Hybrid Repair
Mostrando 13-24 de 152 artigos, teses e dissertações.
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13. Estudo do comportamento mecânico de dutos de aço com reparos compósitos hibridos / Mechanical behavior of steel pipes repaired with a hybrid laminate
Worldwide, steel pipes play an important role in many industrial sectors. Meanwhile, any kind of duct is prone to suffer superficial damages due to corrosion. In this sense, techniques which allow fast and reliable repairs of ducts have a fundamental importance to increase the life cycle, avoid accidents, minimize environmental contaminations and to keep the
Publicado em: 2010
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14. Estudo estrutural e funcional das proteínas PilZ e YaeQ do fitopatógeno Xanthomonas axonopodis pv citri / Structural and functional studies of PilZ and YaeQ from Xanthomonas axonopodis pv citri proteins
The aim of the project was to perform structural and functional studies of different Xanthomonas axonopodis pv citri (Xac) proteins including the hypothetical proteins YaeQ and SufE; RpfC, RpfF and RpfG involved in the quorum sensing and PilZ, FimX and PilB that play roles in type IV pilus (T4P) biogenesis. Several experimental techniques were employed inclu
Publicado em: 2010
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15. Analise funcional e estrutural da proteina HBx do virus da Hepatite B / Functional and structural analysis of the hepatitis B virus X protein
Chronic infection of the hepatitis B virus (HBV) is one of the causes leading to liver cancer. The HBV genome encodes the 17 kDa onco-protein HBx, a multi-functional protein that contains 10 cysteine residues and is related to induce liver cancer in transgenic mice. The exact function of HBx is still unknown. However, it has been shown that HBx is a potent t
Publicado em: 2005
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16. Repair-deficient xeroderma pigmentosum cells made UV light resistant by fusion with X-ray-inactivated Chinese hamster cells.
Xeroderma pigmentosum (XP) is an autosomal recessive human disease, characterized by an extreme sensitivity to sunlight, caused by the inability of cells to repair UV light-induced damage to DNA. Cell fusion was used to transfer fragments of Chinese hamster ovary (CHO) chromosomes into XP cells. The hybrid cells exhibited UV resistance and DNA repair charact
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17. Molecular recombination and the repair of DNA double-strand breaks in CHO cells.
Molecular recombination and the repair of DNA double-strand breaks (DSB) have been examined in the G-0 and S phase of the cell cycle using a temperature-sensitive CHO cell line to test i) if there are cell cycle restrictions on the repair of DSB's' ii) the extent to which molecular recombination can be induced between either sister chromatids or homologous c
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18. The mismatch repair system contributes to meiotic sterility in an interspecific yeast hybrid.
The mismatch repair system is the major barrier to genetic recombination during interspecific sexual conjugation in prokaryotes. The existence of this anti-recombination activity has implications for theories of evolution and the isolation of species. To determine if this phenomenon occurs in eukaryotes, the effect of a deficiency of mismatch repair on the m
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19. Physical interaction between components of DNA mismatch repair and nucleotide excision repair
Nucleotide excision repair (NER) and DNA mismatch repair are required for some common processes although the biochemical basis for this requirement is unknown. Saccharomyces cerevisiae RAD14 was identified in a two-hybrid screen using MSH2 as “bait,” and pairwise interactions between MSH2 and RAD1, RAD2, RAD3, RAD10, RAD14, and RAD25 subsequently were de
The National Academy of Sciences.
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20. Meiotic Mismatch Repair Quantified on the Basis of Segregation Patterns in Schizosaccharomyces Pombe
Hybrid DNA with mismatched base pairs is a central intermediate of meiotic recombination. Mismatch repair leads either to restoration or conversion, while failure of repair results in post-meiotic segregation (PMS). The behavior of three G to C transversions in one-factor crosses with the wild-type alleles is studied in Schizosaccharomyces pombe. They lead t
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21. Escherichia coli DNA helicase II (uvrD gene product) catalyzes the unwinding of DNA.RNA hybrids in vitro.
DNA helicase II is a well-characterized Escherichia coli enzyme capable of unwinding duplex DNA and known to be involved in both methyl-directed mismatch repair and excision repair of pyrimidine dimers. Here it is shown that this enzyme also catalyzes the ATP-dependent unwinding of a DNA.RNA hybrid consisting of a radioactively labeled RNA molecule annealed
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22. Detection of thymine [2+2] photodimer repair in DNA: selective reaction of KMnO4.
The specific reaction of potassium permanganate with thymine in single-stranded DNA was employed to analyze thymine [2+2] dimer repair in DNA and in DNA/peptide nucleic acid hybrid duplexes. This simple and highly sensitive chemical assay is convenient for monitoring repair of thymine dimers in oligonucleotides.
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23. Enzymatic reaction with unnatural substrates: DNA photolyase (Escherichia coli) recognizes and reverses thymine [2+2] dimers in the DNA strand of a DNA/PNA hybrid duplex
Peptide nucleic acids (PNA) are mimics with normal bases connected to a pseudopeptide chain that obey Watson–Crick rules to form stable duplexes with itself and natural nucleic acids. This has focused attention on PNA as therapeutic or diagnostic reagents. Duplexes formed with PNA mirror some but not all properties of DNA. One fascinating aspect of PNA bio
The National Academy of Sciences.
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24. Human chromosome 13 compensates a DNA repair defect in UV-sensitive mouse cells by mouse--human cell hybridization.
A human chromosome responsible for excision repair of UV-induced DNA damage has been identified by studying somatic cell hybrids between an UV-sensitive mutant of mouse lymphoma L5178Y cells and normal human lymphocytes. An autosomal recessive mutant, Q31, of complementation group I is deficient in excision repair of UV-induced DNA damage. Somatic cell hybri