Insertional Mutagenesis
Mostrando 1-12 de 430 artigos, teses e dissertações.
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1. Preparation and characterization of non-viral gene delivery systems with pEGFP-C1 Plasmid DNA
ABSTRACT In recent years, non-viral delivery systems for plasmid DNA have become particularly important. They can overcome the disadvantages of viral systems such as insertional mutagenesis and unpredicted immunogenicity. Some additional advantages of non-viral gene delivery systems are; good stability, low cost, targetability, delivery of a high amount of g
Braz. J. Pharm. Sci.. Publicado em: 07/06/2018
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2. Progress with schistosome transgenesis
Genome sequences for Schistosoma japonicum and Schistosoma mansoni are now available. The schistosome genome encodes ~13,000 protein encoding genes for which the function of only a minority is understood. There is a valuable role for transgenesis in functional genomic investigations of these new schistosome gene sequences. In gain-of-function approaches, tra
Memórias do Instituto Oswaldo Cruz. Publicado em: 2011-11
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3. Overexpression of kermit/dGIPC is associated with lethality in Drosophila melanogaster
Insertional mutagenesis is an important tool for functional genomics in Drosophila melanogaster. The insertion site in the KG00562 mutant fly line has been mapped to the CG8709 (herein named DmLpin) locus and to the 3’ of kermit (also called dGIPC). This mutant line presents a high lethality rate resulting from a gain of function. To obtain some insight in
Brazilian Journal of Medical and Biological Research. Publicado em: 2011-04
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4. Beyond retrovirus infection : HIV meets gene therapy
The human immunodeficiency virus (HIV) is classified as a retrovirus because of its RNA genome and the fact that it requires reverse transcriptase to convert it into DNA. This virus belongs to the lentivirinae subfamily and is able to infect quiescent cells but is better known for its association with acquired immunodeficiency syndrome (AIDS) and can be desc
Publicado em: 2010
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5. Fatores que interferem na produção de bovicina HC5 e obtenção de mutantes não produtores de bacteriocina / Factors that affect bovicin HC5 production and isolation of nonbacteriocin producing mutants
Neste trabalho objetivou-se analisar os fatores que interferem na produção de bovicina HC5 por Streptococcus bovis HC5 e obter mutantes não produtores de bacteriocina. S. bovis HC5 cresceu e produziu bovicina HC5 utilizando várias fontes de carbono e nitrogênio. A maior atividade específica de bovicina HC5 (AU ml-1 mg de massa seca celular-1) foi obtid
Publicado em: 2009
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6. Beyond retrovirus infection: HIV meets gene therapy
The human immunodeficiency virus (HIV) is classified as a retrovirus because of its RNA genome and the fact that it requires reverse transcriptase to convert it into DNA. This virus belongs to the lentivirinae subfamily and is able to infect quiescent cells but is better known for its association with acquired immunodeficiency syndrome (AIDS) and can be desc
Genetics and Molecular Biology. Publicado em: 2006
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7. A novel approach to insertional mutagenesis of Haemophilus influenzae.
Insertional mutagenesis of the Haemophilus influenzae chromosome was accomplished by a novel method employing a 2.2-kbp element, TSTE. This element, consisting of the neo gene of Tn5 flanked by Haemophilus-specific uptake sequences, was ligated to circularized chromosomal fragments before transformation into the homologous strain. Eight mutants defective in
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8. Site Preferences of Insertional Mutagenesis Agents in Arabidopsis
We have performed a comparative analysis of the insertion sites of engineered Arabidopsis (Arabidopsis thaliana) insertional mutagenesis vectors that are based on the maize (Zea mays) transposable elements and Agrobacterium T-DNA. The transposon-based agents show marked preference for high GC content, whereas the T-DNA-based agents show preference for low GC
American Society of Plant Biologists.
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9. Insertional mutagenesis of Drosophila heterochromatin with single P elements.
Insertional mutagenesis with transposable P elements has greatly facilitated the identification and analysis of genes located throughout the 70% of the Drosophila melanogaster genome classified as euchromatin. In contrast, genetically marked P elements have only rarely been shown to transpose into heterochromatin. By carrying out single P element insertional
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10. Insertional mutagenesis and illegitimate recombination in mycobacteria.
Mycobacteria, particularly Mycobacterium tuberculosis, Mycobacterium leprae, and Mycobacterium avium, are major pathogens of man. Although insertional mutagenesis has been an invaluable genetic tool for analyzing the mechanisms of microbial pathogenesis, it has not yet been possible to apply it to the mycobacteria. To overcome intrinsic difficulties in direc
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11. Meiotic chromosome segregation mutants identified by insertional mutagenesis of fission yeast Schizosaccharomyces pombe; tandem-repeat, single-site integrations
Identification of genes required for segregation of chromosomes in meiosis (scm) is difficult because in most organisms high-fidelity chromosome segregation is essential to produce viable meiotic products. The biology of fission yeast Schizosaccharomyces pombe facilitates identification of such genes. Insertional mutagenesis was achieved by electroporation o
Oxford University Press.
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12. Identification, sequencing, and targeted mutagenesis of a DNA polymerase gene required for the extreme radioresistance of Deinococcus radiodurans.
Deinococcus radiodurans and other species of the same genus share extreme resistance to ionizing radiation and many other agents that damage DNA. Two different DNA damage-sensitive strains generated by chemical mutagenesis were found to be defective in a gene that has extended DNA and protein sequence homology with polA of Escherichia coli. Both mutant strai