Hydrolases
Mostrando 25-36 de 419 artigos, teses e dissertações.
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25. Produção de hidrolases pelo fungo Dicyma pulvinata, caracterização bioquímica de uma proteinase e purificação parcial desta enzima
The fungus Dicyma pulvinata (Deuteromycotina: Hyphomycete) shows strong biocontrol potential for plant diseases, and has been found in lesions caused in plants by Microcyclus ulei (Ascomycete), the agent of the South American Leaf Blight (SALB). This disease of the rubber tree (Hevea spp.), is a limiting factor for latex production and for expansion culture
Publicado em: 2007
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26. Aplicação de triagem de alto desempenho na investigação das atividades enzimaticas e enantiosseletividades de microorganismos brasileiros / Enzymatic activities and Quick E in hydrolases screening appyng fluorescent probes
Nas últimas décadas as reações utilizando biocatalisadores tem sido amplamante aplicadas na síntese orgânica, como componentes chave de muitos processos químicos industriais, levando ao aumento na demanda por novas enzimas. A maneira mais rápida e simples de detectar enzimas é através de metodologias de triagem de alto desempenho (HTS) que permitam
Publicado em: 2007
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27. High-throughput screening in enzyme assays for epoxide hydrolases and monooxygenases activity detection using whole cell / Triagem de alto desempenho para detecção de atividade de epoxido-hidrolases e monooxigenases utilizando celulas integras
Biocatalysis is a multidisciplinary area, where new technologies are developing rapidly in order to offer unique options and solutions to different organic chemistry problems, complementing the classical synthetic chemistry. Facing the need imposed by progress in this area, this project aims at the implementation of high-throughput screening (HTS) methodolog
Publicado em: 2006
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28. Esterase profile in a pyrethroid-resistant Brazilian strain of the cattle tick Boophilus microplus (Acari, Ixodidae)
The cattle tick Boophilus microplus causes great damage in livestock and is considered one of the most important tropical ectoparasites. The traditional method of control is based on the intensive use of pesticides, however the indiscriminate use of these compounds over the years has led to the selection of resistant ticks. Hydrolases, particularly esterases
Genetics and Molecular Biology. Publicado em: 2005-12
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29. Studies on whole cell fluorescence-based screening for epoxide hydrolases and Baeyer-Villiger monoxygenases
Reações de biocatálise foram realizadas em microplacas (200 µL) visando a utilização de substratos fluorogênicos (100 µmol L-1) para prospecção rápida de epóxido hidrolases (EHs) e Baeyer-Villiger monoxigenases (BVMOs) em microrganismos (células inteiras). Um protocolo final foi alcançado para EHs, com a detecção de 3 novas fontes enzimática
Journal of the Brazilian Chemical Society. Publicado em: 2004-12
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30. Enzymatic Resolution of Ethyl 3-Hydroxy-3-Phenylpropanoate and Analogs using Hydrolases
Este trabalho contribui com o estudo da relação substrato-modelo em reações de hidrólise de ésteres beta-hidroxilados secundários e terciários. Foram utilizados um beta-hidroxiéster secundário e quatro beta-hidroxiésteres terciários, com as enzimas PCL, PLE, CRL e AOP. O melhor resultado foi obtido quando efetuou-se a reação enzimática do ést
Journal of the Brazilian Chemical Society. Publicado em: 2001-12
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31. Etofibrate but not controlled-release niacin decreases LDL cholesterol and lipoprotein (a) in type IIb dyslipidemic subjects
Etofibrate is a hybrid drug which combines niacin with clofibrate. After contact with plasma hydrolases, both constituents are gradually released in a controlled-release manner. In this study, we compared the effects of etofibrate and controlled-release niacin on lipid profile and plasma lipoprotein (a) (Lp(a)) levels of patients with triglyceride levels of
Brazilian Journal of Medical and Biological Research. Publicado em: 2001-02
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32. Purificação, caracterização, clonagem e seqüenciamento de β-glicosidades de Tenebrio molitor (Coleoptera) / Purification, characterization, cloning and sequencing of β-glycosidases from Tenebrio molitor (Coleoptera)
In the midgut lumen of Tenebrio molitor larvae there are 4 β-glycosidases (named 1, 2, 3a and 3b), not present in the animal food. They were purified with electrophoresis, ion exchange and hydrophobic chromatographies. β-glycosidase 1 (relative molecular weight - Mr 59,000) is unstable at 30°C but is stabilized by substrates. The enzyme hydrolyses
Publicado em: 2001
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33. Hidrolases da parede celular em sementes de Euphorbia heterophylla L. durante a germinação e desenvolvimento inicial da plântula. / Cell wall hydrolases in the seeds of Euphorbia heterophylla L. during germination and early seedling development.
Na maioria das sementes a emergência da radícula caracteriza o término da germinação e marca o início do desenvolvimento da plântula. A atividade das hidrolases da parede celular durante a pré-emergência pode estar associada ao amolecimento do tecido que circunda o embrião, principalmente na região micropilar, onde ocorre a protrusão da radícula
Publicado em: 2001
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34. Mitogenic effect of lysosomal hydrolases on bovine tracheal myocytes in culture.
Studies were conducted to assess the mitogenic effect of lysosomal hydrolases, enzymes known to have an association with allergen- or ozone-induced airway hyperreactivity, on bovine tracheal myocytes in culture. Addition of purified human placental beta-hexosaminidase and partially purified bovine liver beta-glucuronidase resulted in the doubling of cell cou
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35. Lysosomal hydrolases of different classes are abnormally distributed in brains of patients with Alzheimer disease.
beta-Amyloid formation requires multiple abnormal proteolytic cleavages of amyloid precursor protein (APP), including one within its intramembrane domain. Lysosomes, which contain a wide variety of proteases (cathepsins) and other acid hydrolases, are major sites for the turnover of membrane proteins and other cell constituents. Using immunocytochemistry, im
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36. Effects of Multiple Deletions of Murein Hydrolases on Viability, Septum Cleavage, and Sensitivity to Large Toxic Molecules in Escherichia coli
The multiplicity of murein hydrolases found in most bacteria presents an obstacle to demonstrating the necessity of these potentially autolytic enzymes. Therefore, Escherichia coli mutants with deletions in multiple murein hydrolases, including lytic transglycosylases, amidases, and dd-endopeptidases, were constructed. Even a mutant from which seven differen
American Society for Microbiology.