Insect Cell Expression System
Mostrando 1-12 de 85 artigos, teses e dissertações.
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1. Solubility as a limiting factor for expression of hepatitis A virus proteins in insect cell-baculovirus system
The use of recombinant proteins may represent an alternative model to inactivated vaccines against hepatitis A virus (HAV). The present study aimed to express the VP1 protein of HAV in baculovirus expression vector system (BEVS). The VP1 was expressed intracellularly with molecular mass of 35 kDa. The VP1 was detected both in the soluble fraction and in the
Mem. Inst. Oswaldo Cruz. Publicado em: 11/07/2016
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2. Expressão de proteína antiviral de lonomia obliqua em sistema baculovírus/célula de inseto / Expression of an antiviral protein from Lonomia obliqua in baculovirus/insect cell system
A tecnologia de cultivo de celulas animais tem permitido nos ultimos anos o desenvolvimento de inumeros bioprodutos. Principalmente com interesse farmacologico, alguns desses produtos, as proteinas recombinantes, podem ser produzidas em sistemas de expressao heterologos em escala comercial. Bacterias, leveduras, celulas de mamiferos e de insetos sao alguns d
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 16/02/2012
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3. Estudos das interações da septina 4 humana / Study of Human Septin 4 interactions
Septinas são proteínas ligantes a GTP encontradas desde fungos até metazoários. A primeira função identificada para septinas foi o seu papel central na organização e dinâmica do septo de divisão de leveduras. Uma das características marcantes é que septinas se organizam em heterofilamentos de 7 a 9 nm de espessura que foram purificados de diverso
Publicado em: 2009
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4. Expressão da proteína do envelope do vírus da Febre Amarela em células de inseto
Yellow fever is an haemorrhagic disease caused by a virus that belongs to the genus Flavivirus (Flaviviridae family) and is transmitted by mosquitoes. It is a positive- sense, single-stranded, enveloped RNA virus, and its genome consists of 10,862 nucleotides coding for a single ORF of 10,233 nucleotides. This ORF encodes three structural proteins (capsid, p
Publicado em: 2007
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5. Aspectos moleculares da imunidade de Lutzomyia longipalpis (Díptera:Psychodidae). / Molecular aspects of immunity of Lutzomyia longipalpis (Díptera: Psychodidae).
Sandflies are known for the transmission of leishmaniasis but can also transmit viral and bacterial diseases. Also, sandflies get in contact with several insect pathogens like fungi and helminths. The strategies used by sandflies for defense against pathogens are mostly unknown. The main infection path is through blood feeding and the midgut is the first imm
Publicado em: 2007
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6. Amplificação, clonagem e expressão de proteína recombinante do vírus da doença de Aujeszky em sistema de baculovírus para utilização em programa de controle e erradicação / Amplification, cloning and expression of the recombinant protein of the Aujesskys disease vírus in baculovirus system for use in control and eradication program
Aujeszkys disease (AD) is an infect-contagious illness that causes serious economical damages to the producer and the swine industry. Aiming to develop mechanisms and to improve technologies that are faster, more sensitive and more specific for diagnosis and for use in free areas or in ADs eradication programs, the sequence codifier of the glycoprotein E (gE
Publicado em: 2006
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7. Expression of a glycosylphosphatidylinositol-anchored Trypanosoma brucei transferrin-binding protein complex in insect cells.
The expression site-associated gene ESAG 6 was previously implicated in transferrin binding in the protozoan parasite Trypanosoma brucei. ESAG 6 and the closely related ESAG 7 of T. brucei strain AnTat1.3 have now been expressed in insect cells using the baculovirus expression system. Expression of ESAG 6 alone in insect cells gives rise to a glycosylated pr
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8. Authentic processing and targeting of active maize auxin-binding protein in the baculovirus expression system.
The major auxin-binding protein (ABP1) from maize (Zea mays L.) has been expressed in insect cells using the baculovirus expression system. The recombinant protein can be readily detected in total insect cell lysates by Coomassie blue staining on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Our data suggest that ABP1 is processed sim
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9. Functional expression of the alpha 2 and alpha 3 isoforms of the Na,K-ATPase in baculovirus-infected insect cells.
Multiple isoforms of both the alpha and beta subunits of Na,K-ATPase have been identified. Elucidating their roles has been complicated by the fact that most tissues express multiple isoforms and purification techniques specific for each isoform have not been achieved. The baculovirus expression system, which uses the baculovirus Autographica californica to
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10. Identification of Epstein-Barr virus terminal protein 1 (TP1) in extracts of four lymphoid cell lines, expression in insect cells, and detection of antibodies in human sera.
The terminal proteins TP1 and TP2 are putative products of Epstein-Barr virus (EBV) genes expressed during the latent cycle of the virus. They are predicted to code for 53- and 40-kilodalton integral membrane proteins. We used the baculovirus Autographa californica nuclear polyhedrosis virus as an expression vector to produce TP1 in large amounts in insect c
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11. Glycoprotein H of herpes simplex virus type 1 requires glycoprotein L for transport to the surfaces of insect cells.
In mammalian cells, formation of heterooligomers consisting of the glycoproteins H and L (gH and gL) of herpes simplex virus type 1 is essential for the cell-to-cell spread of virions and for the penetration of virions into cells. We examined whether formation of gH1/gL1 heterooligomers and cell surface expression of the complex occurs in insect cells. Three
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12. Baculovirus-mediated expression of retinoic acid receptor type gamma in cultured insect cells reveals a difference in specific DNA-binding behavior with the 1,25-dihydroxyvitamin D3 receptor.
The baculovirus genetic expression system has been used to produce murine retinoic acid receptor (RAR) type gamma in Spodoptera frugiperda insect cells and Manduca sexta insect larvae. A hydroxyapatite binding assay revealed production levels of 300 pmol of unoccupied receptor per mg of protein in insect cells, whereas levels from infected insect larvae were