Fluorescence Anisotropy
Mostrando 1-12 de 153 artigos, teses e dissertações.
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1. Distribution of Xanthene Dyes in DPPC Vesicles: Rationally Accounting for Drug Partitioning Using a Membrane Model
The correct selection of a dye that has effective action as a photosensitizer is a primary concern for successful therapeutic outcomes. The effectiveness of the photodynamic agent is related to both the targeting of cell membranes and the photochemical yield of the chosen dye. The distributions of xanthene derivatives Eosin Y, Erythrosin B, and Rose Bengal B
J. Braz. Chem. Soc.. Publicado em: 2016-11
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2. Integrated experimental biophysics and molecular dynamics simulations of biomolecules in solution - the interaction of nuclear receptors with DNA response elements and the inter-domain dynamics of Cellobiohydrolase I / Estudos por modelagem e dinâmica molecular integradas a técnicas físicas para biomoléculas em solução - interação de receptores nucleares a elementos responsivos no DNA e dinâmica inter-domínios da celobiohidrolase I
Collective motions play a fundamental role in solution biomolecule dynamics and energetics. These movements can couple very distant regions in the protein structures affection, for instance, allosteric mechanisms, the establishment of macromolecular complexes, and on the integrated function of multidomain proteins as molecullar machines. In this thesis, we p
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/09/2011
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3. FOTODEGRADATION OF PHENOTHIAZINES AND THEIR STRUCTURAL EFFECTS ON NA(+)K(+) - ATPASE: A FLUORESCENCE STUDY / FOTODEGRADAÇÃO DE FENOTIAZINAS E SEUS EFEITOS ESTRUTURAIS SOBRE A NA(+), K(+) - ATPASE: ESTUDO ATRAVÉS DE FLUORESCÊNCIA
Chlorpromazine (CPZ), fluphenazine (FPZ) and trifluoperazine (TFP) are phenothiazine derivatives, which generate photoproducts under UV irradiation. We observed that CPZ develops three fluorescent fotoproducts under different conditions. Promazine (PZ) that forms from the CPZ photolysis. The chlorine loss is one of the main pathways for photoproduct formatio
Publicado em: 2010
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4. Photochemistry of conjugated polymers containing charge transfer sites and energy migration / Fotoquímica de polímeros conjugados contendo centros de transferência de carga e migração de energia
Neste trabalho foram estudados os processos de migração e transferência de energia em derivados de polifluoreno, de estrutura totalmente conjugada e de conjugação confinada. A dinâmica de estado excitado dos derivados PF-BNI, PFOPPV e PFPPV foi estudada em solução diluída de diferentes solventes através de espectroscopia eletrônica de alta resolu�
Publicado em: 2010
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5. Estudos moleculares das enzimas Fosfoseril-tRNA sintease de Trypanosoma brucei e Leishmania major e Seril-tRNA sintease de Trypanosoma brucei / Molecular studies the enzymes Fosfoseril-tRNA Kinase of the Trypanosoma brucei and Leishmania major and Seril-tRNA Sintetase of the Trypanosoma brucei
The translation process study is central role in the cellular metabolism and attracts the interest of several groups, in particular, the study of the 21º amino acid, the selenocystein. The selenocystein incorporation pathway was described in Escherichia coli and recently in eukaryotes. The first step of this pathway is initiated by Seryl-tRNA Synthetase tha
Publicado em: 2009
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6. Estudos estruturais do domínio catalítico da proteína tirosina fosfatase eta de rato / Structural studies of the catalytic domain of the rat protein tyrosine phosphatase eta
The rat protein tyrosine phosphatase eta, rPTPeta, is a transmembrane RPTP, with an intracellular portion composed of a unique catalytic region. The rPTPeta and the human homolog DEP-1 are down-regulated in rat and human neoplastic cells, respectively. However, the malignant phenotype is reverted after exogenous reconstitution of rPTPeta, suggesting that its
Publicado em: 2008
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7. Espalhamento de raios-X a baixo ângulo aplicado ao estudo estrutural de proteínas / Small Angle X ray Scattering applied to protein characterization studies
Small angle X-ray scattering has been proven to be a powerful tool in the structural analysis of proteins in solution. This technique permits the three-dimensional visualization of native proteins envelop at the level of nanometers. In this study we discuss the small angle X-ray scattering theory and we proposed a new methodology to determine the molecular w
Publicado em: 2008
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8. Oligomerização, estruturas à baixa resolução, ligação ao DNA e ao ligante dos receptores de hormônios tireoidianos / Thyroid hormone receptor oligomerization, low resolution structures, DNA and ligand binding
The thyroid receptors (TRs) are proteins, which are involved in diverse and important physiological functions in the organisms, since they are regulators of development, cell divison and differentiation, metabolism and homeostasis. They are responsible by the regulation of specific gene transcription, through pleiotropic effects of lipophilic hormones in the
Publicado em: 2008
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9. Técnicas de fluorescência no monitoramento de membranas modelo / Fluorescence techniques to monitor model membranes
Apresentamos os resultados de estudos sobre a utilização de técnicas baseadas no fenômeno de fluorescência para a investigação de processos relacionados a membranas modelo. Nessa investigação, estão envolvidas medidas de propriedades espectrais de absorção e emissão de luz por cromóforos adequados, determinação xperimental de perfis de decaim
Publicado em: 2008
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10. Interação entre proteínas fluorescentes e nanocristais de CdSe/ZnS / Interaction between fluorescent proteins and CdSe/ZnS nanocrystals
Proteins belonging to the GFP family were used to characterize their interaction with fluorescent CdSe/ZnS nanocrystals and to verify the occurrence of resonance energy transfer (FRET) among these elements. Two donor-acceptor pairs were established, one having a protein as donor and the other having a nanocrystal as donor. In both cases the donor suffers que
Publicado em: 2007
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11. First results from the Pierre Auger Observatory
We review in these notes the status of the construction of the Pierre Auger Observatory and present the first Physics results, based on the data collected during the first year and a half of operation. These results are preliminary, once the work to understand the systematics of the detectors are still underway. We discuss the cosmic ray spectrum above 3 EeV
Brazilian Journal of Physics. Publicado em: 2006-12
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12. Time resolved fluorescence anisotropy of basic dyes bound to poly(methacrylic acid) in solution
Soluções de poli(ácido metacrílico) atático, PAMA com peso molecular na faixa de (1,6 a 3,4) x 10(5) g mol-1, e com corantes fluorescentes 9-aminoacridina ou azul do nilo ligados à cadeia, foram estudados por medidas fotofísicas em função da viscosidade e polaridade do solvente. O comportamento dos segmentos da cadeia do PAMA nas vizinhanças da son
Journal of the Brazilian Chemical Society. Publicado em: 2003-10