Gene Silencing
Mostrando 13-24 de 1059 artigos, teses e dissertações.
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13. RNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesions
The aim of this study was to evaluate the use of RNA interference to inhibit herpes simplex virus type-1 replication in vitro. For herpes simplex virus type-1 gene silencing, three different small interfering RNAs (siRNAs) targeting the herpes simplex virus type-1 UL39 gene (sequence si-UL 39-1, si-UL 39-2, and si-UL 39-3) were used, which encode the large s
Braz J Infect Dis. Publicado em: 2014-08
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14. CIAPIN1 gene silencing enhances chemosensitivity in a drug-resistant animal model in vivo
Overexpression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) contributes to multidrug resistance (MDR) in breast cancer. This study aimed to evaluate the potential of CIAPIN1 gene silencing by RNA interference (RNAi) as a treatment for drug-resistant breast cancer and to investigate the effect of CIAPIN1 on the drug resistance of breast cancer in vivo.
Braz J Med Biol Res. Publicado em: 21/03/2014
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15. Placental hydroxymethylation vsmethylation at the imprinting control region 2 on chromosome 11p15.5
In addition to methylated cytosines (5-mCs), hydroxymethylcytosines (5-hmCs) are present in CpG dinucleotide-enriched regions and some transcription regulator binding sites. Unlike methylation, hydroxymethylation does not result in silencing of gene expression, and the most commonly used methods to study methylation, such as techniques based on restriction e
Braz J Med Biol Res. Publicado em: 22/10/2013
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16. A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
Schistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-09
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17. Dmp53, basket and drICE gene knockdown and polyphenol gallic acid increase life span and locomotor activity in a Drosophila Parkinson's disease model
Understanding the mechanism(s) by which dopaminergic (DAergic) neurons are eroded in Parkinson's disease (PD) is critical for effective therapeutic strategies. By using the binary tyrosine hydroxylase (TH)-Gal4/UAS-X RNAi Drosophila melanogaster system, we report that Dmp53, basket and drICE gene knockdown in dopaminergic neurons prolong life span (p < 0.05;
Genet. Mol. Biol.. Publicado em: 2013
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18. Epigenetic therapy in allogeneic hematopoietic stem cell transplantation
DNA methylation and other epigenetic phenomena appear to be relevant in the pathogenesis of several malignant disorders. DNA methyltransferases add methyl groups to cytosine-phosphate-guanine (CpG) islandsleading to gene promoter silencing. The DNA methyltransferases inhibitors azacitidine and decitabine have anti-tumor activity against a broad range of mali
Rev. Bras. Hematol. Hemoter.. Publicado em: 2013
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19. Functional study of gene PAWR/Par-4 (Prostate apoptosis response-4) in normal and cancer breast cells / Estudo funcional do gene PAWR/Par-4 (Prostate apoptosis responde-4) em células de mama normais e tumorais
O câncer de mama é o tumor mais incidente entre as mulheres no mundo. Assim como em outros tumores, a tumorigênese nas mamas é um processo complexo resultante da combinação de fatores genéticos e ambientais que dirigem a transformação das células normais em células malignas. O gene PAWR, conhecido como PAR-4 (Prostatic apoptosis response-4) foi pr
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 18/06/2012
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20. Silenciamento do gene da enzima PIPKII alfa em células de linhagem eritroleucêmica humana (K562) / Silencing of the PIPKII alfa in human erythroleukemia cell line
As fosfatidilinositol-fosfato quinases (PIPKs) pertencem a uma família de enzimas lipídio-quinases que geram vários mensageiros lipídicos, incluindo um importante segundo mensageiro denominado fosfatidilinositol-4,5-bifosfato, que participa da regulação de diversas atividades celulares, como a modulação do citoesqueleto de actina, o transporte de ves
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/01/2012
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21. Analysis of energetically biased transcripts of viruses and transposable elements
RNA interference (RNAi) is a natural endogenous process by which double-stranded RNA molecules trigger potent and specific gene silencing in eukaryotic cells and is characterized by target RNA cleavage. In mammals, small interfering RNAs (siRNAs) are the trigger molecules of choice and constitute a new class of RNA-based antiviral agents. In an efficient RNA
Genet. Mol. Biol.. Publicado em: 13/11/2012
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22. Stable silencing of β-lactoglobulin (BLG) gene by lentivirus-mediated RNAi in goat fetal fibroblasts
β-lactoglobulin (BLG), a dominant allergen in goat milk, is difficult to remove by traditional biochemical methods. Its elimination from goat milk by genetic modification therefore poses a major challenge for modern goat breeders. A shRNA targeting BLG mRNA with high interference efficiency was identified, with which lentiviral vectors were used for mediati
Genetics and Molecular Biology. Publicado em: 05/07/2012
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23. Silencing of the integrin-linked kinase gene suppresses the proliferation, migration and invasion of pancreatic cancer cells (Panc-1)
Integrin-linked kinase (ILK) is an ankyrin repeat-containing serine-threonine protein kinase that is involved in the regulation of integrin-mediated processes such as cancer cell proliferation, migration and invasion. In this study, we examined the effect of a lentivirus-mediated knockdown of ILK on the proliferation, migration and invasion of pancreatic can
Genetics and Molecular Biology. Publicado em: 17/05/2012
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24. Characterization of anti-silencing factor 1 in Leishmania major
Anti-silencing factor 1 (ASF1) is a histone chaperone that contributes to the histone deposition during nucleosome assembly in newly replicated DNA. It is involved in chromatin disassembly, transcription activation and in the cellular response to DNA damage. In Leishmania major the ASF1 gene (LmASF1) is located in chromosome 20 and codes for a protein showin
Mem. Inst. Oswaldo Cruz. Publicado em: 2012-05