Immortalization
Mostrando 1-12 de 349 artigos, teses e dissertações.
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1. Skp2 inhibitor SKPin C1 decreased viability and proliferation of multiple myeloma cells and induced apoptosis
Multiple myeloma (MM) is a malignant neoplasm of plasma, and exhibits several harmful effects including osteolytic injuries, hypercalcemia, and immune dysfunction. Many patients with MM succumb to the underlying malignancy. An S-phase kinase-related protein 2 (Skp2) inhibitor, designated SKPin C1, has been developed and confirmed to have an inhibitory effect
Braz J Med Biol Res. Publicado em: 25/04/2019
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2. Telomerase reverse transcriptase mediated immortalization of human bone marrow stromal cells
Primary human bone marrow stromal cells (hMSCs) were transfected with human telomerase reverse transcriptase (hTERT) gene with lipofection method. The hTERT transfected hMSCs of passage 100 underwent chondrogenesis induction with dexamethasone, transforming the growth factor β and vitamin C, osteogenesis induction with dexamethasone, β glycerophosphoric ac
Braz. arch. biol. technol.. Publicado em: 2014-02
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3. Epstein-Barr virus nuclear antigen-2 detection and typing in immunocompromised children correlated with lymphoproliferative disorder biopsy findings
Epstein-Barr virus (EBV), the causative agent of infectious mononucleosis, plays a significant role as a cofactor in the process of tumorigenesis, and has consistently been associated with a variety of malignancies especially in immunocompromised patients. Forty-four children and adolescents (21 liver transplant patients, 7 heart transplant, 5 AIDS, 3 autoim
Brazilian Journal of Infectious Diseases. Publicado em: 2008-06
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4. Conventional cytogenetic characterization of a new cell line, ACP01, established from a primary human gastric tumor
Gastric cancer is the second most frequent type of neoplasia and also the second most important cause of death in the world. Virtually all the established cell lines of gastric neoplasia were developed in Asian countries, and western countries have contributed very little to this area. In the present study we describe the establishment of the cell line ACP01
Brazilian Journal of Medical and Biological Research. Publicado em: 2004-12
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5. Shabat e a conservaÃÃo do judaÃsmo. O sÃtimo dia faz renascer a quintessÃncia e a partÃcula elementar do universo
This study deals with the Jewish ritual of the Sabbath here seen as a passing ritual from the profane to the sacred. Sabbath, which in Hebrew means to cease, corresponds to the seventh day, the Saturday. Since in the Jewish calendar the day begins in the end of the afternoon, the Sabbath has its start in the Fridayâs sunset, during until the Saturdayâs sun
Publicado em: 2003
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6. Efeito do fator de necrose tumoral-alfa (TNF-α)sobre células imortalizadas por papilomavírus humano (HPV) / Effects of tumor necrosis factor-alpha (TNF-α) on HPV - immortalized cells
A infecção por papilomavírus humano (HPV) é o principal fator de risco para o desenvolvimento de neoplasias intra-epiteliais cervicais, as lesões precursoras do carcinoma da cérvice uterina. O fator de necrose tumoral-α (TNF-α) é um dos principais mediadores da inflamação da pele e das mucosas. Esta citocina é capaz de inibir a prolifera�
Publicado em: 2002
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7. The viral transcriptional regulatory region upstream of the E6 and E7 genes is a major determinant of the differential immortalization activities of human papillomavirus types 16 and 18.
The human papillomavirus types 16 (HPV-16) and 18 (HPV-18) can immortalize primary human keratinocytes. The region of the viral genome responsible for this function maps to the E6 and E7 genes and their respective upstream transcriptional regulatory sequences, the long control regions (LCRs). The HPV-18 LCR/E6/E7 is more efficient in this immortalization fun
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8. Functional implications of mutations within polyomavirus large T antigen Rb-binding domain: effects on pRb and p107 binding in vitro and immortalization activity in vivo.
In this study, we have extensively modified the Rb-binding domain of polyomavirus large T antigen. Mutant polyomavirus large T antigens were tested for their ability to bind pRb and p107 in vitro and assayed for their capacity to immortalize primary rat embryo fibroblasts in vivo. Polyomavirus large T antigen bound pRb and p107 through a common region locate
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9. Association of p53 binding and immortalization of primary C57BL/6 mouse embryo fibroblasts by using simian virus 40 T-antigen mutants bearing internal overlapping deletion mutations.
To more precisely map the immortalization and p53 binding domains of T antigen, a large series of overlapping deletion mutations were created between codons 251 to 651 by utilizing a combination of Bal 31 deletion and oligonucleotide-directed mutagenesis. Immortalization assay results indicated that amino acids (aa) 252 to 350, 400, and 451 to 532 could be r
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10. Cyclin D1 overexpression and p53 inactivation immortalize primary oral keratinocytes by a telomerase-independent mechanism
The immortalization of human cells is a critical step in multistep carcinogenesis. Oral-esophageal carcinomas, a model system to investigate molecular mechanisms underlying squamous carcinogenesis, frequently involve cyclin D1 overexpression and inactivation of the p53 tumor suppressor. Therefore, our goal was to establish the functional role of cyclin D1 ov
American Society for Clinical Investigation.
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11. Immortalization of human foreskin keratinocytes by various human papillomavirus DNAs corresponds to their association with cervical carcinoma.
Normal human foreskin keratinocytes cotransfected with the neomycin resistance gene and recombinant human papillomavirus (HPV) DNAs (types 16, 18, 31, and 33) that have a high or moderate association with cervical malignancy acquired immortality and contained integrated and transcriptionally active viral genomes. Only transcripts from the intact E6 and E7 ge
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12. Degradation of p53, Not Telomerase Activation, by E6 Is Required for Bypass of Crisis and Immortalization by Human Papillomavirus Type 16 E6/E7
Bypass of two arrest points is essential in the process of cellular immortalization, one of the components of the transformation process. Expression of human papillomavirus type 16 E6 and E7 together can escape both senescence and crisis, processes which normally limit the proliferative capacity of primary human keratinocytes. Crisis is thought to be mediate
American Society for Microbiology.