Goldfish
Mostrando 13-24 de 106 artigos, teses e dissertações.
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13. Cloning and localization of two multigene receptor families in goldfish olfactory epithelium
Goldfish reproduction is coordinated by pheromones that are released by ovulating females and detected by males. Two highly potent pheromones, a dihydroxyprogesterone and a prostaglandin, previously have been identified, and their effects on goldfish behavior have been studied in depth. We have cloned goldfish olfactory epithelium cDNAs belonging to two mult
The National Academy of Sciences.
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14. Multiple loci for synapse protein SNAP-25 in the tetraploid goldfish.
The common goldfish Carassius auratus is tetraploid and has 100 chromosomes. We describe here goldfish cDNA clones for SNAP-25, a 200-amino-acid synaptosome-associated protein that has remained highly conserved during evolution. SNAP-25 occurs as a single-copy gene in mouse, chicken, and Drosophila melanogaster. Sequences of six distinct goldfish cDNA clones
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15. A plasminogen activator is induced during goldfish optic nerve regeneration.
The use of purified piscine plasminogen in a chromogenic solution assay enabled us to detect plasminogen activator (PA) activity in crude homogenates of goldfish optic nerve following nerve injury. In contrast, no activity was detected in the homogenates of uninjured nerve. Under conditions allowing regeneration of the optic axons (optic nerve crush), PA act
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16. Effect of potassium depolarization on sodium-dependent calcium efflux from goldfish heart ventricles and guinea-pig atria
1. 45Ca fluxes were studied in normal and potassium-depolarized goldfish ventricles as a function of the external Na concentration. Some of the experiments were also performed on guinea-pig auricles.
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17. Toxic Effect of Staphylococcal Lysins for Goldfish1
Goldfish died within 24 hr after intraperitoneal injections of 0.2 ml of Seitz filtrates of hemolytic Staphylococcus aureus cultures grown on Dolman and Wilson medium under increased CO2 pressure for 72 to 96 hr. Two lethal toxins differing in heat sensitivity, antigenicity, and degree of toxicity were demonstrated. Studies of the relationship between the le
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18. Goldfish, Carassius auratus, a Novel Animal Model for the Study of Mycobacterium marinum Pathogenesis
We have developed an animal model for studying mycobacterial pathogenesis using Mycobacterium marinum and the goldfish, Carassius auratus. Goldfish are injected intraperitoneally with doses between 102 and 109 CFU of M. marinum organisms. Depending on the dose of M. marinum organisms administered, an acute or chronic disease is produced. The acute disease is
American Society for Microbiology.
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19. Isolation of cDNA clones encoding RICH: a protein induced during goldfish optic nerve regeneration with homology to mammalian 2',3'-cyclic-nucleotide 3'-phosphodiesterases.
Using data derived from peptide sequencing of p68/70, a protein doublet induced during optic nerve regeneration in goldfish, we have isolated cDNAs that encode RICH (regeneration-induced CNPase homolog) from a goldfish regenerating retina cDNA library. The predicted RICH protein comprises 411 amino acids, possesses a pI of 4.48, and shows significant homolog
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20. Genetic and Developmental Analysis of Some New Color Mutants in the Goldfish, CARASSIUS AURATUS
The genotypes of three color mutants in goldfish: a depigmentation character of larval melanophores, albinism and a recessive-transparent character, were analyzed by crossing experiments. The depigmentation character in the common goldfish is controlled by two dominant multiple genes, Dp 1 and Dp2, and only fish with double recessive alleles dp1dp1 dp2dp2 ca
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21. Dibutyryl Adenosine Cyclic 3′:5′-Monophosphate Effects on Goldfish Behavior and Brain RNA Metabolism
Intraventricular administration of dibutyryl adenosine cyclic 3′:5′-monophosphate into goldfish brains produced hyperactive animals. A study of the effects of the drug (25-50 mg/kg) on the incorporation of [5-3H] orotic acid, as a precursor of labeled uridine and cytidine, into newly synthesized RNA showed the formation of an RNA with a uridine to cytidi
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22. Enhancement of Goldfish Virus Type 2 Replication by 1- Naphthol, the Major Hydrolysis Product of the Pesticide Carbaryl
The major hydrolysis product of the pesticide carbaryl, 1-naphthol, enhanced the replication of goldfish virus type 2 in piscine cell culture at concentrations below any detectable cytotoxicity. Enhancement indices of 2.3, 3.7, and 7.1 were observed at 1, 5, and 10 ppm (μl/liter), respectively. Replication at 0.5 ppm was equivalent to that of controls.
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23. Brain protein metabolism and the acquisition of new patterns of behavior.
Three cytoplasmic proteins (designated alpha, beta and gamma) in the goldfish brain consistently incorporated more labeled valine in animals that had acquired a new pattern of swimming behavior than in untrained animals. The changes were identified by double labeling techniques ([3H]valine injected into trained experimental animals and [14C]valine into untra
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24. DL-2-amino-4-phosphonobutyric acid does not eliminate "ON" responses in the visual system of goldfish.
DL-2-Amino-4-phosphonobutyric acid (APB) suppresses activity in retinal ON pathways. It is generally assumed that loss of the ON pathway would result in loss of ON responses in the visual system. We tested this assumption by recording activity from the optic nerves of intact goldfish (Carassius auratus) before and after intraocular injection of APB. Whole-ne