Influenza Virology
Mostrando 1-12 de 25 artigos, teses e dissertações.
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1. Genetic diversity of hemagglutinin (HA) of Influenza A virus from 1995 to 2006. / Diversidade genética da hemaglutinina (HA) de vírus influenza A, entre 1995 e 2006
Os Influenzavirus podem ser classificados de acordo com suas glicoproteínas externas hemaglutinina (HA) e neuraminidase (NA), ambas apresentando alta variabilidade genética e antigênica. No presente estudo foi realizada análise molecular do gene HA, do vírus influenza A (IA) em amostras colhidas de crianças e lactentes com sintomatologia respiratória
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 11/08/2011
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2. Epidemiologia e caracterização molecular do vírus da Influenza em quatro espécies de pinguins na Região Antártica. / Epidemiology and molecular characterization of the influenza virus in four penguin species of the antartic region.
Epidemics and pandemics of influenza usually refer to infections in human beings. The influenza virus is not, however, restricted to humans and can cause infirmity and death in other species including horses, swine, marine mammals, birds, and others. Ecological studies of viral infections have led to the hypothesis that the influenza viruses that attack mamm
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 23/02/2011
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3. Immunogenicity of the vaccine against seasonal influenza in hiv-infected and non-infected children and adolescents / Imunogenicidade da vacina contra o vírus da influenza sazonal em crianças e adolescentes infectados e não infectados pelo vírus da imunodeficiência humana
INTRODUÇÃO: Indivíduos infectados pelo HIV apresentam maior risco de quadros graves de infecção por influenza sazonal e, portanto, devem receber doses anuais da vacina contra gripe. No entanto, a capacidade dos indivíduos responderem às vacinas com títulos apropriados de anticorpos depende de variáveis como tipo de antígeno vacinal, idade e grau de
Publicado em: 2011
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4. Suboptimal Detection of Influenza Virus in Adults by the Directigen Flu A+B Enzyme Immunoassay and Correlation of Results with the Number of Antigen-Positive Cells Detected by Cytospin Immunofluorescence
To provide 24-h influenza diagnosis for adults presenting to the emergency department, the Directigen Flu A+B enzyme immunoassay (EIA) was performed in the chemistry laboratory during the night shift. Nasopharyngeal swabs were retested by cytospin-enhanced direct immunofluorescence (DFA; SimulFluor respiratory screen) when the virology laboratory opened. The
American Society for Microbiology.
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5. Isolation of influenza virus in human lung embryonated fibroblast cells (MRC-5) from clinical samples.
Ninety-four pharyngeal swab samples corresponding to 94 patients with suspected influenza virus infection were inoculated in Madin-Darby canine kidney (MDCK) cells, the conventional cell system for the isolation of influenza virus, and in fibroblastic human embryo lung (MRC-5) cells, a cell system less commonly used for this purpose but one frequently used i
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6. Reverse genetics approach towards understanding pathogenesis of H5N1 Hong Kong influenza A virus infection.
In 1990, Palese and colleagues established a method (reverse genetics) that allowed one to generate influenza virus containing a gene segment derived from cloned cDNA. Although this method contributed tremendously to our understanding of influenza pathogenesis, the requirement of helper viruses limited its use in many experimental settings. Recently, we and
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7. Characterization and evaluation of monoclonal antibodies developed for typing influenza A and influenza B viruses.
Monoclonal antibodies that are broadly reactive with influenza A or influenza B viruses were produced as stable reagents for typing influenza viruses. Monoclonal antibodies to influenza A were specific for either matrix protein or nucleoprotein. The antibodies to influenza B were specific for nucleoprotein or hemagglutinin protein. In an enzyme immunoassay p
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8. Mapping of the influenza virus genome. III. Identification of genes coding for nucleoprotein, membrane protein, and nonstructural protein.
In previous communications we reported that the eight RNA segments of influenza A/PR/8/34 (HON1) virus could be distinguished from corresponding segments of influenza A/Hong Kong/8/68 (H3N2) virus by migration on polyacrylamide-urea gels. Examination of the RNA patterns of the two parent viruses and recombinants derived from them in concert with serological
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9. Caspase-Dependent N-Terminal Cleavage of Influenza Virus Nucleocapsid Protein in Infected Cells
The nucleocapsid protein (NP) (56 kDa) of human influenza A viruses is cleaved in infected cells into a 53-kDa form. Likewise, influenza B virus NP (64 kDa) is cleaved into a 55-kDa protein with a 62-kDa intermediate (O. P. Zhirnov and A. G. Bukrinskaya, Virology 109:174–179, 1981). We show now that an antibody specific for the N terminus of influenza A vi
American Society for Microbiology.
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10. Influenza type A virus neuraminidase does not play a role in viral entry, replication, assembly, or budding.
We have used a neuraminidase-deficient influenza virus, NWS-Mvi, which was selected by supplying bacterial neuraminidase in the medium (C. Liu and G. M. Air, Virology 194:403-407, 1993), to define the role of neuraminidase in influenza virus replication. Electron microscopy showed that virions of the NWS-Mvi mutant assembled normally and formed large aggrega
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11. Molecular mechanism underlying the action of a novel fusion inhibitor of influenza A virus.
In the initial stages of influenza virus infection, the hemagglutinin (HA) protein of influenza virus mediates both adsorption and penetration of the virus into the host cell. Recently, we identified and characterized BMY-27709 as an inhibitor of the H1 and H2 subtypes of influenza A virus that specifically inhibits the HA function necessary for virus-cell m
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12. Segment 8 of the influenza virus genome is unique in coding for two polypeptides.
In previous studies we showed that a ninth polypeptide with a molecular weight of approximately 11,000 (NS2) found in influenza virus-infected cells was unique, that it could be synthesized in vitro, and that its expression in vivo required early protein synthesis. On the basis of these results we suggested that one of the eight genome RNA segments of influe