Hypotonic Stress
Mostrando 1-12 de 21 artigos, teses e dissertações.
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1. Regulatory volume decrease in Leishmania mexicana: effect of anti-microtubule drugs
The trypanosomatid cytoskeleton is responsible for the parasite's shape and it is modulated throughout the different stages of the parasite's life cycle. When parasites are exposed to media with reduced osmolarity, they initially swell, but subsequently undergo compensatory shrinking referred to as regulatory volume decrease (RVD). We studied the effects of
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-02
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2. Influência de combinações de diferentes caotrópicos no espectro visível da hemoglobina e na estabilidade de membrana de eritrócitos humanos / Influence of combinations of different chaotropes in the visible spectrum of hemoglobin
CHAPTER II: This study aimed to investigate the influence of combinations of urea, ethanol and hypotonicity on the stability and spectrum of human hemoglobin. Protocols with fixed incubation time of 30 minutes at 37 C were used to analyze the spectra of commercial hemoglobin and hemoglobin of venous blood obtained from 20 healthy volunteers. The visible spec
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 19/01/2011
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3. Effects of glycerol on the thermal dependence of the stability of human erythrocytes / Efeitos do glicerol sobre a dependência térmica da estabilidade de eritrócitos humanos
The incubation of human erythrocytes with ethanol in saline physiologic solution, by 30 minutes, can produce lysis of the expanded morphological state (R), which presents a lower stability, or formation of the contracted morphological state (T), which has a higher stability. The R state occurs in physiological conditions and suffers lysis under less elevated
Publicado em: 2007
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4. Tyrosine kinase activation is an immediate and essential step in hypotonic cell swelling-induced ERK activation and c-fos gene expression in cardiac myocytes.
Hypotonic stress causes rapid cell swelling and initiates various cellular adaptive processes. However, it is unknown how cells initially sense low osmolarity and convert it into intracellular signals. We investigated the signal transduction mechanism initiated by hypotonic cell swelling in cardiac myocytes using c-fos expression as a nuclear marker. Treatme
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5. Microtitre plate measurement of platelet response to hypotonic stress.
The conventional method of assessing the platelet response to hypotonic stress (HSR) was adapted to allow microtitre plate technology to be used. After water is added to a platelet suspension two sequential readings are taken at 414 nM on a vertical microplate reader. The difference between the second (three minutes) and the first (one minute) was defined as
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6. Effects of repetitive tetanic stimulation at long intervals on excitation-contraction coupling in frog skeletal muscle.
1. Single skeletal muscle fibres of Xenopus frogs were used to investigate the possibility that excitation-contraction (E-C) coupling can be impaired under conditions of elevated intracellular free Ca2+ ([Ca2+]i). 2. Fibres were stimulated with a train of up to 200 tetani at 10 or 20s intervals; this long-interval stimulation (LIS) scheme was chosen to minim
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7. Volume-dependent K+ and Cl- fluxes in rat thymocytes.
1. Hypotonic stress unmasked inward and outward K+ and Cl- movements in rat thymocytes. This KCl flux stimulation was reduced by DIOA (dihydroindenyl-oxy-alkanoic acid), but not by DIDS (4,4'-diisothiocyanostilbene-2,2'-disulphonate), quinidine, DPAC 144 (5-nitro-2-(2-phenylethyl-amino)-benzoic acid), bumetanide or ouabain. 2. In isotonic media (308 +/- 5 mo
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8. Pannexin 1 Contributes to ATP Release in Airway Epithelia
ATP is a paracrine regulator of critical airway epithelial cell functions, but the mechanism of its release is poorly understood. Pannexin (Panx) proteins, related to invertebrate innexins, form channels (called pannexons) that are able to release ATP from several cell types. Thus, ATP release via pannexons was examined in airway epithelial cells. Quantitati
American Thoracic Society.
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9. Calcium-Independent Activation of Salicylic Acid-Induced Protein Kinase and a 40-Kilodalton Protein Kinase by Hyperosmotic Stress1
Reversible protein phosphorylation/dephosphorylation plays important roles in signaling the plant adaptive responses to salinity/drought stresses. Two protein kinases with molecular masses of 48 and 40 kD are activated in tobacco cells exposed to NaCl. The 48-kD protein kinase was identified as SIPK (salicylic acid-induced protein kinase), a member of the to
American Society of Plant Physiologists.
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10. Three different Cl- channels in the bovine ciliary epithelium activated by hypotonic stress.
1. Hypotonic solution induced transient Cl- channel activity in both pigmented and non-pigmented ciliary epithelial cells in cell-attached patches. 2. The activation time constants for these currents were 63 and 97 s for non-pigmented and pigmented ciliary epithelial (NPCE and PCE) cells, respectively. The currents inactivated during the exposure to hypotoni
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11. Autocrine signaling through ATP release represents a novel mechanism for cell volume regulation.
Recovery of cell volume in response to osmotic stress is mediated in part by increases in the Cl- permeability of the plasma membrane. These studies evaluate the hypothesis that ATP release and autocrine stimulation of purinergic (P2) receptors couple increases in cell volume to opening of Cl- channels. In HTC rat hepatoma cells, swelling induced by hypotoni
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12. Vacuole Fusion Regulated by Protein Phosphatase 2C in Fission Yeast
The gene ptc4+ encodes one of four type 2C protein phosphatases (PP2C) in the fission yeast Schizosaccharomyces pombe. Deletion of ptc4+ is not lethal; however, Δptc4 cells grow slowly in defined minimal medium and undergo premature growth arrest in response to nitrogen starvation. Interestingly, Δptc4 cells are unable to fuse vacuoles in response to hypot
The American Society for Cell Biology.