Genes Tat
Mostrando 1-12 de 189 artigos, teses e dissertações.
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1. Reprogramação de células mesenquimais de tecido adiposo em células-tronco pluripotentes por meio de proteína de fusão TAT / Nuclear reprogramming of adipose-tissue mesenchymal stem cells into pluripotent stem cells using TAT fusion protein
Os vírus são eficazes na transferência de genes em células devido aos seus mecanismos especializados. No entanto, vírus como veículos de entrega de genes podem acarretar em problemas, particularmente quando proposto para reprogramar células somáticas em células-tronco pluripotentes induzidas (iPS) visando utilização terapêutica. No presente estud
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 23/02/2012
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2. Estudo do direcionamento das proteases FtsH plastidiais às membranas dos tilacóides / Study of plastidial FtsH proteases targeting to thylakoid membranes
O complexo FtsH em Arabidopsis, presente nos tilacóides, é formado pelas subunidades FtsH1/FtsH5 (tipo A) e FtsH2/FtsH8 (tipo B). Os tipos A e B apresentam grande identidade em seus domínios maduros, porém nenhuma similaridade é observada na região amino-terminal do peptídeo de trânsito. Em um experimento de importação em cloroplastos isolados, Fts
Publicado em: 2011
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3. Análise de similaridade entre sequências dos genes do HIV-1 e o Genoma Humano / Analysis of similarity between the sequences of genes of HIV-1 and Human Genome
Os genes virais podem ter origens celulares ou não. Eles podem ter sido reunidos durante a evolução, sendo que cada um pode corresponder a uma origem distinta. A similaridade entre sequências genéticas sugerem homologia e, portanto, um parentesco evolutivo. O presente estudo tem como objetivo identificar regiões de similaridade, com suporte estatístic
Publicado em: 2010
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4. Clonagem, caracterização da expressão gênica e do transporte intra-organelar da protease FtsH-p1 de tomate (Lycopersicon esculentum Mill. cv. MicroTom). / Molecular cloning, characterization of the gene expression and intra-organellar transport of tomato (Lycopersicon esculentum Mill. cv. Microtom).
A protease FtsH pertence à superfamília das proteínas AAA (ATPases Associadas à diversas Atividades celulares) cujos membros estão amplamente distribuídos entre procariotos e eucariotos. Nas plantas superiores, elas são codificadas por genes nucleares e sintetizadas por ribossomos citosólicos com uma seqüência de direcionamento na extremidade amino
Publicado em: 2003
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5. Tissue-specific DNaseI hypersensitive sites in the 5'-flanking sequences of the tryptophan oxygenase and the tyrosine aminotransferase genes.
The genes for tryptophan oxygenase (TO) and tyrosine aminotransferase (TAT) are expressed in a tissue- and development-specific manner and are regulated by glucocorticoids (TO and TAT) and glucagon or its intracellular mediator cAMP (TAT) in rat liver. We have analyzed the chromatin structure of these genes in the vicinity of the 5' ends with regard to DNase
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6. HIV-1 Tat binds TAFII250 and represses TAFII250-dependent transcription of major histocompatibility class I genes
HIV Tat, a transactivator of viral transcription, represses transcription of major histocompatibility (MHC) class I genes. Repression depends exclusively on the C-terminal domain of Tat, although the mechanism of this repression has not been known. We now show that repression results from the interaction of Tat with the TAFII250 component of the general tran
National Academy of Sciences.
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7. Two FK506 resistance-conferring genes in Saccharomyces cerevisiae, TAT1 and TAT2, encode amino acid permeases mediating tyrosine and tryptophan uptake.
The macrocyclic lactone FK506 exerts immunosuppressive effects on T lymphocytes by interfering with signal transduction leading to T-cell activation and also inhibits the growth of eukaryotic microorganisms, including Saccharomyces cerevisiae. We reported previously that an FK506-sensitive target in S. cerevisiae is required for amino acid import and that ov
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8. Constitutive Expression of Escherichia coli tat Genes Indicates an Important Role for the Twin-Arginine Translocase during Aerobic and Anaerobic Growth
The transcription start sites for the tatABCD and tatE loci, encoding components of the Tat (twin-arginine translocase) protein export pathway, have been identified. Expression studies indicate that the tatABCD and tatE transcription units are expressed constitutively. Translational fusion experiments suggest that TatA is synthesized at a much higher level t
American Society for Microbiology.
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9. The full-length Tat protein is required for TAR-independent, posttranscriptional trans activation of human immunodeficiency virus type 1 env gene expression.
Tat is a protein that dramatically increases the expression of all genes expressed from the human immunodeficiency virus type 1 (HIV-1) long terminal repeat through interaction with a cis-acting target sequence referred to as TAR (for trans-acting responsive region). The tat gene is divided into two coding exons which, when translated, result in the synthesi
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10. Functional Domains of Tat Required for Efficient Human Immunodeficiency Virus Type 1 Reverse Transcription†
Tat expression is required for efficient human immunodeficiency virus type 1 (HIV-1) reverse transcription. In the present study, we generated a series of 293 cell lines that contained a provirus with a tat gene deletion (Δtat). Cell lines that contained Δtat and stably transfected vectors containing either wild-type tat or a number of tat mutants were obt
American Society for Microbiology.
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11. Construction of recombinant murine retroviruses that express the human T-cell leukemia virus type II and human T-cell lymphotropic virus type III trans activator genes.
Recombinant retroviruses containing the trans activator genes of human T-cell leukemia virus (HTLV) type II and human T-cell lymphotropic virus type III were constructed. The trans activator genes tat II and tat III were inserted into the murine retroviral vector pZIPNEOSV(X)1. Recombinant plasmids were transfected into the psi 2 and psi AM packaging cell li
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12. Codon Optimization of the Tat Antigen of Human Immunodeficiency Virus Type 1 Generates Strong Immune Responses in Mice following Genetic Immunization
DNA vaccines have been successful in eliciting potent immune responses in mice. Their efficiency, however, is restricted in larger animals. One reason for the limited performance of the DNA vaccines is the lack of molecular strategies to enhance immune responses. Additionally, genes directly cloned from pathogenic organisms may not be efficiently translated
American Society for Microbiology.