Immunofluorescent Antibodies
Mostrando 13-24 de 225 artigos, teses e dissertações.
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13. Development of enzyme-linked immunosorbent assays based on recombinant MSP1a and MSP2 of Anaplasma marginale
Indirect enzyme-linked immunosorbent assays (ELISAs) based on recombinant MSP1a and MSP2 from a Brazilian isolate of Anaplasma marginale were developed to detect antibodies against this rickettsia in cattle. The high sensitivities (99% for both tests) and specificities (100% for both tests) were confirmed with sera from cattle positive or negative for A. mar
Memórias do Instituto Oswaldo Cruz. Publicado em: 2005-11
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14. Variants of the Plasmodium vivax circumsporozoite protein (VK210 and VK247) in Colombian isolates
Phenotypic diversity has been described in the central repeated region of the circumsporozoite protein (CSP) from Plasmodium vivax. Two sequences VK210 (common) and VK247 (variant) have been found widely distributed in P. vivax isolates from several malaria endemic areas around the world. A third protein variant called P. vivax-like showing a sequence simila
Memórias do Instituto Oswaldo Cruz. Publicado em: 2001-07
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15. Virus da lingua azul : estudo do antigeno viral, produzido a partir do soro tipo 4, para fins de diagnostico sorologico
In the present study a strain of Bluetongue virus, serotype 4 (BTV-S4), when adapted to BHK 21, clone 13 and to VERO cell lines has shown a distinctive cytophatic effect between 72 h and 96 h after inoculation, with titres ranging from 10 POT. 3.6 to 10 POT. 3.6 DICT 50%/ml. After a high speed centrifugation of the packed infected cells the viral particles w
Publicado em: 1990
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16. Characterisation of immunofluorescent heterophile antibodies which may be confused with autoantibodies.
Approximately 6% of sera submitted for routine immunofluorescent autoantibody screeening produced characteristic reaction patterns against a variety of animal and human tissues. It is suggested that these non-tissue-specific patterns represent a complex family of heterophile antibodies which could be confused with certain autoantibodies. It is further sugges
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17. Preparation of polyvalent viral immunofluorescent intracellular antigens and use in human serosurveys.
A method is described for preparation of polyvalent antigens for use in rapid screening for immunofluorescent antibodies to Lassa, Marburg, and Ebola viruses. The technique uses mixtures of specifically infected Vero cells placed on Teflon-templated microscopy slides. It was found to be as sensitive as the use of monovalent antigens for detection and quantit
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18. Antibodies to the ring-infected erythrocyte surface antigen of Plasmodium falciparum elicited by infection with Plasmodium malariae.
The ring-infected erythrocyte surface antigen (RESA) of Plasmodium falciparum (RESA-P), found in the membrane of erythrocytes infected with young asexual stages of P. falciparum, is a promising vaccine candidate. Antibodies to RESA-P were inducible by infection with another human malaria species, P. malariae. Of 298 serum samples from inhabitants of three is
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19. Affinity-purified antibodies to ring-infected erythrocyte surface antigen do not correlate with merozoite invasion inhibition in Plasmodium falciparum.
We affinity purified, from malaria-immune serum, antibody to the ring-infected erythrocyte surface antigen (RESA), using petri dishes containing a monolayer of Plasmodium falciparum ring-infected erythrocytes. Except for one out of eight samples, the purified antibody positive by RESA-immunofluorescent assay was not inhibitory to the in vitro invasion of mer
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20. Assessment of virus infectivity by the immunofluorescent and immunoperoxidase techniques.
The immunofluorescent and immunoperoxidase cell-counting techniques were comparable in precision and reproducibility for the quantitative assessment of influenza virus infectivity. The dose-response function was linear with each procedure, and comparable results were obtained for estimating neutralizing antibodies in antiviral serum.
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21. Erythrocyte antinuclear antibodies in sera of chickens hyperimmunized with group A streptococcal vaccine.
Chickens hyperimmunized with group A streptococcal vaccine often synthesize high levels of antibody to group A streptococcal carbohydrate (SACHO). Indirect immunofluorescent analysis revealed that sera of these chickens also contain antinuclear antibodies capable of reacting with chicken erythrocyte nuclei (EANA) at titers up to 2,560. Removal of the anti-SA
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22. Systemic Lupus Erythematosus. STUDIES OF THE ANTIBODIES BOUND TO SKIN
Systemic lupus erythematosus is characterized by antibodies demonstrable by immunofluorescence on the renal glomeruli and at the basement membrane area of both normal and involved skin. Acid eluates from glomeruli and from normal-appearing skin of three patients with systemic lupus erythematosus contained an antinuclear antibody. This antibody fixes compleme
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23. Age-related antibodies to rotavirus in New Zealand rabbits.
The prevalence of antibodies to rotavirus was determined in 199 New Zealand rabbits from a commercial rabbitry by an indirect immunofluorescent test. Virtually all rabbits less than 1 month old and greater than 2 months old had antibodies, whereas only 25% of rabbits 1 to 2 months old had antibodies.
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24. Diethylaminoethyl-cellulose-bacterial cell immunoadsorbent columns: preparation of serotype-specific globulin and immunofluorescent conjugates for Streptococcus mutans serotypes a and d.
Diethylaminoethyl (DEAE)-cellulose was used as a support material for preparing bacterial cell columns. Pretreatment of the bacterial cells with formalin was essential in obtaining satisfactory adherence of the cells to DEAE-cellulose. Cross-reacting antibodies were removed from antibody preparations against strains of Streptococcus mutans serotypes a and d