Hansenula Wingei
Mostrando 13-24 de 24 artigos, teses e dissertações.
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13. Sporulation in Hansenula wingei Is Induced by Nitrogen Starvation in Maltose-Containing Media
The sexually agglutinative yeast Hansenula wingei lives in association with bark beetles that inhabit coniferous trees. This yeast was induced to sporulate by malt extract, which contains a high percentage of maltose (50%) and a low percentage of nitrogen (0.5%). A solution of 1.5% maltose without any growth factors also induced ascosporogenesis in H. wingei
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14. Microtiter plate assay for sexual agglutination in the yeast Hansenula wingei.
A fast method is described for determining the mating type of yeast spore clones independent of genetic complementation between auxotrophic markers.
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15. Cell-cell recognition in yeast: Purification of Hansenula wingei 21-cell sexual agglutination factor and comparison of the factors from three genera
Trypsin digestion of Hansenula wingei 21-cells releases a protein (21-factor-T) that inhibits the agglutination of 21-cells by purified 5-agglutinin obtained from 5-cells by subtilisin digestion [Crandall, M. A. & Brock, T. D. (1968) Bacteriol. Rev. 32, 139-163]. We have purified this inhibitor 415-fold by ion-exchange chromatography, affinity adsorption to
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16. Electron Microscopy of Cell Fusion in Conjugating Hansenula wingei
Conti, S. F. (Dartmouth Medical School, Hanover, N.H.), and T. D. Brock. Electron microscopy of cell fusion in conjugating Hansenula wingei. J. Bacteriol. 90:524–533. 1965.—The heterothallic yeast Hansenula wingei is a favorable organism for the study of the process of cell fusion, since strong agglutination of cells of the two mating types ensures a hig
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17. Rare-Cell Fusion Events between Diploid and Haploid Strains of the Sexually Agglutinative Yeast HANSENULA WINGEI
Diploids of the yeast Hansenula wingei are nonagglutinative and do not form zygotes in mixed cultures with either sexually agglutinative haploid mating type. However, a low frequency of diploid x haploid cell fusions (about 10-3) is detectable by prototrophic selection. This frequency of rare diploid x haploid matings is not increased after the diploid cultu
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18. A Microbiological Assay for Estimating Concentrations of Camptothecin Lactone in Mouse Tissues
The marked sensitivity of the yeast Hansenula wingei NRRL Y-2340 strain 5 to a new antineoplastic alkaloid served as the basis for a logarithmic-ratio microbiological assay which has been developed for the estimation of concentrations of camptothecin lactone (or cytotoxic equivalents) in tissues of mice. The utility of this assay has been demonstrated by pre
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19. Molecular Complementarity of Yeast Glycoprotein Mating Factors
Cell fusion between opposite mating types 5 and 21 of the yeast Hansenula wingei is initiated by a strong sexual agglutination reaction. The mating factors responsible for the specificity of cellular recognition are complementary glycoproteins which form a physical complex in vitro. The complex is assayed by recovery of agglutination activity of the multival
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20. A conserved sequence element is present around the transcription initiation site for RNA polymerase A in Saccharomycetoideae.
To identify DNA elements involved in the initiation of rRNA transcription in yeast we located the start site of the rRNA operon of Kluyveromyces lactis and Hansenula wingei, both members of the Saccharomycetoideae, by S1 nuclease analysis and determined the surrounding nucleotide sequences. Comparison of these sequences with those of Saccharomyces carlsberge
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21. Biochemical and Cellular Changes Occurring During Conjugation in Hansenula wingei
Brock, Thomas D. (Indiana University, Bloomington). Biochemical and cellular changes occurring during conjugation in Hansenula wingei. J. Bacteriol. 90:1019–1025. 1954.—A technique has been devised for deagglutinating mixed populations of conjugating cells so as to be able to visualize microscopically early stages of the conjugation process. A cell can f
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22. Comparison of RapID Yeast Plus System with API 20C System for Identification of Common, New, and Emerging Yeast Pathogens
The ability to identify yeast isolates by the new enzymatic RapID Yeast Plus System was compared to the ability to identify yeast isolates by the API 20C system. A total of 447 yeast isolates representing Blastoschizomyces capitatus, 17 Candida spp., 5 Cryptococcus spp., Geotrichum spp., 2 Hanseniaspora spp., Hansenula anomala, Hansenula wingei, 3 Rhodotorul
American Society for Microbiology.
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23. Separate structural elements within internal transcribed spacer 1 of Saccharomyces cerevisiae precursor ribosomal RNA direct the formation of 17S and 26S rRNA.
Structural features of Internal Transcribed Spacer 1 (ITS1) that direct its removal from Saccharomyces cerevisiae pre-rRNA during processing were identified by an initial phylogenetic approach followed by in vivo mutational analysis of specific structural elements. We found that S. cerevisiae ITS1 can functionally be replaced by the corresponding regions fro
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24. Mating Pheromones of Saccharomyces kluyveri: Pheromone Interactions Between Saccharomyces kluyveri and Saccharomyces cerevisiae
Saccharomyces kluyveri is a heterothallic yeast with two allelic mating types denoted as a-k and α-k by analogy with Saccharomyces cerevisiae and from the work described here. S. kluyveri produces mating pheromones analogous to those of S. cerevisiae, but which appear to have different specificity. S. kluyveri thus differs from S. cerevisiae, Hansenula wing