate (100 A) filaments in guinea pig vascular endothelial cells and chick cardiac muscle cells.
Guinea pig vascular endothelial cells contain naturally occurring rings of intermediate filaments that completely encircle the nucleus. Indirect immunofluorescence staining showed that these perinuclear rings bound antibody prepared against protein from bovine brain 9-nm filaments. In endothelial cells grown in the presence of 1 muM demecolcine (Colcemid) th
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14. Different intermediate-sized filaments distinguished by immunofluorescence microscopy.
The major protein of intermediate-sized filaments in mouse 3T3 cells, for which the name vimentin is proposed, has a molecular weight of 57,000. Antibodies against vimentin and antibodies against prekeratin have been used in parallel in immunofluorescence microscopy on a variety of cultured cells as well as on frozen tissue sections. Both antibodies decorate
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15. Morphological distinction between filaments that converge upon desmosomes and those that are attached to hemidesmosomes in the epidermis of anuran larvae and lampreys.
Two populations of morphologically distinct intermediate filaments which are segregated into different compartments of the cytoplasm and which may attach to different junctional specialisations were observed in the basal layer cells of the epidermis of tadpoles of Rana catesbeiana, Rana temporaria, Bufo bufo, Leptodactylus flavopictus and Pseudis paradoxus a
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16. Functional changes of intermediate filaments in fibroblastic cells revealed by a monoclonal antibody.
We describe reversible changes of intermediate filaments of fibroblastic cells associated with changes in the functional state of the cells. The changes are revealed by comparing the immunofluorescence patterns given by a monoclonal antibody and a polyclonal serum, both recognizing vimentin. The state of the filaments depends on culture density; this effect
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17. Intermediate filaments in smooth muscle tumours
Antisera to the intermediate filaments vimentin and desmin react with fixed paraffin embedded tissue. Benign uterine myomas contain both classes of filaments. Gastrointestinal “smooth muscle tumours” however often lack desmin even when they appear histologically benign. In the sarcomas examined vimentin was the only class of intermediate filament present
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18. Immunoglobulin M autoantibody to vimentin intermediate filaments.
Serum from a patient with the CREST Syndrome and systemic lupus erythematosus contained an IgM antibody that reacted at dilutions up to 1:800 with a fibrous cytoplasmic network in several epithelioid and fibroblastic cell lines. The antibody was shown by immunofluorescence microscopy to label a specific subset of cytoskeletal polymers, the intermediate filam
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19. Intermediate Filaments in Motion: Observations of Intermediate Filaments in Cells Using Green Fluorescent Protein-VimentinV⃞
The American Society for Cell Biology.
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20. Intermediate filaments in malignant melanomas. Identification and use as marker in surgical pathology.
Intermediate-sized filaments have been studied in human malignant melanomas and in normal melanocytes by immunofluorescence microscopy with antibodies directed against keratin, vimentin, desmin, neurofilament protein, and glial filament protein. Both human melanotic and amelanotic tumor cells and tumor metastases as well as normal melanocytes in human skin a
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21. Association of microtubules and intermediate filaments in normal fibroblasts and its disruption upon transformation by a temperature-sensitive mutant of Rous sarcoma virus.
By double indirect immunofluorescence, using primary rabbit antibodies to tubulin and guinea pig antibodies to vimentin, we have simultaneously labeled microtubules and intermediate filaments in several types of cultured normal fibroblasts. With well-spread interphase cells there was an extensive but not complete correspondence of the labeling patterns for t
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22. Mitochondria are associated with microtubules and not with intermediate filaments in cultured fibroblasts.
Triple-immunofluorescence experiments with antibodies to cytochrome c oxidase, tubulin, and vimentin have been used to immunolabel the mitochondria, microtubules, and intermediate filaments inside the same cultured fibroblasts. In particular, fibroblasts were immunolabeled after they had either been transformed by infection with Rous sarcoma virus or given l
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23. Fc-mediated binding of IgG to vimentin-type intermediate filaments in vascular endothelial cells.
Prior studies have shown that vascular endothelial cells bind circulating IgG intracellularly during cell death. We now demonstrate that all endothelial cells have intracellular binding sites for IgG and that these binding sites are exposed to circulating IgG only if the plasma membrane is damaged. The binding sites are located on the cytoskeletal intermedia
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24. Structure of fibroblastic intermediate filaments: analysis of scanning transmission electron microscopy.
The structure of fibroblastic intermediate filaments from Chinese hamster ovary cells has been investigated by scanning transmission electron microscopy. Freshly extracted (native) filaments were compared with filaments reassembled in vitro from purified decamin. From digital micrographs of unstained specimens, direct measurements of linear mass density were