Granular Synthesis
Mostrando 13-24 de 49 artigos, teses e dissertações.
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13. Angiotensin II immunoreactivity coexists with renin in the juxtaglomerular granular cells of the kidney.
The multiple physiologic functions of angiotensin II(AII) are generally supposed to be mediated by the peptide generated in the blood circulation. In addition to this extracellular mechanism of AII formation, we have obtained immunohistochemical evidence for the intracellular synthesis of AII in the kidney. Rats were perfused with fixative, and paraffin sect
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14. Differentiation-specific expression from the bovine papillomavirus type 1 P2443 and late promoters.
The papillomavirus life cycle is tightly linked with keratinocyte differentiation in squamous epithelia. Vegetative viral DNA replication begins in the spinous layer, while synthesis of capsid proteins and virus maturation is restricted to the most differentiated or granular layer of the epithelium. In this study, in situ hybridization of bovine fibropapillo
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15. The cytolytic protein of human lymphocytes related to the ninth component (C9) of human complement: isolation from anti-CD3-activated peripheral blood mononuclear cells.
A 70-kDa channel-forming protein has recently been isolated from human large granular lymphocytes maintained in interleukin-2-dependent culture. The protein was shown to be immunochemically related to the ninth component of complement (C9) and was therefore designated C9-related protein (C9RP). Using the procedure that was developed for the isolation of C9RP
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16. The effect of actinomycin D on the nucleolus and on pigment synthesis in pigment cells of Xenopus laevis: an ultrastructural study.
The effect of AMD on the nucleolus and on melanogenesis in differentiating pigment cells of Xenopus laevis was investigated in cultured neural crest cells. Cultures were treated with either 2 or 10 microgram/ml AMD for 41/2 hours. Following treatment the antibiotic was removed. Observations of the cells were made with both scanning and transmission electron
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17. Sites of Synthesis and Processing of Ribosomal RNA Precursors within the Nucleolus of Urechis caupo Eggs*
Nucleoli from unfertilized Urechis eggs, labeled with tritiated RNA precursors, have been isolated for simultaneous autoradiographic localization and biochemical analysis of labeled RNA. The production of the ribosomal RNA precursor (38S) and its first cleavage occur at the fibrillar core region of the nucleolus. The products, predominantly 30S RNA, are then
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18. Effect of the Inhibition of Protein Synthesis on the Escherichia coli Cell Envelope
The consequences for cell envelope integrity of Escherichia coli K-12 of the inhibition of protein synthesis by a variety of means have been examined. Protein synthesis was blocked by the antibiotics chloramphenicol and streptomycin, by amino acid starvation of an amino acid auxotroph, and by inactivation of temperature-sensitive aminoacyl transfer ribonucle
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19. Storage, Photosynthesis, and Growth: The Conditional Nature of Mutations Affecting Starch Synthesis and Structure in Chlamydomonas.
Growth-arrested Chlamydomonas cells accumulate a storage polysaccharide that bears strong structural and functional resemblance to higher plant storage starch. It is synthesized by similar enzymes and responds in an identical fashion to the presence of mutations affecting these activities. We found that log-phase photosynthetically active algae accumulate gr
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20. Localization of glucocorticoid receptor mRNA in the male rat brain by in situ hybridization.
The localization and distribution of mRNA encoding the glucocorticoid receptor (GR) was investigated in tissue sections of the adult male rat brain by in situ hybridization and RNA blot analysis. GR mRNA levels were measured by quantitative autoradiography with 35S- and 32P-labeled RNA probes, respectively. Strong labeling was observed within the pyramidal n
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21. Formation of cytoplasmic heat shock granules in tomato cell cultures and leaves.
Biochemical and electron microscopic analyses of heat-shocked suspension cultures of Peruvian tomato (Lycopersicon peruvianum) revealed that a considerable part of the dominant small heat shock proteins (hsps) with an Mr of approximately 17,000 are structural proteins of newly forming granular aggregates in the cytoplasm (heat shock granules), whose formatio
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22. Identification of aldosterone-induced proteins in the toad's urinary bladder.
Aldosterone (the 8,11-hemiacetal 0f 11beta,21-dihydroxy-3,20-dioxo-4-pregnen-18-al) markedly stimulates sodium transport in a number of epithelial tissues. We have attempted to determine whether aldosterone induces the synthesis of specific protein(s) in the course of its action upon the toad urinary bladder. Paired hemibladders were incubated in media conta
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23. New data concerning the functional organization of the mammalian cell nucleolus: detection of RNA and rRNA by in situ molecular immunocytochemistry.
We have investigated the fine spatial distribution of RNA and rRNA within the Ehrlich tumor cell nucleolus by in situ hybridization with a biotin-labeled probe and by two new strategies, the polyadenylate nucleotidyl transferase-immunogold technique and immuno-labeling with anti-RNA antibodies. Besides the presence, as expected, of RNA and rRNA in the granul
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24. Genetic and Biochemical Evidence for the Involvement of α-1,4 Glucanotransferases in Amylopectin Synthesis1
We describe a novel mutation in the Chlamydomonas reinhardtii STA11 gene, which results in significantly reduced granular starch deposition and major modifications in amylopectin structure and granule shape. This defect simultaneously leads to the accumulation of linear malto-oligosaccharides. The sta11-1 mutation causes the absence of an α-1,4 glucanotrans
American Society of Plant Physiologists.