Inducible Promotor
Mostrando 1-12 de 13 artigos, teses e dissertações.
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1. Avaliação da tolerância ao estresse salino de plantas de cana-de-açúcar transformadas com o gene P5CS
A salinidade pode interferir na produção de cana-de-açúcar de duas maneiras: afetando o crescimento e o rendimento, como também reduzindo a concentração de sacarose no colmo. Plantas submetidas ao estresse salino podem acumular compostos orgânicos não tóxicos de baixo peso molecular, coletivamente conhecido como solutos ou osmólitos compatíveis,
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 19/03/2010
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2. Toll-like receptor 4 (TLR-4) and inducible nitric oxide synthase (iNOS) gene polymorphisms are associated with type 2 diabetes / Investigação de polimorfismos em genes do sistema imune inato em uma população com diabetes tipo 2
Atualmente, o DM2 constitui-se como um grande problema de saúde pública mundial devido à sua elevada prevalência, morbimortalidade, além do impacto que apresenta nos custos públicos com a saúde. Os mecanismos fisiopatológicos desta doença têm se tornado cada vez mais evidentes, estando relacionados com o envolvimento exclusivo de células, receptor
Publicado em: 2009
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3. Sweet orange (Citrus sinensis L. Osbeck) genetic transformation with the hrpN gene (harpin) and evaluation for citrus canker (Xanthomonas axonopodis pv. citri) resistance / Transformação genética de laranja doce (Citrus sinensis L. Osbeck) com o gene hrpN (harpina) e avaliação da resistência ao cancro cítrico (Xanthomonas axonopodis pv. citri)
The citrus industry has a great economic and social importance for Brazil, which is considered largest producer and orange juice exporter in the world. However, serious problems related to pathogens have affected citrus production and quality. With the development of genetic engineering and the characterization of genes related with plant disease resistance,
Publicado em: 2007
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4. Induction of tumor promotor-inducible genes in murine 3T3 cell lines and tetradecanoyl phorbol acetate-nonproliferative 3T3 variants can occur through protein kinase C-dependent and -independent pathways.
We isolated a group of genes that are rapidly and transiently induced in 3T3 cells by tetradecanoyl phorbol acetate (TPA). These genes are called TIS genes (for TPA-inducible sequences). Epidermal growth factor (EGF), fibroblast growth factor (FGF), and TPA activated TIS gene expression with similar induction kinetics. TPA pretreatment to deplete protein kin
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5. Nuclease-hypersensitive sites in chromatin of the estrogen-inducible apoVLDL II gene of chicken.
DNAseI-hypersensitive sites were localized in apoVLDL II chromatin from chicken. In the liver two sites at 1.75 and 1.0 kb upstream from the cap-site are present before the gene is activated. After induction by estradiol a number of additional sites appear, three in the promotor region of the gene, one within the coding region and two behind the poly-A signa
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6. Genetic Control of the 2-Keto-3-Deoxy-d-Gluconate Metabolism in Escherichia coli K-12: kdg Regulon
2-Keto-3-deoxy-gluconate (KDG), an intermediate of the hexuronate pathway in Escherichia coli K-12, is utilized as the sole carbon source only in strains derepressed for the specific KDG-uptake system. KDG is metabolized to pyruvate and glyceraldehyde-3-phosphate via the inducible enzymes KDG-kinase and 2-keto-3-deoxy-6-phosphate-gluconate (KDPG) aldolase. H
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7. Identification of an E1A-inducible cellular factor that interacts with regulatory sequences within the adenovirus E4 promoter.
We have previously shown that E1A-mediated induction of the adenovirus E2 transcription unit likely involves the posttranslational activation of a previously limiting cellular factor termed E2F. However, this factor is not involved in E1A induction of several other viral genes, including the E4 gene, since it does not bind to the promoters of these genes. We
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8. Isolation of ara-lac gene fusions in Salmonella typhimurium LT2 by using transducing bacteriophage Mu d (Apr lac).
A specialized Mu transducing phage containing a gene encoding ampicillin resistance and the lac structural genes without the lac promotor [Mu d(apr lac)] has been constructed and used to create gene fusions in Escherichia coli (M. J. Cadadaban and S. N. Cohen, Proc. Natl. Acad. Sci. U.S.A. 76:4530--4533, 1979). Transposition of the Mu d(Apr lac) phage to chr
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9. VE-PTP and VE-cadherin ectodomains interact to facilitate regulation of phosphorylation and cell contacts
VE-cadherin is the essential adhesion molecule in endothelial adherens junctions, and the regulation of protein tyrosine phosphorylation is thought to be important for the control of adherens junction integrity. We show here that VE-PTP (vascular endothelial protein tyrosine phosphatase), an endothelial receptor-type phosphatase, co-precipitates with VE-cadh
Oxford University Press.
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10. A recombinant human Fab expressed in Escherichia coli neutralizes rabies virus.
A recombinant human anti-rabies monoclonal antibody (MAb-57) Fab was prepared by cloning the heavy (Fd)- and light-chain domains into the same bacterial expression vector. To construct the recombinant Fab, mRNA was extracted from MAb-57-producing hybridoma cells, reverse transcribed, and then amplified by polymerase chain reaction (PCR) by using oligonucleot
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11. Molecular dissection of the mouse interleukin-4 promoter.
Understanding the molecular mechanisms regulating the expression of interleukin 4 (IL-4) may shed light on the differentiation of lymphokine-producing phenotypes of CD4+ T cells. We have identified two DNA segments that are necessary for full phorbol 12-myristate 13-acetate (PMA)-induced activity of the IL-4 promoter region in the thymoma cell line EL4. Thro
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12. Net (ERP/SAP2) one of the Ras-inducible TCFs, has a novel inhibitory domain with resemblance to the helix-loop-helix motif.
The three ternary complex factors (TCFs), Net (ERP/ SAP-2), ELK-1 and SAP-1, are highly related ets oncogene family members that participate in the response of the cell to Ras and growth signals. Understanding the different roles of these factors will provide insights into how the signals result in coordinate regulation of the cell. We show that Net inhibits