Gene Ipac
Mostrando 1-12 de 26 artigos, teses e dissertações.
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1. Clonagem molecular do gene ipaC de Shigella sp e estudo da expressão por Lactococcus lactis / Molecular cloning and expression analysis in Lactococcus lactis of ipaC gene from Shigella sp
Shigellosis is a severe intestinal infection caused by Gram-negative bacteria which belongs to the genus Shigella. It is a pathology with high incidence and it is known that 99% of cases occur in underdevelopment countries. Among these cases, 69% of them affect children under 5 years old. The high incidence of Shigella in underdevelopment countries, is mainl
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/05/2012
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2. Estudo de associação entre nível de atividade física, risco cardiovascular e o polimorfismo do gene da apolipoproteína e em idosos
INTRODUÇÃO: com o envelhecimento, ocorre perda gradual do volume da massa muscular, responsável por quase toda a perda da força nos idosos. Estudos têm mostrado que a atividade física pode diminuir a morbidade por doenças cardiovasculares, acidente vascular cerebral e aumentar a autonomia e independência. OBJETIVO: analisar a associação entre níve
Rev. bras. geriatr. gerontol.. Publicado em: 2011-03
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3. Análise comparativa da transcrição de genes envolvidos na invasão e escape de Escherichia colienteroinvasora e Shigella flexneriem macrófagos J774 / Comparative analysis of the transcription of genes involved in the invasion and escape of enteroinvasora Escherichia coli and Shigella flexneri in J774 macrophages.
Escherichia coli enteroinvasora (EIEC) possuem características bioquímicas e genéticas semelhantes às de Shigella, porém para que ocorra um processo infeccioso são necessárias 102 células de Shigella em relação a 106 células de EIEC. A patogenicidade de Shigella e EIEC se dá pela presença de um plasmídio de virulência denominado pInv, que cont
Publicado em: 2006
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4. Caracterização molecular do gene ipa de Escherichia coli enteroinvasora pela análise de PCR- RFLP
No presente estudo, foi encontrado polimorfismo no gene ipa em cinco sorotipos de EIEC, de nove estudados. Quando enzimas de restrição SalI e HindII foram utilizadas no ensaio de PCR-RFLP, amostras de EIEC apresentaram polimorfismo em ipaB e ipaD. Por outro lado, não foram observados polimorfismos nos genes ipaA e ipaC nestas cepas, quando diversas enzima
Brazilian Journal of Microbiology. Publicado em: 2004-06
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5. Functional analysis of the Shigella flexneri IpaC invasin by insertional mutagenesis.
The ability of Shigella to enter epithelial cells, to escape from the phagocytic vacuole, and to induce apoptosis in macrophages requires the IpaB, IpaC, and IpaD proteins. An extracellular complex containing IpaB and IpaC can promote the uptake of inert particles by epithelial cells. To determine whether the function of IpaC is to act as an extracellular ch
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6. Identification and characterization of B-cell epitopes of IpaC, an invasion-associated protein of Shigella flexneri.
Invasion plasmid antigen C (IpaC) is a 43-kDa plasmid-encoded protein associated with the ability of shigellae to invade epithelial cells. This protein is consistently strongly recognized by sera from convalescent patients and monkeys experimentally infected with shigellae. The strong immunogenicity of IpaC in the course of natural infection makes it a good
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7. Use of Shigella flexneri ipaC and ipaH Gene Sequences for the General Identification of Shigella spp. and Enteroinvasive Escherichia coli
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8. Use of Shigella flexneri ipaC and ipaH gene sequences for the general identification of Shigella spp. and enteroinvasive Escherichia coli.
The products of the ipaB, ipaC, and ipaD genes are involved in the expression of the invasive phenotype in all species of Shigella and enteroinvasive Escherichia coli (EIEC). DNA probes derived from these genes are accurate indicators of the invasive phenotype (M. Venkatesan, J. M. Buysse, E. V. Vandendries, and D. J. Kopecko, J. Clin. Microbiol. 26:261-266,
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9. Nucleotide sequence and transcriptional regulation of a positive regulatory gene of Shigella dysenteriae.
A 1,937 bp PstI-HindIII fragment containing the ipaR locus was cloned from the large invasion plasmid of Shigella dysenteriae CG097, and its nucleotide sequence was completely determined. The IpaR protein (35 kDa, calculated from the DNA sequence) was synthesized in Escherichia coli chi 1411 minicells containing the 1,937-bp PstI-HindIII fragment. To determi
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10. Surface presentation of Shigella flexneri invasion plasmid antigens requires the products of the spa locus.
An avirulent, invasion plasmid insertion mutant of Shigella flexneri 5 (pHS1059) was restored to the virulence phenotype by transformation with a partial HindIII library of the wild-type invasion plasmid constructed in pBR322. Western immunoblot analysis of pHS1059 whole-cell lysates revealed that the synthesis of the invasion plasmid antigens VirG, IpaA, Ip
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11. Homologs of the Shigella IpaB and IpaC invasins are required for Salmonella typhimurium entry into cultured epithelial cells.
Entry into host cells is an essential feature in the pathogenicity of Salmonella spp. The inv locus of Salmonella typhimurium encodes several proteins which are components of a type III protein secretion system required for these organisms to gain access to host cells. We report here the identification of several proteins whose secretion into the culture sup
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12. Two novel virulence loci, mxiA and mxiB, in Shigella flexneri 2a facilitate excretion of invasion plasmid antigens.
A bank of over 4,200 lacZ protein fusions in Shigella flexneri 2a was screened for fusions to temperature-regulated promoters. One mutant, BS260, was completely noninvasive on HeLa cells and mapped to a region on the 220-kb virulence plasmid in which we had previously localized several avirulent temperature-regulated operon fusions (A.E. Hromockyj and A.T. M