Glycoside Hydrolases
Mostrando 1-12 de 27 artigos, teses e dissertações.
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1. The genome sequence of Dyella jiangningensis FCAV SCS01 from a lignocellulose-decomposing microbial consortium metagenome reveals potential for biotechnological applications
Abstract Cellulose and its associated polymers are structural components of the plant cell wall, constituting one of the major sources of carbon and energy in nature. The carbon cycle is dependent on cellulose- and lignin-decomposing microbial communities and their enzymatic systems acting as consortia. These microbial consortia are under constant exploratio
Genet. Mol. Biol.. Publicado em: 14/05/2018
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2. Draft genome sequence of Streptomyces sp. strain F1, a potential source for glycoside hydrolases isolated from Brazilian soil
ABSTRACT Here, we show the draft genome sequence of Streptomyces sp. F1, a strain isolated from soil with great potential for secretion of hydrolytic enzymes used to deconstruct cellulosic biomass. The draft genome assembly of Streptomyces sp. strain F1 has 69 contigs with a total genome size of 8,142,296 bp and G + C 72.65%. Preliminary genome analysis iden
Braz. J. Microbiol.. Publicado em: 2017-12
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3. Hemolytic activity of venom from crown-of-thorns starfish Acanthaster planci spines
Background : The crown-of-thorns starfish Acanthaster planci is a venomous species from Taiwan whose venom provokes strong hemolytic activity. To understand the hemolytic properties of A. planci venom, samples were collected from A. planci spines in the Penghu Islands, dialyzed with distilled water, and lyophilized into A. planci spine venom (ASV) powder. R
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 25/09/2013
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4. Molecular bases of the specificity substrate of a β-glicodase / Bases moleculares da especificidade pelo substrato em β-glicosidases
The β-glycosidases of family 1 of the glycoside hydrolases (GH 1) are one of the most important groups of enzymes. These enzymes are involved in a high diversity of physiological functions. The main objective of this study was the analysis of the molecular bases of the specificity for substrate of a β-glycosidase from the larvae of Spodoptera frugi
Publicado em: 2009
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5. Structural and functional studies of two glycosyl hydrolases : the putaitve cellulase XF0810 of Xylella fastidiosa and the digestive lysozyme 1 of Musca domestica / Estudos estruturais e funcionais de duas glicosideo hidrolases : a celulase putativa XF0810 de Xylella fastidiosa e a lisozima digestiva 1 de Musca domestica
Glicosídeo hidrolases (EC 3.2.1.-) são enzimas que hidrolisam ligações glicosídicas. Neste trabalho realizamos estudos funcionais e estruturais de duas glicosídeo hidrolases: a celulase XF0810 de Xylella fastidiosa e a lisozima digestiva 1 de Musca domestica (MdL1). A celulase XF0810 está anotada no genoma da X. fastidiosa como membro da família 5 da
Publicado em: 2007
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6. Purificação, caracterização, clonagem e seqüenciamento de β-glicosidades de Tenebrio molitor (Coleoptera) / Purification, characterization, cloning and sequencing of β-glycosidases from Tenebrio molitor (Coleoptera)
In the midgut lumen of Tenebrio molitor larvae there are 4 β-glycosidases (named 1, 2, 3a and 3b), not present in the animal food. They were purified with electrophoresis, ion exchange and hydrophobic chromatographies. β-glycosidase 1 (relative molecular weight - Mr 59,000) is unstable at 30°C but is stabilized by substrates. The enzyme hydrolyses
Publicado em: 2001
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7. α-Galactosidase Aga27A, an Enzymatic Component of the Clostridium josui Cellulosome
The Clostridium josui aga27A gene encodes the cellulosomal α-galactosidase Aga27A, which comprises a catalytic domain of family 27 of glycoside hydrolases and a dockerin domain responsible for cellulosome assembly. The catalytic domain is highly homologous to those of various α-galactosidases of family 27 of glycoside hydrolases from eukaryotic organisms,
American Society for Microbiology.
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8. The Family-3 Glycoside Hydrolases: from Housekeeping Functions to Host-Microbe Interactions
American Society for Microbiology.
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9. Differential Recognition and Hydrolysis of Host Carbohydrate Antigens by Streptococcus pneumoniae Family 98 Glycoside Hydrolases*
The presence of a fucose utilization operon in the Streptococcus pneumoniae genome and its established importance in virulence indicates a reliance of this bacterium on the harvesting of host fucose-containing glycans. The identities of these glycans, however, and how they are harvested is presently unknown. The biochemical and high resolution x-ray crystall
American Society for Biochemistry and Molecular Biology.
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10. Purification of glycoside hydrolases from Bacteroides fragilis.
Six glycoside hydrolases in the culture medium of Bacteroides fragilis--alpha-glucosidase, beta-glucosidase, alpha-galactosidase, beta-galactosidase, beta-N-acetylglucosaminidase, and alpha-L-fucosidase-were systematically purified by ammonium sulfate precipitation, gel filtration chromatography, and density gradient isoelectric focusing. The isoelectric foc
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11. Ruminococcus albus 8 Mutants Defective in Cellulose Degradation Are Deficient in Two Processive Endocellulases, Cel48A and Cel9B, Both of Which Possess a Novel Modular Architecture
The cellulolytic bacterium Ruminococcus albus 8 adheres tightly to cellulose, but the molecular biology underpinning this process is not well characterized. Subtractive enrichment procedures were used to isolate mutants of R. albus 8 that are defective in adhesion to cellulose. Adhesion of the mutant strains was reduced 50% compared to that observed with the
American Society for Microbiology.
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12. Molecular Characterization and Expression in Escherichia coli of Three β-1,3-Glucanase Genes from Lysobacter enzymogenes Strain N4-7
Lysobacter enzymogenes strain N4-7 produces multiple biochemically distinct extracellular β-1,3-glucanase activities. The gluA, gluB, and gluC genes, encoding enzymes with β-1,3-glucanase activity, were identified by a reverse-genetics approach following internal amino acid sequence determination of β-1,3-glucanase-active proteins partially purified from
American Society for Microbiology.