Hypoosmotic Test
Mostrando 1-12 de 14 artigos, teses e dissertações.
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1. Cryopreservation of semen from goats fed a diet supplemented with flaxseed
RESUMO O objetivo com o estudo foi avaliar o efeito da inclusão de semente de linhaça na dieta de machos caprinos sobre a resistência do sêmen frente ao processo de criopreservação. Foram utilizados 16 machos, os quais foram distribuídos em quatro grupos e receberam dieta suplementada com 0, 4, 8 e 12% de semente de linhaça, durante um período de 60
Rev. bras. saúde prod. anim.. Publicado em: 20/12/2019
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2. Effect of glycerol, n, n-dimethylformamide and n-methyl-2-pyrrolidone on rabbit sperm stored at 4 °C and 16 °C
Abstract Artificial insemination with cooled semen is the most common practice in rabbit farms and any improvement on it helps to increase the efficiency and productivity of rabbit meat farms. Therefore, the aim of this study was to assess whether different cryoprotectant agents (CPA) as glycerol, N, N-Dimethylformamide (DMF) and N-Methyl-2-Pyrrolidone (NMP)
Anim. Reprod.. Publicado em: 14/11/2019
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3. Seasonal variation in the reproductive activity of male goats raised under tropical climate conditions
ABSTRACT This study analyzed seasonal variations in the testes, the concentration of sex hormones, the parameters of fresh and thawed semen, and the sexual behavior of male Alpine goats from a temperate region in a tropical climate and possible interference with fertility. The maximum and minimum temperature and luminosity were recorded daily, while seminal,
R. Bras. Zootec.. Publicado em: 2017-03
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4. Comportamento sexual, parâmetros seminais e diluição pós-congelamento de sêmen de jumentos (Equus asinus) da raça Pêga / Sexual behavior, seminal parameters and dilution after freezing Pêga breed donkeys (Equus asinus) semen
The aim of this study was to elucidate aspects of asinine behavior from the description of various collections using an artificial vagina and relate this behavior with the seminal parameters. We evaluated also the availability of the formulation of the diluent T2-94 plus dimethylformamide in the protection of sperm cells during cryopreservation of semen from
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 07/03/2012
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5. Viability and fertility of cooled equine semen diluted with skimmed milk or glycine egg yolk-based extenders
Two semen extenders were compared for their ability to maintain viability of horse semen during 24 hours of cold preservation, and for the pregnancy rate after artificial insemination. In the experiment 1, five ejaculates from three stallions were split-diluted in either a skimmed milk-based extender (Kenney extender) or a glycine egg yolk-based extender (Fo
R. Bras. Zootec.. Publicado em: 2012-12
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6. Rapid thawing human sperm does not affect basic parameters in normozoospermic men: a double-blind prospective study
PURPOSE: To compare sperm recovery from slow versus rapid thawing technique using thirty-eight normozoospermic human sperm samples, as follows. Twenty-one samples from men taking part in routine infertility screening exams (infertile group) and seventeen from proven fertile volunteer men with at least one child (fertile group). MATERIALS AND METHODS: After a
International braz j urol. Publicado em: 2012-02
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7. Cigarette smoking impairs sperm bioenergetics
OBJECTIVE: The growing consensus on the negative impact of cigarette smoking on fertility prompted us to compare the rate of sperm respiration in smokers and non-smokers. MATERIALS AND METHODS: Semen samples from 20 smokers and 58 non-smokers consulting at the andrology laboratory for fertility evaluation were used. Smoking was defined as consumption of at l
International braz j urol. Publicado em: 2010-02
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8. Viabilidade in vitro de espermatozóides ovinos criopreservados em meios contendo lipoproteínas de baixa densidade nas formas natural ou liofilizada
The aim of the present work was to evaluate the efficiency of the addition of natural and lyophilized low density lipoproteins (LDL) instead of egg yolk to ram semen extender during cryopreservation. Two ejaculates from 10 rams were collected and sperm motility, mass motility, vigor, number, reactivity to the hypoosmotic swelling test and morphology were eva
Publicado em: 2009
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9. Evaluation of acrosomal status and sperm viability in fresh and cryopreserved specimens by the use of fluorescent peanut agglutinin lectin in conjunction with hypo-osmotic swelling test
OBJECTIVE: In this study, we evaluated whether the hypo-osmotic swelling test (HOST) can be used as a vital marker in combination with peanut agglutinin (PNA) - labeling in fresh and cryopreserved spermatozoa. MATERIALS AND METHODS: Human sperm populations were exposed to a hypo-osmotic medium for 60 minutes, and then incubated in a 1 µg/mL solution of the
International braz j urol. Publicado em: 2007-06
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10. Avaliação da integridade da membrana plasmática e cromatina no semen bovino congelado / Assessment of sperm plasma membrane and chromatin integrity in frozen-thawed bull semen
O objetivo da presente pesquisa foi avaliar se testes laboratoriais, usados na identificação das lesões estruturais e funcionais da membrana plasmática e da integridade da cromatina, são efetivos para explicar as variações na taxa de gestação em programas de inseminação artificial. Foram usados os dados de campo da inseminação artificial de 2.03
Publicado em: 2007
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11. Avaliação "in vitro" da adição fracionada da dimetilformamida na criopreservação de sêmen equino
The aim of this research was to evaluate the efficacy of the manner of the cryoprotectant addition, one or multiple steps, and the cooling rate, on equine semen cryopreservation. The lesion intensity of the spermatozoa, during the process of the cryopreservation, was also studied. One ejaculate of nine stallions was used to test the modified INRA 82 with 5%
Publicado em: 2005
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12. Efeito de dieta suplementada com Ãleo de girassol sobre o perfil lipÃdico plasmÃtico e seminal de caprinos
In the semi-arid region of northeast Brazil, the production of caprine is a major economical activity, especially due to the resistance and adaptation ability shown by these animals under semi-arid climate conditions. This work aims to investigate the effect of sunflower oil supplementation on plasma and semen lipid profiles, in order to evaluate whether it
Publicado em: 2005