Gardnerella Vaginalis
Mostrando 25-36 de 135 artigos, teses e dissertações.
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25. Comparison of culture and microscopy in the diagnosis of Gardnerella vaginalis infection.
A comparison was made between human blood agar containing amphotericin B, nalidixic acid and either gentamicin or colistin for the isolation of Gardnerella vaginalis from cases of non-specific vaginitis seen in a clinic for sexually transmitted diseases. The medium containing gentamicin was more inhibitory for non-Gardnerella species, but not sufficiently in
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26. Male carriage of Gardnerella vaginalis
The prevalence of Gardnerella vaginalis in the urethra of 430 men attending a clinic for sexually transmitted disease was 11·4%; it was significantly higher in heterosexuals (14·5%) than in homosexuals (4·5%). There was no evidence of rectal or subpreputial carriage of G vaginalis, and urethral carriage was not associated with symptoms of urethritis.
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27. Carriage of Gardnerella vaginalis and anaerobes in semen.
Gardnerella vaginalis was isolated from 22 (38%) of 58 semen samples obtained from men attending an infertility clinic. Counts ranged from 1.2 X 10(3) to greater than 10(7) colony forming units (cfu)/ml. There was no association between the isolation of G vaginalis and the sperm count. Twenty (34.4%) samples contained non-sporing anaerobes and nine (15.5%) b
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28. Identification of Gardnerella vaginalis with the API 20 Strep system.
A total of 137 strains of Gardnerella vaginalis were examined by the API 20 Strep system. The system was shown to be reliable when the tests were compared with standard identification methods, and very little confusion occurred with streptococcal profiles; consequently, G. vaginalis has been included in the API 20 Strep data base.
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29. Rapid microbiochemical method for identification of Gardnerella (Haemophilus) vaginalis.
A rapid biochemical method for the identification of Gardnerella vaginalis has been developed. The method is based on the fermentation of starch and raffinose and on the hydrolysis of hippurate. With this new procedure, identification was confirmed for 390 of 396 G. vaginalis isolates within 1 h after their inoculation into the three substrates.
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30. Humoral circulatory immune response to Gardnerella vaginalis.
Strain-specific circulating immunoglobulin G and/or M was detected by enzyme-linked immunosorbent assay and immunofluorescence test by using Formol-treated suspensions of Gardnerella vaginalis from 28 women with overt vaginitis but only three symptom-free subjects among 43 otherwise healthy women found to be colonized by G. vaginalis. Analogous but less stri
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31. Demonstration by electron microscopy of pili on Gardnerella vaginalis.
Eight strains of Gardnerella vaginalis were examined by electron microscopy for the presence of pili. Narrow pili ranging from 3.0 to 7.5 nm in diameter were seen on bacteria from five of the strains studied.
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32. Phagocytosis and killing of Gardnerella vaginalis by human neutrophils.
Gardnerella vaginalis was ingested and killed by neutrophils in the presence of normal human serum. Heat inactivation of the serum inhibited these processes. The opsonisation of some but not all G vaginalis strains was enhanced by immune rabbit serum. Immune serum did not, however, enhance intracellular killing. Blockade of the classical pathway of complemen
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33. In Vitro Susceptibility of Gardnerella vaginalis to High Concentrations of Sulfonamide Compounds
The excipients of triple sulfa vaginal tablets were ineffective against Gardnerella vaginalis in vitro. The three sulfonamides, however, were inhibitory, and minimal inhibitory concentration tests showed many strains to be susceptible to 25,000 μg of sulfacetamide per ml.
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34. Genomic DNA fingerprint analysis of biotype 1 Gardnerella vaginalis from patients with and without bacterial vaginosis.
Of the 20 biotype 1 Gardnerella vaginalis isolates analyzed, 10 from patients with bacterial vaginosis and 10 from patients without bacterial vaginosis, none shared the same DNA fingerprint. However, a 1.18-kb HindIII fragment was common among 18 of the 20 biotype 1 isolates in a restriction fragment length polymorphism analysis with a 7.9-kb G. vaginalis DN
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35. Evaluation of the enhanced rapid identification method for Gardnerella vaginalis.
The enhanced rapid identification method (RIM; Austin Biological Laboratories), a micromethod for the identification of Gardnerella vaginalis, is based on starch and raffinose fermentation and hippurate hydrolysis. We tested 105 clinical isolates of G. vaginalis with both the RIM and standard biochemical tests. The RIM agreed with the standard biochemical me
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36. Characterization of Gardnerella vaginalis and G. vaginalis-like organisms from the reproductive tract of the mare.
Gardnerella vaginalis has been isolated from women with bacterial vaginosis, from the genital tracts of asymptomatic women, and from several other infected body sites in humans. However, until recently, it has not been isolated from any other animal species. Between June 1988 and October 1989, 31 isolates identified as G. vaginalis and 70 isolates identified