Functional Verification
Mostrando 37-48 de 50 artigos, teses e dissertações.
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37. Quantitative prediction of NF-κB DNA– protein interactions
We describe a general method based on principal coordinates analysis to predict the effects of single-nucleotide polymorphisms within regulatory sequences on DNA–protein interactions. We use binding data for the transcription factor NF-κB as a test system. The method incorporates the effects of interactions between base pair positions in the binding site,
The National Academy of Sciences.
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38. Identification and Functional Verification of Archaeal-Type Phosphoenolpyruvate Carboxylase, a Missing Link in Archaeal Central Carbohydrate Metabolism
Despite the fact that phosphoenolpyruvate carboxylase (PEPC) activity has been measured and in some cases even purified from some Archaea, the gene responsible for this activity has not been elucidated. Using sensitive sequence comparison methods, we detected a highly conserved, uncharacterized archaeal gene family that is distantly related to the catalytic
American Society for Microbiology.
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39. H-NS Regulates DNA Repair in Shigella
We report a new role for H-NS in Shigella spp.: suppression of repair of DNA damage after UV irradiation. H-NS-mediated suppression of virulence gene expression is thermoregulated in Shigella, being functional at 30°C and nonfunctional at 37 to 40°C. We find that H-NS-mediated suppression of DNA repair after UV irradiation is also thermoregulated. Thus, Sh
American Society for Microbiology.
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40. Genetic complementation of the Saccharomyces cerevisiae leu2 gene by the Escherichia coli leuB gene.
The leucine operon of Escherichia coli was cloned on a plasmid possessing both E. coli and Saccharomyces cerevisiae replication origins. This plasmid, pEH25, transformed leuA, leuB, and leuD auxotrophs of E. coli to prototrophy; it also transformed leu2 auxotrophs of S. cerevisiae to prototrophy. beta-Isopropylmalate dehydrogenase was encoded by the leuB gen
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41. Molecular cloning of a 10-deacetylbaccatin III-10-O-acetyl transferase cDNA from Taxus and functional expression in Escherichia coli
The cDNA clone for a 10-deacetylbaccatin III-10-O-acetyl transferase, which catalyzes formation of the last diterpene intermediate in the Taxol biosynthetic pathway, has been isolated from Taxus cuspidata. By using consensus sequences from an assembly of transacylases of plant origin and from many deduced proteins of unknown function, a homology-based
The National Academy of Sciences.
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42. External validation of a six simple variable model of stroke outcome and verification in hyper‐acute stroke
We aimed to validate a previously described six simple variable (SSV) model that was developed from acute and sub‐acute stroke patients in our population that included hyper‐acute stroke patients. A Stroke Outcome Study enrolled patients from 2001 to 2002. Functional status was assessed at 6 months using the modified Rankin Scale (mRS). SSV model perfo
BMJ Group.
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43. Molecular Cloning of α-Amylase Genes from DROSOPHILA MELANOGASTER. II. Clone Organization and Verification
Restriction maps of an α-amylase structural gene clone, λDm65, and of four putative α-amylase pseudogene clones are presented. Two α-amylase structural genes, inverted with respect to each other, are contained in λDm65. Subregions of internal DNA sequence homology within λDm65 and of cross-homology between the presumptive pseudogene clones and λDm65 w
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44. ampG is essential for high-level expression of AmpC beta-lactamase in Enterobacter cloacae.
Mutants of Enterobacter cloacae 55 were studied to delineate more completely the genetics of inducible expression of AmpC beta-lactamase. E. cloacae 55M-L, derived by mutagenesis from a mutant with high-level cefotaxime resistance (MIC, greater than 64 micrograms/ml), E. cloacae 55M, demonstrated a novel phenotype by producing only low levels of AmpC constit
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45. NotI flanking sequences: a tool for gene discovery and verification of the human genome
A set of 22 551 unique human NotI flanking sequences (16.2 Mb) was generated. More than 40% of the set had regions with significant similarity to known proteins and expressed sequences. The data demonstrate that regions flanking NotI sites are less likely to form nucleosomes efficiently and resemble promoter regions. The draft human genome sequence contained
Oxford University Press.
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46. Stimulation of nuclear import by simian virus 40-transformed cell extracts is dependent on protein kinase activity.
We previously reported that both the nuclear import rate of large karyophilic gold particles and the functional size of the pores are significantly greater in simian virus 40-transformed fibroblasts (the SV-T2 cell line) than in nontransformed BALB/c 3T3 cells. In this study, we found that cytosolic fractions obtained from SV-T2 cultures can increase nuclear
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47. Levels of mRNA coding for motoneuron growth-promoting factors are increased in denervated muscle.
Partial denervation of skeletal muscle induces sprouting of axons remaining within the muscle, possibly as a result of increased synthesis by denervated muscle fibers of motoneuron growth-promoting factors. Direct verification of this hypothesis has not been possible because the molecules responsible are not unambiguously characterized. We used Xenopus oocyt
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48. Taxol biosynthesis: Molecular cloning of a benzoyl- CoA:taxane 2α-O-benzoyltransferase cDNA from Taxus and functional expression in Escherichia coli
A cDNA clone encoding a taxane 2α-O-benzoyltransferase has been isolated from Taxus cuspidata. The recombinant enzyme catalyzes the conversion of 2-debenzoyl-7,13-diacetylbaccatin III, a semisynthetic substrate, to 7,13-diacetylbaccatin III, and thus appears to function in a late-stage acylation step of the Taxol biosynthetic pathway. By employing a h
The National Academy of Sciences.