Fluorescent Labels
Mostrando 1-12 de 44 artigos, teses e dissertações.
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1. Use of biotech manipulation of oocytes enclosed in preantral follicles for in vitro evaluation of potential anti-zona pellucida to imunoesterilização bitches. / Utilização da biotécnica de manipulação de oócitos inclusos em folículos pré-antrais para a avaliação in vitro do potencial de anticorpos anti-zona pelúcida para a imunoesterilização de cadelas.
The aim of this study was do evaluate the potential of anti-zona pellucida antibodies for immunosterilization in dogs, through analyzing the capability of anti-porcine zona pellucida (pZP) antibodies to cause atresia of preantral follicles (PF). For this purpose, the biotechnique of manipulation of oocytes enclosed in preantral follicles was employed to esta
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 22/12/2008
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2. Comparison between morphological and staining characteristics of live and dead eggs of Schistosoma mansoni
Schistosoma mansoni eggs are classified, according to morphological characteristics, as follows: viable mature and immature eggs; dead mature and immature eggs, shells and granulomas. The scope of this study was to compare the staining characteristics of different morphological types of eggs in the presence of fluorescent labels and vital dyes, aiming at dif
Memórias do Instituto Oswaldo Cruz. Publicado em: 2006-10
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3. SÃntese e caracterizaÃÃo de nanocristais luminescentes baseados em semicondutores II-VI para fins de aplicaÃÃo como biomarcadores
In this work we present synthetic procedures in aqueous medium for the obtention of luminescent II-VI (CdS/Cd(OH)2 and CdTe/CdS) semiconductor nanocrystals. These methodologies, based on colloidal chemistry, resulted in particles with a size average of 8,5 nm for the CdS/Cd(OH)2 system and 3,8 nm for the CdTe/CdS system. The optical properties of these mater
Publicado em: 2006
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4. Bimane fluorescent labels: labeling of normal human red cells under physiological conditions.
The bimane fluorescent labels, monobromobimane, dibromobimane, and monobromotrimethylammoniobimane, are derivatives of syn-9,10-dioxabimane:1,5-diazabicyclo[3.3.0]octa-3,6-diene-2,8-dione. They efficiently label hemoglobin (reactive thiol groups), membrane proteins, and glutathione of normal human red cells under physiological conditions. Monobromobimane and
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5. Rapid genomic fingerprinting of Lactococcus lactis strains by arbitrarily primed polymerase chain reaction with 32P and fluorescent labels.
Arbitrarily primed polymerase chain reaction, with incorporation of either radioactive or fluorescent labels, was used as a rapid and sensitive method for obtaining genomic fingerprints of strains of Lactococcus lactis. Closely related strains produced almost identical fingerprints. Fingerprints of other strains showed only some similarities.
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6. The preparation of polyamide-oligonucleotide probes containing multiple non-radioactive labels.
Oligonucleotide probes containing multiple non-radioactive labels have been prepared by utilising and extending the methods used to prepare polyamide-oligonucleotide conjugates. The probes were prepared by incorporating suitable amino acid residues, such as lysines, in the polyamide, which were then used as sites for the attachment of the non-radioactive lab
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7. A comparison of non-radioisotopic hybridization assay methods using fluorescent, chemiluminescent and enzyme labeled synthetic oligodeoxyribonucleotide probes.
N4-[N-(6-trifluoroacetylamidocaproyl)-2-aminoethyl]-5'-O-dimethoxy trityl -5-methyl-2'-deoxycytidine-3'-N,N-diisopropyl-methylphosphoramidite++ + has been synthesized. This N4-alkylamino deoxycytidine derivative has been incorporated into oligonucleotide probes during chemical DNA synthesis. Subsequent to deprotection and purification, fluorescent (fluoresce
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8. FLUORESCENT LABELS FOR ANTIBODY PROTEINS. APPLICATION TO BACTERIAL IDENTIFICATION1
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9. Simultaneous Visualization of Peroxisomes and Cytoskeletal Elements Reveals Actin and Not Microtubule-Based Peroxisome Motility in Plants1[w]
Peroxisomes were visualized in living plant cells using a yellow fluorescent protein tagged with a peroxisomal targeting signal consisting of the SKL motif. Simultaneous visualization of peroxisomes and microfilaments/microtubules was accomplished in onion (Allium cepa) epidermal cells transiently expressing the yellow fluorescent protein-peroxi construct, a
American Society of Plant Physiologists.
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10. Site-specific interaction of thrombin and inhibitors observed by fluorescence correlation spectroscopy.
We report on the application of fluorescence correlation spectroscopy (FCS) to observe the interaction between thrombin and thrombin inhibitors. Two site-specific fluorescent labels were used to distinguish between inhibitors directed to the active site, the exosite, or both binding sites of thrombin. For several well-known inhibitors of thrombin, the bindin
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11. New fluorescent cytidine 5'-phosphate derivatives.
Interaction of cytidine 5'-phosphate with chloroacetone or p-tosyloxyacetone leads to 2-methyl-5,6-dihydro-5-oxo-6-(5-0-phospho-beta-D-ribofuranosyl)-imidazo/1,2-c/pyrimidine (2-methylethenocytidine 5'-phosphate) whereas analogous reaction with phenacyl bromide produces similar 2-phenyl-derivative. The bicyclic nucleotides obtained showed significant UV abso
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12. Blind Source Separation Techniques for the Decomposition of Multiply Labeled Fluorescence Images
Methods of blind source separation are used in many contexts to separate composite data sets according to their sources. Multiply labeled fluorescence microscopy images represent such sets, in which the sources are the individual labels. Their distributions are the quantities of interest and have to be extracted from the images. This is often challenging, si
The Biophysical Society.