Immunoglobulin Light Chains
Mostrando 1-12 de 281 artigos, teses e dissertações.
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1. Serum free light chain assays not total light chain assays are the standard of care to assess Monoclonal Gammopathies
Abstract The diagnosis of Multiple Myeloma is a challenge to the physician due to the non-specific symptoms (anemia, bone pain and recurrent infections) that are commonplace in the elderly population. However, early diagnosis is associated with less severe disease, including fewer patients presenting with acute renal injury, pathological fractures and severe
Rev. Bras. Hematol. Hemoter.. Publicado em: 2016-02
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2. Serum levels of immunoglobulin free light chains in patients with chronic hepatitis C presenting cryoglobulinemia
Hepatitis C virus (HCV) infects B-lymphocytes, provokes cellular dysfunction and causes lymphoproliferative diseases such as cryoglobulinemia and non-Hodgkin's B-cell lymphoma. In the present study, we investigated the serum levels of kappa and lambda free light chains (FLC) of immunoglobulins and the kappa/lambda FLC ratio in Brazilian patients with chronic
Braz J Infect Dis. Publicado em: 2014-12
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3. Urine screening by Seldi-Tof, followed by biomarker identification, in a Brazilian cohort of patients with Renal Cell Carcinoma (RCC)
Purpose To screen proteins/peptides in urine of Renal Cell Carcinoma (RCC) patients by SELDI-TOF (Surface Enhanced Laser Desorption Ionization - Time of Flight) in search of possible biomarkers. Material and Methods Sixty-one urines samples from Clear Cell RCC and Papillary RCC were compared to 29 samples of control urine on CM10 chip. Mass analysis was p
Int. braz j urol.. Publicado em: 2013-04
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4. Estratégias para expressão de um anticorpo anti-CD3 humanizado em células de mamífero
The development of new pharmaceuticals derived from recombinant antibodies had a significant impact in the treatment of various illnesses. However, due to the structural complexity of the immunoglobulin molecule, the production of these molecules in mammal cells is still challenging. One of the difficulties is the equimolar production of heavy and light chai
Publicado em: 2009
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5. Detection of immunoglobulin light and heavy chain genes rearrangements in cutaneous B cell lymphoproliferative infiltrates / Pesquisa do rearranjo dos genes das cadeias leve e pesada de imunoglobulina nos processos linfoproliferativos cutâneos de células B
INTRODUÇÃO: O diagnóstico diferencial dos processos linfoproliferativos cutâneos de célula B permanece um desafio para patologistas, dermatologistas, hematologistas e oncologistas, apesar dos recentes avanços imunoistoquímicos e moleculares. OBJETIVO: Este trabalho avaliou o auxílio diagnóstico e as limitações da pesquisa da clonalidade utilizando
Publicado em: 2007
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6. Comparison of different monoclonal antibodies against immunosuppressive proteins of Ascaris suum
The extract of Ascaris suum suppresses the humoral and cellular immune responses to unrelated antigens in the mouse. In order to further characterize the suppressive components of A. suum, we produced specific monoclonal antibodies which can provide an important tool for the identification of these proteins. The A. suum immunosuppressive fractions isolated b
Brazilian Journal of Medical and Biological Research. Publicado em: 2004-02
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7. Nephrotoxicity of Bence-Jones proteins: interference in renal epithelial cell acidification
The aim of the present study was to evaluate the acidification of the endosome-lysosome system of renal epithelial cells after endocytosis of two human immunoglobulin lambda light chains (Bence-Jones proteins, BJP) obtained from patients with multiple myeloma. Renal epithelial cell handling of two BJP (neutral and acidic BJP) was evaluated by rhodamine fluor
Brazilian Journal of Medical and Biological Research. Publicado em: 2002-03
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8. Pontes dissulfeto inter cadeia de uma paraproteina M(JJO) IgG1 Glm(zax)
Plasma from a patient (JJO) with multiple myeloma showing high levels of M paraprotein was obtained from the faculdade de Medicina, Universidade Estadual de Campinas.The results of electrophoretic analysis followed by densitometry showed a compact band indicative of molecular homogeneity characteristic of patient presenting monoclonal gamopathy. Fraction A c
Publicado em: 1983
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9. A hereditary immunoglobulin A abnormality: absence of light-heavy—chain assembly: Study of immunoglobulin synthesis in tonsillar cells
A new immunoglobulin A abnormality, absence of assembly of α-chain and light-chain, was found in an adult female suffering from recurrent upper respiratory infection and tonsillitis since childhood, but otherwise healthy. The IgA abnormality was manifest in her serum by the presence of free α-chains, in her saliva by the presence of α-chains bound to secr
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10. Pattern of Immunoglobulin Synthesis and Assembly in a Human-Mouse Somatic Cell Hybrid Clone
Fusion of human peripheral blood lymphocytes, not forming detectable immunoglobulins, with mouse myeloma cells (TEPC-15), secreting mouse immunoglobulin A with known antibody activity, yielded a somatic cell hybrid clone that secreted both human and mouse immunoglobulins. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that hu
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11. Rate of Somatic Mutation in Immunoglobulin Production by Mouse Myeloma Cells
Cultures of mouse myeloma cells which secrete both heavy (H) and light (L) immunoglobulin chains were cloned in soft agar. Variants that synthesized neither chain, or only light chains, were detected by overlaying the growing colonies with antiserum specific for the individual immunoglobulin chains. The rate of conversion of heavy plus light chain producers
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12. N-Terminal Amino-Acid Sequence of African Lungfish Immunoglobulin Light Chains
The sequence of the N-terminal ten amino acids of the unblocked light chains derived from the low molecular weight immunoglobulin of a dipnoid fish, the African lungfish (Protopterus aethiopicus), has been determined. A degree of sequence heterogeneity as extensive as that displayed by pooled mammalian light chains was encountered. The major N-terminal seque