High Throughput Nucleotide Sequencing
Mostrando 1-12 de 33 artigos, teses e dissertações.
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1. Phylogenomic and single nucleotide polymorphism analyses revealed the hybrid origin of Spondias bahiensis (family Anacardiaceae): de novo genome sequencing and comparative genomics
Abstract The genus Spondias (family Anacardiaceae) comprises 19 taxa, ten of which occur in Neotropical regions. Spondias bahiensis has been suggested to be a hybrid, although initial evidence does not support this hypothesis. The aim of this study was to test the hypothesis of the hybrid origin of S. bahiensis using high-throughput sequencing with single nu
Genet. Mol. Biol.. Publicado em: 29/11/2018
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2. Heterozygous HTRA1 missense mutation in CADASIL-like family disease
The aim of this study was to find related pathogenic genes in cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy in (CADASIL)-like patients. The direct sequencing and high-throughput multiplex polymerase chain reaction (PCR) was performed to screen for related genes. The clinical and imaging data of a CADASIL-like pati
Braz J Med Biol Res. Publicado em: 15/03/2018
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3. The complete mitochondrial genome of Engyodontium album and comparative analyses with Ascomycota mitogenomes
Abstract Engyodontium album is a widespread pathogen that causes different kinds of dermatoses and respiratory tract diseases in humans and animals. In spite of its perniciousness, the basic genetic and molecular background of this species remains poorly understood. In this study, the mitochondrial genome sequence of E. album was determined using a high-thro
Genet. Mol. Biol.. Publicado em: 23/10/2017
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4. Evaluation of MC1R high-throughput nucleotide sequencing data generated by the 1000 Genomes Project
Abstract The advent of next-generation sequencing allows simultaneous processing of several genomic regions/individuals, increasing the availability and accuracy of whole-genome data. However, these new approaches may present some errors and bias due to alignment, genotype calling, and imputation methods. Despite these flaws, data obtained by next-generation
Genet. Mol. Biol.. Publicado em: 08/05/2017
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5. The mitogenome of Onchocerca volvulus from the Brazilian Amazonia focus
We report here the first complete mitochondria genome of Onchocerca volvulus from a focus outside of Africa. An O. volvulus mitogenome from the Brazilian Amazonia focus was obtained using a combination of high-throughput and Sanger sequencing technologies. Comparisons made between this mitochondrial genome and publicly available mitochondrial sequences ident
Mem. Inst. Oswaldo Cruz. Publicado em: 2016-01
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6. High-throughput nucleotide sequence analysis of diverse bacterial communities in leachates of decomposing pig carcasses
The leachate generated by the decomposition of animal carcass has been implicated as an environmental contaminant surrounding the burial site. High-throughput nucleotide sequencing was conducted to investigate the bacterial communities in leachates from the decomposition of pig carcasses. We acquired 51,230 reads from six different samples (1, 2, 3, 4, 6 and
Genet. Mol. Biol.. Publicado em: 2015-09
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7. Estratégia para investigação molecular de epilepsia com identificação de genes relacionados a formas de polimicrogiria = : Strategy of molecular investigation on epilepsy with the identification of genes related to poymicrogyrias / Strategy of molecular investigation on epilepsy with the identification of genes related to poymicrogyrias
A polimicrogiria (PMG) é uma malformação do córtex cerebral causada por falhas no seu desenvolvimento, caracterizando-se por um número excessivo de pequenos giros e laminação anormal, dando à superfície cortical uma aparência irregular e grosseira. A gravidade de suas manifestações clínicas se relaciona diretamente com a extensão da malformaç�
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/08/2012
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8. AnÃlise in silico de novos potenciais polimorfismos genÃticos de risco na DoenÃa de Alzheimer em bancos de dados de Microarrays
Genomic and Proteomic studies of neurodegenerative disorders require complementary approaches to integrate the massive amount of data generated in high throughput experimental procedures. We propose a Bioinformatics pipeline in which expression studies guide the selection of candidate genes that should be screened for potential new genetic variations from a
Publicado em: 2009
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9. The Human MitoChip: A High-Throughput Sequencing Microarray for Mitochondrial Mutation Detection
Somatic mitochondrial mutations are common in human cancers, and can be used as a tool for early detection of cancer. We have developed a mitochondrial Custom Reseq™ microarray as an array-based sequencing platform for rapid and high-throughput analysis of mitochondrial DNA. The MitoChip contains oligonucleotide probes synthesized using standard photolitho
Cold Spring Harbor Laboratory Press.
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10. Alkaline-mediated differential interaction (AMDI): A simple automatable single-nucleotide polymorphism assay
The key requirements for high-throughput single-nucleotide polymorphism (SNP) typing of DNA samples in large-scale disease case-control studies are automatability, simplicity, and robustness, coupled with minimal cost. In this paper we describe a fluorescence technique for the detection of SNPs that have been amplified by using the amplification refract
The National Academy of Sciences.
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11. PolyPhred: automating the detection and genotyping of single nucleotide substitutions using fluorescence-based resequencing.
Fluorescence-based sequencing is playing an increasingly important role in efforts to identify DNA polymorphisms and mutations of biological and medical interest. The application of this technology in generating the reference sequence of simple and complex genomes is also driving the development of new computer programs to automate base calling (Phred), sequ
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12. Efficient High-Throughput Resequencing of Genomic DNA
Targeted resequencing of genomic DNA from organisms such as humans is an important tool enabling experimental access to variation within the species and between similar species. Taking full advantage of the reference genome sequences in designing robust, specific PCR assays and using stringent conditions, resequencing can be done efficiently without purifica
Cold Spring Harbor Laboratory Press.