Hybridoma
Mostrando 1-12 de 607 artigos, teses e dissertações.
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1. Advances and challenges in therapeutic monoclonal antibodies drug development
The use of serum containing polyclonal antibodies from animals immunized with toxins marked the beginning of the application of antibody-based therapy in late nineteenth century. Advances in basic research led to the development of the hybridoma technology in 1975. Eleven years later, the first therapeutic monoclonal antibody (mAb) was approved, and since th
Braz. J. Pharm. Sci.. Publicado em: 08/11/2018
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2. Preparation of monoclonal antibodies against gamma-type phospholipase A2 inhibitors and immunodetection of these proteins in snake blood
Abstract Background: The gamma-type phospholipase A2 inhibitor (ΡLIγ) is a natural protein commonly found in snake serum, which can neutralize pathophysiological effects of snake venom phospholipases A2. Therefore, this protein is a potential candidate to the development of a novel antivenom. To the best of our knowledge, there is no antibody currently av
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 28/09/2017
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3. Produção e caracterização da porção Fab do anticorpo anti-digoxina utilizando a tecnologia de phage display. / Production and characterization of the Fab portion of anti-digoxin antibody by phage display technology.
Digoxin is a pharmaceutical used in the control of cardiac dysfunction. Its therapeutic window is very narrow. To counteract the toxic effect, polyclonal anti-digoxin Fab fragments are commercially available. Our goal was to obtain variants of monoclonal anti-digoxin Fab fragments by phage display technology, which allows the generation of high affinity and
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 27/03/2012
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4. Development of two potential diagnostic monoclonal antibodies against human cytomegalovirus glycoprotein B
Human cytomegalovirus glycoprotein B (gB) represents a target for diagnosis and treatment in view of the role it plays in virus entry and spread. Nevertheless, to our knowledge, rare detection of a gB antigen has been reported in transplant patients and limited information is available about diagnostic gB monoclonal antibodies (mAbs). Our aim was to develop
Brazilian Journal of Medical and Biological Research. Publicado em: 2012-07
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5. Anticorpo monoclonal de alta eficiência no desenvolvimento de imunoquímica aplicada : análise de ocratoxina em vinho
O agronegócio globalizado, cada vez mais competitivo, exige segurança com enfoque na legislação sanitária perante alimento saudável aos consumidores. No cenário, o controle de Ocratoxina A (OTA) em produtos derivados de uva, com ênfase ao vinho, já é uma realidade na Comunidade Européia, cujo monitoramento contínuo deve ser executado empregando m
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 13/12/2010
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6. Produção de hibridomas e clones produtores de anticorpos monoclonais anti- Nucleosídeo trifosfato difosfohidrolase 1 (NTPDase-1) recombinante de Trypanosoma cruzi / Production of hybridomas and clones producing monoclonal anti nucleoside triphosphate diphosphohydrolase-1 (NTPDase-1) recombinant Trypanosoma cruzi
O protozoário Trypanosoma cruzi, é o agente etiológico da doença de Chagas. As ecto-nucleotidases da família E-NTPDases são enzimas capazes de hidrolisar nucleotídeos tri e/ou difosfatados nos seus produtos monofosfatados. A NTPDase-1 de T. cruzi é uma proteína presente na superfície do mesmo, com possibilidade de também ser secretada para o meio
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 02/09/2010
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7. Comparative study of the Fc region from murine IgG1 anaphylactic and non anaphylactic antibodies / Estudo comparativo da região Fc de anticorpos IgG1 murinos anafiláticos e não-anafiláticos
It is well established that the glycosylation process is essential for the structural conformation and effector function of the antibodies. However, it is quite clear how differences in the carbohydrates attached to the antibodies may interfere with their biological activities. It was previously reported that murine IgG1 antibodies can be divided into anaphy
Publicado em: 2010
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8. Aplicação biotecnológica : anticorpo monoclonal anti-desoxinivalenol para monitoramento e avaliação da exposição pelo consumo de trigo (Triticum aestivum L.)
Fusarium spp., fitopatógeno relevante no campo, pode produzir micotoxinas de ocorrência inevitável em produtos de origem vegetal. F. graminearum contamina trigo e pode produzir desoxinivalenol (DON), tricotoceno de caráter citotóxico, emético e inibidor de síntese protéica, responsável por perdas na agricultura e produção animal. Analítica sem us
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/10/2009
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9. Papel dos receptores do tipo Toll na morte celular induzida por ativação (AICD) de linfócitos T. / Role of Toll-Like receptors in the activation-induced cell death (AICD) of T cells hybridoma.
During an infection the number of T lymphocytes increases dramatically. The clonal expansion phase is followed by reducing the level of activated T cells that depends, in part, a process of cell death induced by activation (AICD). Our group showed that when APCs are stimulated with LPS, a TLR4 agonist, produce PGE2, which inhibits the AICD of CD4 + T cells b
Publicado em: 2009
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10. Development of immunoassay techniques to detect microcystin in environmental samples / Desenvolvimento de técnicas de imunoensaio para detecção de microcistina em amostras ambientais
The contamination of drinking water by cyanobacterial toxins is a public health issue and a concern for water authorities throughout the world. Microcystin-LR (MCLR) is a hazardous cyclic heptapeptide cyanotoxin, which inhibits protein phosphatase PP1 and PP2A in hepatocytes. Microcystins are produced by several genera of cyanobacteria and presents more than
Publicado em: 2009
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11. Caracterização metabólica e cinética do cultivo de três hibridomas para produção de imunoglobulinas com especificidade e antígenos eritrocitários para uso hemoterápico.
In the last two decades mammalian cell cultures have been broadly used for the industrial production of pharmaceutical, proteins, in particular the monoclonal antibodies by cultures of murine hybrid cells (hybridoma). The development of bioprocess for industrial scale of monoclonal antibodys (MAbs) depends on the determination and optimization of several phy
Publicado em: 2007
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12. Anticorpo monoclonal anti-AFB1 : coluna de imunoafinidade e espectrofluorimetria para controle de qualidade de alimentos
The frequent occurrence of aflatoxin in foodstuffs causes a potential negative impact on Brazilian agribusiness. Aflatoxin has been classified in group 1 by International Association in Research on Cancer. The production of immunoreagents associated with immunochemical assay developments could allow accessible and low cost quality control aiming prevention o
Publicado em: 2007