Hydrophobic Adsorption
Mostrando 13-24 de 110 artigos, teses e dissertações.
-
13. Recuperação e purificação de proteínas do soro de queijo tipo coalho usando cromatografia de troca iônica e interação hidrofóbica em leito na forma expandida
Expanded Bed Adsorption plays an important role in the downstream processing mainly for reducing costs as well as steps besides could handling cells homogenates or fermentation broth. In this work Expanded Bed Adsorption was used to recover and purify whey proteins from coalho cheese manufacture using Streamline DEAE and Streamline SP both ionic resins as we
Publicado em: 2010
-
14. Efeito de características estruturais da quitosana sobre sua interação com filmes de Langmuir como modelo de biomembrana / Effects from the structural characteristics of chitosan on its interaction in Langmuir films as biomembrane models.
Chitosans are polysaccharides used in medicine, pharmacy, dentistry and in the inhibition of microorganisms growth (eg. as bactericidal agent). In these applications their action should depend on the interaction with cell membranes, which is difficult to verify because isolating a membrane is not easy. An alternative is to investigate the interaction with me
Publicado em: 2010
-
15. Study of partition of ovomucoid protein in aqueous twophase systems and anionic or hydrophobic resins / Estudo da partição da proteína ovomucoide em sistemas aquosos bifásicos e em resinas aniônica ou hidrofóbica
In this work, the application of the liquid-liquid extraction technique with aqueous two-phase systems (ATPS) was evaluated for the separation of ovomucoid, an egg white protein. This technique is an advisable purification process applied to large scale since it provides a selective partition with high yields. The ATPS were composed by PEG 1500 and an inorga
Publicado em: 2009
-
16. Development of membrane biosensors and characterization of the interactions between cytochrome c and hybrid bilayer membranes by Surface Plasmon Resonance / Desenvolvimento de biosensores de membranas e caracterização da interação entre citocromo c e bicamadas híbridas por ressonância plasmônica de superfície
The aim of this work was to develop membrane biosensors based on Surface Plasmon Resonance (SPR) and to apply them to study the interactions between cytochrome c (cyt c) and model membranes. SPR is an optical technique that provides high-sensitivity measurements of refractive index (n), allowing the characterization of the adsorption and desorption of molecu
Publicado em: 2008
-
17. Cellulose acetate propionate coated titanium: characterization and biotechnological application
Surfaces of pure titanium and Ti coated with cellulose acetate propionate (CAP) have been characterized by means of scanning electron microscopy X ray coupled with elemental microanalysis (SEM-EDS), ellipsometry, atomic force microscopy (AFM) and contact angle measurements. Coating Ti surfaces with CAP ultrathin films reduced original surface roughness. Surf
Materials Research. Publicado em: 2007-12
-
18. Adsorção de glicomacropeptídeo por interação hidrofóbica em leito expandido / Adsorption of the glicomacropeptide by hydrophobic interaction in expanded bed
Neste trabalho, foi estudado o processo de adsorção da proteína glicomacropeptídeo (GMP) proveniente do soro de queijo, em cromatografia de interação hidrofóbica,variando-se as condições de temperatura e a concentração de sal. Foi usada a calorimetria de titulação para conduzir uma análise termodinâmica do processo. Obtiveram-se as curvas de r
Publicado em: 2007
-
19. Nanoparticle self-assembly using patterns formed by dewetting / Auto-organização de nanoparticulas utilizando padrões formados por transição de molhabilidade
In this work, we study an original method to promote self-assembly of gold and silica nanoparticles using patterns formed by dewetting of aqueous solutions of poly (N-isopropylacrylamide) (PNIPAM) and sodium dodecylsulfate (SDS). Dewetting is a process where the free surface of an initially stable thin liquid film becomes unstable and deforms to engender rup
Publicado em: 2007
-
20. Reduction of molecular oxygen in aqueous solutions through modifying electrodesmethodology / Redução de oxigênio molecular em soluções aquosas através da metodologia de modificação de eletrodos
This work consisted in testing the viability of investigations into the electrochemical generation and identification of free radicals involved in advanced oxidative processes. In these studies, a technique which is rarely used for the electrochemical generation and identification of free radicals at organothiole-modified electrodes, electrochemical impedanc
Publicado em: 2007
-
21. Partial purification of tightly bound mitochondrial hexokinase from maize (Zea mays L.) root membranes
In mammals, hexokinase (HK) is strategically located at the outer membrane of mitochondria bound to the porin protein. The mitochondrial HK is a crucial modulator of apoptosis and reactive oxygen species generation. In plants, these properties related to HK are unknown. In order to better understand the physiological role of non-cytosolic hexokinase (NC-HK)
Brazilian Journal of Medical and Biological Research. Publicado em: 2006-09
-
22. Hydrophobic plasma polymerized hexamethyldisilazane thin films: characterization and uses
Hexametildisilazane (HMDS) plasma polymerized thin films obtained using low frequency power supplies can be used to make adsorbent films and turn surfaces hydrophobic. The aim of this work was to verify the hydrophobicity and adsorption properties of HMDS thin films (with and without the addition of oxygen, resulting in double or single layer films) obtained
Materials Research. Publicado em: 2006-03
-
23. Preconcentration in gas or liquid phases using adsorbent thin films
The possibility of preconcentration on microchannels for organic compounds in gas or liquid phases was evaluated. Microstructures with different geometries were mechanically machined using poly(methyl methacrylate) - PMMA as substrates and some cavities were covered with cellulose. The surfaces of the microchannels were modified by plasma deposition of hydro
Materials Research. Publicado em: 2006-03
-
24. IgG purification from human plasma by membrane affinity chromatography with immobilized Cu(II) and Ni(II) : effect of quelators IDA, TREN and CM-Asp / Purificação de IgG a partir do plasma humano por cromatografia em membranas con ions Cu(II) e Ni(II) imobilizados : efeito dos agentes quelantes IDA, TREN e CM-Asp
The use of immunoglobulin G (lgG) in therapeutic applications has growing vastly and it is used in the treatment of a growing number of indications. Several chromatographic techniques have been investigated for IgG purification. In this work, we investigated the effect of the chelators iminodiacetic acid (IDA), carboxymethylaspartate acid (CM-Asp) and Tris-2
Publicado em: 2006