Heteronuclear Coupling
Mostrando 1-12 de 15 artigos, teses e dissertações.
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1. Detailed 1H and 13C NMR Spectral Data Assignment for Two Dihydrobenzofuran Neolignans
In this work we present a complete proton (1H) and carbon 13 (13C) nuclear magnetic resonance (NMR) spectral analysis of two synthetic dihydrofuran neolignans (±)-trans-dehydrodicoumarate dimethyl ester and (±)-trans-dehydrodiferulate dimethyl ester. Unequivocal assignments were achieved by 1H NMR, proton decoupled 13C (13C{1H}) NMR spectra, gradient-selec
J. Braz. Chem. Soc.. Publicado em: 2016-01
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2. Estratégias de aumento de eficiência da análise de produtos naturais por espectroscopia de ressonância magnética nuclear / Strategies to improve efficiency Natural Products analys by Nuclear Magnetic Resonance Spectroscopy
Dois dos principais aspectos da Química de Produtos Naturais são a identificação e a determinação estrutural dos compostos orgânicos presentes em materiais de origem vegetal ou animal. Para realizar esta tarefa são empregados diversos métodos de separação e espectroscópicos. Dentre os métodos espectroscópicos, destaca-se a espectroscopia de res
Publicado em: 2010
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3. Determination and analysis of nJch (n=2,3,4) coupling constants in norbornane derivatives / Determinação e analise das constantes de acoplamento nJch (n=2,3,4) em derivados do norbornano
In the introduction, it is presented a discussion about the effect of hyperconjugative interactions on coupling constants and a discussion about some methodologies for the measurement of long range heteronuclear coupling constants. The reader is introduced to the topics which are the basis of this thesis. Then it is presented the results of the measurement o
Publicado em: 2009
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4. Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants.
Two heteronuclear proton-carbon NMR experiments are applied to the DNA-octamer d(TTGGCCAA)2 with carbon in natural abundance. They lead to a complete assignment of the carbon resonances of the sugars and bases. In addition, several heteronuclear coupling constants, proton-carbon as well as proton-phosphorous and phosphorous-carbon, were determined. The infor
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5. Assignment of the 13C nuclear magnetic resonance spectrum of a short DNA-duplex with 1H-detected two-dimensional heteronuclear correlation spectroscopy.
Proton-detected 1H-13C heteronuclear correlated spectroscopy [( 1H,13C]-COSY) was used to establish relations between the carbon-13 and proton nuclear magnetic resonance chemical shifts in the hexadeoxynucleoside pentaphosphate d-(GCATGC)2. Using the previously established sequence-specific proton NMR assignments, sequence-specific assignments were thus obta
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6. Secondary structure of Src homology 2 domain of c-Abl by heteronuclear NMR spectroscopy in solution.
The Src homology 2 (SH2) domain is a recognition motif thought to mediate the association of the cytoplasmic proteins involved in signal transduction by binding to phosphotyrosyl-containing sequences in proteins. Assignments of nearly all 1H and 15N resonances of the SH2 domain from the c-Abl protein-tyrosine kinase have been obtained from homonuclear and he
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7. Dual-frequency 2D-IR spectroscopy heterodyned photon echo of the peptide bond
The structure fluctuations of the peptide bond interacting with solvent are examined through the coupling and correlations of the frequency distributions of amide I and amide II transitions. The fluctuations of the two modes are anticorrelated as a result of the solvent-induced changes in the mixing of the dominant valence-bond structures of the peptide. Sig
The National Academy of Sciences.
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8. Synthesis, complete 1H assignments and conformations of the self-complementary hexadeoxyribonucleotide [d(CpGpApTpCpG)]2 and its fragments by high field NMR.
The two deoxyribonucleotides [d(CpGpApTpCpG)]2 and [d(CpGpCpG)]2 were synthesized by the phosphotriester method. Their duplex form under the conditions of the 1H-nmr experiments was proven by end 32P labeling with T4 polynucleotide kinase followed by butt end joining employing the absolute specificity of T4 ligase for double stranded DNA and analysis using g
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9. Determining RNA solution structure by segmental isotopic labeling and NMR: application to Caenorhabditis elegans spliced leader RNA 1.
Recent developments in multidimensional heteronuclear NMR spectroscopy and large-scale synthesis of uniformly 13C- and 15N-labeled oligonucleotides have greatly improved the prospects for determination of the solution structure of RNA. However, there are circumstances in which it may be advantageous to label only a segment of the entire RNA chain. For exampl
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10. Solution structure of a DNA hairpin and its disulfide cross-linked analog.
The solution structures of a 21 base long DNA hairpin derived from the ColE1 cruciform, and an analog possessing a disulfide cross-link bridging the terminal bases, have been determined by NMR spectroscopy. The 8 bp long stem of these sequences adopts a B-form helix whereas the five base long single-stranded loop appears to be flexible and cannot be represen
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11. Observation of force-detected nuclear magnetic resonance in a homogeneous field
We report the experimental realization of BOOMERANG (better observation of magnetization, enhanced resolution, and no gradient), a sensitive and general method of magnetic resonance. The prototype millimeter-scale NMR spectrometer shows signal and noise levels in agreement with the design principles. We present 1H and 19F NMR in both solid and liquid samples
National Academy of Sciences.
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12. The weak interdomain coupling observed in the 70 kDa subunit of human replication protein A is unaffected by ssDNA binding
Replication protein A (RPA) is a heterotrimeric, multi-functional protein that binds single-stranded DNA (ssDNA) and is essential for eukaryotic DNA metabolism. Using heteronuclear NMR methods we have investigated the domain interactions and ssDNA binding of a fragment from the 70 kDa subunit of human RPA (hRPA70). This fragment contains an N-terminal d
Oxford University Press.