Giant Vesicles
Mostrando 1-12 de 57 artigos, teses e dissertações.
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1. Effects of artepillin C on model membranes displaying liquid immiscibility
It has been hypothesized that the therapeutic effects of artepillin C, a natural compound derived from Brazilian green propolis, are likely related to its partition in the lipid bilayer component of biological membranes. To test this hypothesis, we investigated the effects of the major compound of green propolis, artepillin C, on model membranes (small and g
Braz J Med Biol Res. Publicado em: 25/03/2019
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2. Study on the Application of Electric Field to Giant Vesicles Comprised of 1,2-Dilauroyl-Sn-Glycero-3-Phosphatidylcholine Using Optical Fluorescence Microscopy
The influence of alternating electric field (AC) in the structure and dynamics of giant unilamellar vesicles (GUVs) comprised of 1,2-dilauroyl-sn-glycero-3-phosphatidylcholine (DLPC) is reported. The investigations were conducted by using optical fluorescence microscopy as the method of analysis. The lipid membrane of the DLPC GUVs at the fluid phase can be
Mat. Res.. Publicado em: 18/05/2017
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3. Efeito da carga dos lipídios na interação do BP100 em modelos de membrana / Charge effect of lipids in interaction of BP100 and model membranes
Antimicrobial peptides (AMPs) have been a promising alternative therapy to antibiotics. These molecules, consisting of less than 80 amino acids, with different structures and amino acids composition, have amphipathic character which gives them the ability to act on lipid bilayers. They act, by different mechanisms, over bacteria to mammal cells. The AMPs are
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 17/02/2012
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4. Fotossensibilização de vesículas lipídicas gigantes / Giant Lipid Vesicles Photosensibilization
A Terapia Fotodinâmica é um tratamento promissor no cura de várias doenças oftalmológicas e dermatológicas, assim como tumores. Este tratamento utiliza a combinação de luz e um composto fotossensível na presença de oxigênio. Neste trabalho objetivamos entender mecanismos de fotossensibilização em membranas. Para isso, estudamos os efeitos de irr
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 20/12/2011
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5. Interaction of hidrophilic porphyrins and extracellular hemoglobin with biomimetic models of biological membranes / Interação de porfirinas hidrofílicas e de hemoglobina extracelular com modelos biomiméticos de membrana biológica
In the first part of this work interactions of the cationic meso tetrakis (4-N-methilpyridil) porphyrin (TMPyP) and meso-tetrakis (4-sulfonatophenyl) porphyrin (TPPS4) in the free base forms with membrane model systems (ionic micelles, mixed micelles and phospholipids vesicles) in aqueous solutions, have been investigated by optical absorption, resonance lig
Publicado em: 2008
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6. Propriedades de vesículas unilamelares gigantes / Properties of Giant Unillamelar Vesicles
The stability of giant unilamellar vesicles (GUVs) has been monitored by phase contrast and fluorescence microscopy, using sugar gradients, sodium 1,3,6,8 pirene tetrasulfonate (PTS) as fluorescent probe, 1,1-dimethyl-4,4-bipiridinium chloride (MV) as fluorescence quencher and 2-(12-(7-nitrobenz-2-oxa-1,3-diazol-4-il) amino) dodecanoyl-1-hexadecanoyl-sn-glic
Publicado em: 2006
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7. Proteins with mechanical functionality: a physico-chemical study on the viscoelasticity of gliadin, a storage protein from wheat gluten / Proteínas com funcionalidade mecânica: um estudo físico-químico sobre a viscoelasticidade da gliadina, uma proteína de reserva do glúten do trigo
The thesis consisted in a rheological study of the dynamics of gels and dispersions of gliadin from wheat gluten in pure dimethylsulfoxide, dimethylformamide or formamide and in their mixtures with water. The effect of temperature and that of the concentration of the component species (protein and solvent constituents) on flow behavior and deformation were e
Publicado em: 2004
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8. ATP-dependent directional movement of rat synaptic vesicles injected into the presynaptic terminal of squid giant synapse.
The question as to whether synaptic vesicles prepared from vertebrate brain can be transported to the active zones of the squid giant synapse was studied by using a combined optical and electrophysiological approach. In order to visualize the behavior of the vertebrate synaptic vesicles in situ, synaptic vesicles isolated from rat brain were labeled with a f
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9. Calpain activity promotes the sealing of severed giant axons
A barrier (seal) must form at the cut ends of a severed axon if a neuron is to survive and eventually regenerate. Following severance of crayfish medial giant axons in physiological saline, vesicles accumulate at the cut end and form a barrier (seal) to ion and dye diffusion. In contrast, squid giant axons do not seal, even though injury-induced vesicles for
The National Academy of Sciences of the USA.
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10. Asymmetric Oxidation of Giant Vesicles Triggers Curvature-Associated Shape Transition and Permeabilization
Oxidation of unsaturated lipids is a fundamental process involved in cell bioenergetics as well as in cell death. Using giant unilamellar vesicles and a chlorin photosensitizer, we asymmetrically oxidized the outer or inner monolayers of lipid membranes. We observed different shape transitions such as oblate to prolate and budding, which are typical of membr
The Biophysical Society.
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11. Shape change and physical properties of giant phospholipid vesicles prepared in the presence of an AC electric field.
Giant unilamellar vesicles with diameters ranging from 10 to 60 microns were obtained by the swelling of phospholipid bilayers in water in the presence of an AC electric field. This technique leads to a homogeneous population of perfectly spherical and unilamellar vesicles, as revealed by phase-contrast optical microscopy and freeze-fracture electron microsc
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12. Rapid preparation of giant unilamellar vesicles.
We report here a rapid evaporation method that produces in high yield giant unilamellar vesicles up to 50 microns in diameter. The vesicles are obtained after only 2 min and can be prepared from different phospholipids, including L-alpha-phosphatidylcholine (lecithin), dipalmitoleoyl L-alpha-phosphatidylcholine, and beta-arachidonoyl gamma-palmitoyl L-alpha-