Heat Pulse
Mostrando 13-24 de 101 artigos, teses e dissertações.
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13. Ubiquitous overexpression of a transgene encoding the extracellular portion of the Drosophila Roughest-Irregular Chiasm C protein induces early embryonic lethality
The cell adhesion molecule Rst-irreC is a transmembrane glycoprotein of the immunoglobulin superfamily involved in several important developmental processes in Drosophila, including axonal pathfinding in the optic lobe and programmed cell death and pigment cell differentiation in the pupal retina. As an initial step towards the "in vivo'' functional analysis
Anais da Academia Brasileira de Ciências. Publicado em: 2000-09
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14. Study of the transport and storage properties of electric charges in Teflon FEP films. / Estudo das propriedades de transporte e armazenamento de cargas elétricas em filmes de Teflon FEP.
The Heat Pulse and TSC (Thermo-Stimulated-Current) in open circuit techniques were used to study the transport and storage of injected charge in Teflon FEP films. Using these techniques it was possible to follow the evolution of the injected charge inside the samples. Virgin samples (as received), previously annealed samples and previously ionic bombarded sa
Publicado em: 1986
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15. Montagem da técnica de pulso térmico e sua aplicação ao estudo da evolução de cargas termicamente ativadas da superfície. / The heat pulse technique and its application to the study of the evolution of thermally stimulated charges from the surface.
Theoretical experimental aspects of the Heat Pulse Technique (HPT) are presented. The applications were the measurements of surface potentials, charge centroid, and total charge of the film samples. No tentative was made to determinate the spatial distribution of the observed charges. The HPT was used as complement of TSC open circuits measurements in the st
Publicado em: 1983
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16. FLP Recombinase-Mediated Induction of Cu/Zn-Superoxide Dismutase Transgene Expression Can Extend the Life Span of Adult Drosophila melanogaster Flies
Yeast FLP recombinase was used in a binary transgenic system (“FLP-OUT”) to allow induced overexpression of catalase and/or Cu/Zn-superoxide dismutase (Cu/ZnSOD) in adult Drosophila melanogaster. Expression of FLP recombinase was driven by the heat-inducible hsp70 promoter. Once expressed, FLP catalyzed the rearrangement and activation of a target constr
American Society for Microbiology.
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17. Operator-promoter functions in the threonine operon of Escherichia coli.
The prophage curing properties of secondary-site lysogens of coliphage lambda have been studied. The site of integration in the original lysogen (L79) is within the ooerator-promoter region of the thr operon. As a result, expression of the thr enzymes is reduced, and the strain is a leaky threonine auxotroph. Heat pulse curing of strain L79 and a thr+ lysoge
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18. Two closely linked transcription units within the 63B heat shock puff locus of D. melanogaster display strikingly different regulation.
We report the isolation and characterization of a cloned DNA of D. melanogaster, Dm4L, that is derived from the major heat shock puff site at 63B. This segment contains two closely linked genes that are each present once per Drosophila haploid genome. One of these, the hsp 83 gene, encodes an abundant heat shock mRNA that, unlike other major heat shock mRNAs
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19. Influence of Polyethylene Glycol on the Size of Schizosaccharomyces pombe Electropores
The role of polyethylene glycol (PEG) in the transformation of Schizosaccharomyces pombe by electroporation is investigated by fluorescein isothiocyanate-dextran uptake and transformation studies. It is shown that when S. pombe cells are electroporated in the presence of PEG, the permeability state created is sustained until removal of PEG. In addition, the
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20. Dynamic interplay between antagonistic pathways controlling the σ32 level in Escherichia coli
The heat-shock response in Escherichia coli depends primarily on the transient increase in the cellular level of heat-shock sigma factor σ32 encoded by the rpoH gene, which results from both enhanced synthesis and transient stabilization of normally unstable σ32. Heat-induced synthesis of σ32 was previously shown to occur at the translation level by
The National Academy of Sciences.
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21. Heat Shock Protein-Mediated Resistance to High Hydrostatic Pressure in Escherichia coli
A random library of Escherichia coli MG1655 genomic fragments fused to a promoterless green fluorescent protein (GFP) gene was constructed and screened by differential fluorescence induction for promoters that are induced after exposure to a sublethal high hydrostatic pressure stress. This screening yielded three promoters of genes belonging to the heat shoc
American Society for Microbiology.
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22. Effect of Heat Shock on the Metabolism of Glutathione in Maize Roots 1
High performance liquid chromatography analyses revealed that glutathione (GSH) and cysteine are two of the major low molecular weight thiol compounds in maize root extracts. Treatment of maize roots to heat shock temperatures of 40°C resulted in a decrease of cysteine levels and an increase of GSH levels. Pulse labeling of maize roots with [35S]cysteine sh
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23. Characterization of heat shock in Bacillus subtilis.
We characterized the general properties of the heat shock response in Bacillus subtilis W168, B. subtilis JH642, and an spo0A mutant by using pulse-labeling of bacterial proteins and one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The transfer of cells from 37 to 50 degrees C repressed synthesis of most cellular proteins and led to
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24. Spore coat protein and enterotoxin synthesis in Clostridium perfringens.
Polyacrylamide gel profiles of Clostridium perfringens spore coat protein revealed four and occasionally five components. Pulse-chase experiments indicated that synthesis of coat protein polypeptide and enterotoxin was an early sporulation event. However, maximum synthesis occurred coincident with the onset of heat resistance.