Gtpase Activity
Mostrando 1-12 de 678 artigos, teses e dissertações.
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1. Estudos funcionais e estruturais das septinas humanas 6, 8 e 10 / Functional and structural studies of human septin 6, 8 and 10
A cell passes through some steps to enable for cell division and all these processes are called Cell Cycle. The spatial and temporal regulation of this process is crucial to maintaining cellular and genetic material. Failure in this process can lead to cell death or genetic changes causing division and unregulated growth of tumors. Among several proteins inv
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 05/03/2010
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2. Estudos estruturais e bioquímicos das septinas 7 e 9 humanas / Structural and biochemical studies of human septins 7 e 9
As proteínas pertencentes à família das septinas foram originalmente descobertas em 1971 em decorrencia de estudos genéticos em células mutantes. Essas proteínas encontradas em fungos e animais, mas não em plantas apresentam como principais características a presença de um domínio conservado de ligação aos nucleotídeos de guanina (GTP) e a forma
Publicado em: 2010
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3. Estudos estruturais da septina humana SEPT11 / STRUCTURAL STUDIES OF THE HUMAN SEPTIN SEPT11
Septinas são proteínas de ligação ao nucleotídeo de guanina (GTP). Foram inicialmente identificadas em fungos e atuam na fase final da divisão celular. Posteriormente, também verificaram que esta família de proteínas está presente em outros eucariotos com exceção de plantas. Septinas são purificadas de fungos Saccharomyces cerevisiae, Drosophila
Publicado em: 2008
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4. Structural analyses of rab family GTPases and mechanism of Mafb regulation by the protein TIPRL / Analises estruturais de GTPases da familia RAB e mecanismo de regulção de MAFB pela proteina TIPRL
GTPases of the Rab family are responsible for the intracellular transport of vesicles. Each family member acts on a specific transport pathway and their function is regulated by GTP binding and hydrolysis, cycling between inactive (GDP-bound) and active (GTP-bound) forms. In this work, we describe the crystal structure of inactive and active forms of the GTP
Publicado em: 2007
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5. Identificação de atividades ATPásica e Amilásica em larvas de Pachymerus nucleorum (Fabricius, 1792) (Coleoptera: Chrysomelidae: Bruchinae)
ABSTRACT - CHAPTER I - Pachymerus nucleorum (Fabricius, 1792) is a beetle specie of the Bruchinae sub-family, family Chysomelidae, that principal host of this insect is a palm babassu (Orbinya spp). The objective of this work was identify and to characterize the ATPase activity in P. Nucleorum larvae. The P. nucleorum larvae guts were discartated and the gre
Publicado em: 2006
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6. Expression of active BCR related (ABR) gene in chronic myeloid leukemia (CML) real-time RT-PCR (qPCR) / Estudo da expressão do Gene ABR em leucemia mieloide cronica (LMC) utilizando real-time RT-PCR (qPCR)
Chronic myeloid leukemia (CML) is a clonal proliferative disorder of the hematopoietic stem cell cytogenetically characterized by the Philadelphia (Ph) chromosome, a result of chromosomal translocation t(9;22)(q34;q11). At molecular level, the Ph chromosome results in a fusion gene, the chimerical BCR-ABL which has constitutive tyrosine kinase activity and i
Publicado em: 2006
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7. Capillary electrophoresis-based biomolecular recognition studies / Estudos de reconhecimento biomolecular por eletroforese capilar
This Thesis concerns on the development of capillary electrophoresis-based bioanalytical methods for two distinct applications: DNA-protein binding analysis and monitoring of enzyme-catalyzed GTP hydrolysis. In the first chapter, the feasibility of on-chip electrophoretic mobility-shift assays (EMSA) is investigated. Purified transcription factors c-Jun(AP1)
Publicado em: 2005
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8. Plasmodium yoelii: identification of a gene encoding a putative ADP-ribosylation factor-1 GTPase-activating Protein, PyAG1
The PyAG1 gene, identified by the screening of a Plasmodium yoelii genomic DNA library with a rhoptry-specific Mab, encodes a protein with a zinc finger structure immediately followed by the consensus sequence of the Arf GAP catalytic site. The serum of mice immunized with the recombinant protein recognized specifically the rhoptries of the late infected ery
Memórias do Instituto Oswaldo Cruz. Publicado em: 2000-06
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9. Intrinsic GTPase activity distinguishes normal and oncogenic ras p21 molecules.
The 21-kilodalton protein (p21) encoded by normal cellular Harvey-ras has been expressed in Escherichia coli as a fusion protein by using the pUC8 vector and has been purified to greater than 95% homogeneity by ion-exchange chromatography and gel filtration. The purified protein molecules possess intrinsic GTPase activity on the basis of the following criter
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10. Ha-ras proteins exhibit GTPase activity: point mutations that activate Ha-ras gene products result in decreased GTPase activity.
Several ras genes have been expressed at high levels in Escherichia coli and the resultant ras proteins were shown to be functional with respect to their well-known specific, high-affinity, GDP/GTP binding. We were able to detect a weak GTPase activity associated with the purified proteins. The normal cellular ras protein (p21N) exhibits approximately equal
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11. Light-activated guanosinetriphosphatase in Musca eye membranes resembles the prolonged depolarizing afterpotential in photoreceptor cells.
Measurement of light-dependent GTPase (EC 3.1.5.1) activity in a paradigm guided by electrophysiological experiments was used to examine the involvement of a guanine nucleotide binding protein in fly phototransduction. Cell-free membrane preparations of Musca eyes responded to blue light by a 10- to 20-fold increase in GTP-hydrolyzing activity. This light-de
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12. Characterization of GTPase Activity of TrmE, a Member of a Novel GTPase Superfamily, from Thermotoga maritima
A gene encoding a putative GTP-binding protein, a TrmE homologue that is highly conserved in both prokaryotes and eukaryotes, was cloned from Thermotoga maritima, a hyperthermophilic bacterium. T. maritima TrmE was overexpressed in Escherichia coli and purified. TrmE has a GTPase activity but no ATPase activity. The GTPase activity can be competed with GTP,
American Society for Microbiology.