Hybridisation
Mostrando 1-12 de 685 artigos, teses e dissertações.
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1. Lutzomyia longipalpis (Diptera: Psychodidae) Argentina-Bolivia border: new report and genetic diversity
American visceral leishmaniasis (AVL) has two main scenarios of transmission as follows: scattered cases in rural areas and urban outbreaks. Urban AVL is in active dispersion from the northeastern border of Argentina-Paraguay-Brazil to the South. The presence of Lutzomyia longipalpis was initially reported in urban environments in the northwestern border of
Mem. Inst. Oswaldo Cruz. Publicado em: 30/09/2019
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2. Mycobacterium tuberculosis promotes genomic instability in macrophages
BACKGROUND Mycobacterium tuberculosis is an intracellular pathogen, which may either block cellular defensive mechanisms and survive inside the host cell or induce cell death. Several studies are still exploring the mechanisms involved in these processes. OBJECTIVES To evaluate the genomic instability of M. tuberculosis-infected macrophages and compare it
Mem. Inst. Oswaldo Cruz. Publicado em: 2018-03
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3. Use of fluorescent oligonucleotide probes for differentiation between Paracoccidioides brasiliensis and Paracoccidioides lutzii in yeast and mycelial phase
BACKGROUND Fluorescence in situ hybridisation (FISH) associated with Tyramide Signal Amplification (TSA) using oligonucleotides labeled with non-radioactive fluorophores is a promising technique for detection and differentiation of fungal species in environmental or clinical samples, being suitable for microorganisms which are difficult or even impossible
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-02
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4. Hoteles y sanatorios: influencia de la tuberculosis en la arquitectura del turismo de masas
Abstract The aim of this article is to verify the influence of tuberculosis on mass tourism architecture. To achieve this, the paper analyses the typological evolution of the hotel since the middle of the nineteenth century, when the first sanatoria appeared in the alpine resorts. A study that creates links between the progress in architecture and the medica
Hist. cienc. saude-Manguinhos. Publicado em: 2017-01
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5. First description of Leishmania (Viannia) infection in Evandromyia saulensis, Pressatia sp. and Trichophoromyia auraensis (Psychodidae: Phlebotominae) in a transmission area of cutaneous leishmaniasis in Acre state, Amazon Basin, Brazil
Studies on the sandfly fauna to evaluate natural infection indexes are still limited in the Brazilian Amazon, a region with an increasing incidence of cutaneous leishmaniasis. Here, by using a multiplex polymerase chain reaction directed to Leishmania kDNA and hybridisation, we were able to identify L. (Viannia) subgenus in 12 out of 173 sandflies captured i
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-01
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6. Genetic engineering of cotton with a novel cry2AX1 gene to impart insect resistance against Helicoverpa armigera
Abstract Embryogenic calli of cotton (Coker310) were cocultivated with the Agrobacterium tumefaciens strain LBA4404 harbouring the codon-optimised, chimeric cry2AX1 gene consisting of sequences from cry2Aa and cry2Ac genes isolated from Indian strains of Bacillus thuringiensis. Forty-eight putative transgenic plants were regenerated, and PCR analysis of thes
Crop Breed. Appl. Biotechnol.. Publicado em: 2016-09
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7. A molecular platform for the diagnosis of multidrug-resistant and pre-extensively drug-resistant tuberculosis based on single nucleotide polymorphism mutations present in Colombian isolates of Mycobacterium tuberculosis
Developing a fast, inexpensive, and specific test that reflects the mutations present in Mycobacterium tuberculosis isolates according to geographic region is the main challenge for drug-resistant tuberculosis (TB) control. The objective of this study was to develop a molecular platform to make a rapid diagnosis of multidrug-resistant (MDR) and extensively d
Mem. Inst. Oswaldo Cruz. Publicado em: 02/02/2016
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8. Evolution of Trypanosoma cruzi: clarifying hybridisations, mitochondrial introgressions and phylogenetic relationships between major lineages
Several different models of Trypanosoma cruzi evolution have been proposed. These models suggest that scarce events of genetic exchange occurred during the evolutionary history of this parasite. In addition, the debate has focused on the existence of one or two hybridisation events during the evolution of T. cruzi lineages. Here, we reviewed the literature a
Mem. Inst. Oswaldo Cruz. Publicado em: 24/03/2015
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9. The first report of the vanC1 gene in Enterococcus faecium isolated from a human clinical specimen
The vanC1 gene, which is chromosomally located, confers resistance to vancomycin and serves as a species marker for Enterococcus gallinarum. Enterococcus faecium TJ4031 was isolated from a blood culture and harbours the vanC1gene. Polymerase chain reaction (PCR) assays were performed to detect vanXYc and vanTc genes. Only the vanXYc gene was found in the E.
Mem. Inst. Oswaldo Cruz. Publicado em: 13/08/2014
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10. In house reverse membrane hybridisation assay versus GenoType MTBDRplus and their performance to detect mutations in the genes rpoB, katG and inhA
Drug-resistant tuberculosis (TB) threatens global TB control and is a major public health concern in several countries. We therefore developed a multiplex assay (LINE-TB/MDR) that is able to identify the most frequent mutations related to rifampicin (RMP) and isoniazid (INH) resistance. The assay is based on multiplex polymerase chain reaction, membrane hybr
Mem. Inst. Oswaldo Cruz. Publicado em: 07/05/2014
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11. Optimisation of an asymmetric polymerase chain reaction assay for the amplification of single-stranded DNA from Wuchereria bancrofti for electrochemical detection
Single-stranded DNA (ssDNA) is a prerequisite for electrochemical sensor-based detection of parasite DNA and other diagnostic applications. To achieve this detection, an asymmetric polymerase chain reaction method was optimised. This method facilitates amplification of ssDNA from the human lymphatic filarial parasite Wuchereria bancrofti. This procedure prod
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-09
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12. Chromosomal divergence and evolutionary inferences in Rhodniini based on the chromosomal location of ribosomal genes
In this study, we used fluorescence in situ hybridisation to determine the chromosomal location of 45S rDNA clusters in 10 species of the tribe Rhodniini (Hemiptera: Reduviidae: Triatominae). The results showed striking inter and intraspecific variability, with the location of the rDNA clusters restricted to sex chromosomes with two patterns: either on one
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-05