High Cell Density Cultivation
Mostrando 1-10 de 10 artigos, teses e dissertações.
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1. Evaluation of the effects of sugarcane molasses as a carbon source for Ankistrodesmus gracilis and Haematococcus pluvialis (Chlorophyceae)
Resumo Duas espécies de Chlorophyceae, Ankistrodesmus gracilis e Haemotococcus pluvialis foram utilizadas para comparar o efeito do melaço de cana-de-açúcar como fonte de carbono. Nos dois meios de cultura de microalgas, a maior densidade celular foi obtida em meio de cultura comercial (CHU12 e WC). Durante o crescimento exponencial (1º ao 10º dia), de
Braz. J. Biol.. Publicado em: 21/10/2019
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2. Sistema automático de supervisão e controle de cultivos de alta densidade celular de E. coli recombinante
Cultivos de alta densidade celular de E. coli recombinante constituem uma tecnica rapida e economica para producao de proteinas recombinantes. Por meio deste bioprocesso, sao obtidos produtos de alto valor agregado e de grande importancia na industria farmaceutica, tais como insulina, hormonios de crescimento humano e bovino, antigenos proteicos para formula
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 22/12/2011
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3. Presença de dinoflagelados bentônicos potencialmente tóxicos: identificação do perigo em áreas destinadas à maricultura na Baía de Sepetiba, RJ. / Presence of potentially toxic benthic dinoflagellates: identification of hazard in shellfish growing areas in the Sepetiba Bay, RJ.
The malacoculture (cultivation of shellfish) is the second most significant activity of the Brazilian mariculture, where the cultivation of bivalve molluscs (mussels, oysters and scallops) stands out nationally. The bivalves show an excellent nutritional value when they are used as food by humans, containing a great biological value of protein, low in fat, v
Publicado em: 2009
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4. Controle hormonal da defesa à herbivoria em tomateiro / Hormonal control of herbivory defense in tomato
Even though the cultivation of tomato has a high economical importance, this culture is classified as being of elevated risk because of it common infestation by pests and diseases. This problem makes this culture greatly dependent of the application of several agrochemicals, which leads to increase in cost of production, environmental damages and also facili
Publicado em: 2009
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5. MOUSE LEUKEMIA VIRUS: “SPONTANEOUS” RELEASE BY MOUSE EMBRYO CELLS AFTER LONG-TERM in vitro CULTIVATION*
BALB/c mouse embryo cells maintained in tissue culture on a schedule of rapid transfer at high cell density develop into tumorigenic lines that have lost contact inhibition of cell division. After several months in culture, certain of these lines begin to release mouse leukemia virus. This virus has properties indistinguishable from those of virus found in a
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6. A novel pH2 control on the expression of flagella in the hyperthermophilic strictly hydrogenotrophic methanarchaeaon Methanococcus jannaschii
The methanarchaeon, Methanococcus jannaschii, a hyperthermophilic, autotrophic, and strictly hydrogenotrophic inhabitant of submarine hydrothermal vents, was cultivated in a reactor at two hydrogen partial pressure (pH2) values, 178 kPa (high) and 650 Pa (ultralow), and the cells were subjected to a comparative proteome analysis. From these studies, it
The National Academy of Sciences.
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7. Reactor-Scale Cultivation of the Hyperthermophilic Methanarchaeon Methanococcus jannaschii to High Cell Densities
For the hyperthermophilic and barophilic methanarchaeon Methanococcus jannaschii, we have developed a medium and protocols for reactor-scale cultivation that improved the final cell yield per liter from ∼0.5 to ∼7.5 g of packed wet cells (∼1.8 g dry cell mass) under autotrophic growth conditions and to ∼8.5 g of packed wet cells (∼2 g dry cell mass
American Society for Microbiology.
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8. Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli
Pure, soluble and functional proteins are of high demand in modern biotechnology. Natural protein sources rarely meet the requirements for quantity, ease of isolation or price and hence recombinant technology is often the method of choice. Recombinant cell factories are constantly employed for the production of protein preparations bound for downstream purif
BioMed Central.
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9. Stereoselective production of (+)-trans-chrysanthemic acid by a microbial esterase: cloning, nucleotide sequence, and overexpression of the esterase gene of Arthrobacter globiformis in Escherichia coli.
The gene coding for a novel esterase which stereoselectively hydrolyzes the (+)-trans (1R,3R) stereoisomer of ethyl chrysanthemate was cloned from Arthrobacter globiformis SC-6-98-28 and overexpressed in Escherichia coli. The cellular content of the active enzyme reached 33% of the total soluble protein in the recombinant E. coli JM105 cells and 5.6 g/liter
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10. Excretion of Human β-Endorphin into Culture Medium by Using Outer Membrane Protein F as a Fusion Partner in Recombinant Escherichia coli
Escherichia coli BL21 strains were found to excrete a large amount of outer membrane protein F (OmpF) into culture medium during high-cell-density cultivation. From this interesting phenomenon, a novel and efficient OmpF fusion system was developed for the excretion of recombinant proteins by E. coli. The ompF gene of E. coli BL21(DE3) was first knocked out
American Society for Microbiology.