Generation Marginal
Mostrando 25-36 de 36 artigos, teses e dissertações.
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25. Specific inhibition of outgrowth of Bacillus subtilis spores by novobiocin.
Spores of a Bacillus subtilis mutant temperature sensitive in deoxyribonucleic acid (DNA) replication proceeded through outgrowth at the nonpermissive temperature to the same extent as the wild-type parent spores. In contrast, the DNA synthesis inhibitor novobiocin completely prevented spore outgrowth while displaying a marginal effect on logarithmic growth
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26. The AIDS-Like Disease of CD4C/Human Immunodeficiency Virus Transgenic Mice Is Associated with Accumulation of Immature CD11bHi Dendritic Cells
CD4C/human immunodeficiency virus (HIV) transgenic mice develop an AIDS-like disease. We used this model to study the effects of HIV-1 on dendritic cells (DC). We found a progressive decrease in total DC numbers in the lymph nodes, with a significant accumulation of CD11bHi DC. In the thymus, the recovery of transgenic CD8α+ DC had a tendency to be lower. S
American Society for Microbiology.
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27. Experimentally Determined, Two Locus Fitnesses of DROSOPHILA MELANOGASTER Males
A pilot demonstration of experimental fitness estimation utilizing pedigree data from D. melanogaster was described. Fitnesses were obtained for the male segregants of the two "complex loci," spineless–spineless aristapedia and radius incompletus–inturned. A good fit between selection model and data was obtained, perhaps because the experimental design l
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28. Gene Therapy Applications to Cancer Treatment
Over the past ten years significant advances have been made in the fields of gene therapy and tumour immunology, such that there now exists a considerable body of evidence validating the proof in the principle of gene therapy based cancer vaccines. While clinical benefit has so far been marginal, data from preclinical and early clinical trials of gene therap
Hindawi Publishing Corporation.
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29. Focal localization of the NHE-1 isoform of the Na+/H+ antiport: assessment of effects on intracellular pH.
Na+/H+ exchange (antiport) is a major pathway for the regulation of intracellular pH. Antiport activity is stimulated when suspended cells adhere to the substratum. In this report, immunofluorescence was used to study the subcellular localization of the ubiquitous NHE-1 isoform of the antiport. NHE-1 was not distributed homogeneously on the surface of the ce
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30. src homology 2 domain–containing tyrosine phosphatase SHP-1 controls the development of allergic airway inflammation
Th2 cells are generated from naive CD4 T cells upon T cell receptor (TCR) recognition of antigen and IL-4 stimulation and play crucial roles in humoral immunity against infectious microorganisms and the pathogenesis of allergic and autoimmune diseases. A tyrosine phosphatase, SHP-1, that contains src homology 2 (SH2) domains is recognized as a negative regul
American Society for Clinical Investigation.
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31. Ectodomain of Coxsackievirus and Adenovirus Receptor Genetically Fused to Epidermal Growth Factor Mediates Adenovirus Targeting to Epidermal Growth Factor Receptor-Positive Cells
Human adenovirus (Ad) is extensively used for a variety of gene therapy applications. However, the utility of Ad vectors is limited due to the low efficiency of Ad-mediated gene transfer to target cells expressing marginal levels of the Ad fiber receptor. Therefore, the present generation of Ad vectors could potentially be improved by modification of Ad trop
American Society for Microbiology.
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32. Targeting of the chemokine receptor CCR1 suppresses development of acute and chronic cardiac allograft rejection
Although mononuclear cell infiltration is a hallmark of cellular rejection of a vascularized allograft, efforts to inhibit rejection by blocking leukocyte-endothelial cell adhesion have proved largely unsuccessful, perhaps in part because of persistent generation of chemokines within rejecting grafts. We now provide, to our knowledge, the first evidence that
American Society for Clinical Investigation.
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33. Effects of genes exerting growth inhibition and plasmid stability on plasmid maintenance.
Plasmid stabilization mediated by the parA+ and parB+ genes of the R1 plasmid and the ccd+ and sop+ genes of the F plasmid was tested on a mini-R1 plasmid and a pBR322 plasmid derivative. The mini-R1 plasmid is thought to be unstably inherited owing to a low copy number and to random segregation of the plasmid at cell division, whereas cells harboring the pB
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34. Generation of nitric oxide and induction of major histocompatibility complex class II antigen in macrophages from mice lacking the interferon gamma receptor.
Availability of mice with a targeted disruption of the interferon gamma (IFN-gamma) receptor gene (IFN-gamma R0/0 mice) made it possible to examine parameters of macrophage activation in the absence of a functional IFN-gamma receptor. We asked to what extent other cytokines could replace IFN-gamma in the induction of nitric oxide or major histocompatibility
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35. Segment-specific noncoding sequences of the influenza virus genome RNA are involved in the specific competition between defective interfering RNA and its progenitor RNA segment at the virion assembly step.
The generation of influenza A virus defective interfering (DI) particles was studied by using an NS2 mutant which produces, in a single cycle of virus replication, a large amount of DI particles lacking the PA polymerase gene. The decrease in PA gene replication has been shown to occur primarily at the cRNA synthesis step, with preferential amplification of
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36. Entrainment and resonance in glycolysis
We have proposed a comprehensive model for the glycolytic reaction mechanism and have shown the possibility of self-tuning to resonance, with consequent increase in efficiency of energy transduction by separating the model into two subsystems: one for the phosphofructokinase (PFKase; ATP:D-fructose-6-phosphate 1-phosphotransferase, EC 2.7.1.11) reaction and