Flagellin
Mostrando 1-12 de 544 artigos, teses e dissertações.
-
1. Molecular characterization of virulence genes cctA, nanA, and fliC in Clostridium chauvoei from Rio Grande do Sul and São Paulo State, Brazil
RESUMO: Toxina A de Clostridium chauvoei (CctA), neuraminidase (NanA) e flagelina (FliC) são proteínas que contribuem para a patogenicidade de Clostridium chauvoei, o agente causador do carbúnculo sintomático em bovinos. O objetivo deste estudo foi analisar a variabilidade genética dos genes cctA, nanA, e fliC em C. chauvoei isolados em diferentes per�
Cienc. Rural. Publicado em: 02/05/2019
-
2. Production of recombinant flagellin to develop ELISA-based detection of Salmonella Enteritidis
ABSTRACT Food-borne diseases, caused by the pathogenic bacteria, are highly prevalent in the world. Salmonella is one of the most important bacterial genera responsible for this. Salmonella Enteritidis (SE) is one of the non-typhoid Salmonellae that can be transmitted to human from poultry products, water, and contaminated food. In recent years, new and rapi
Braz. J. Microbiol.. Publicado em: 2017-12
-
3. Genetic diversity of Toll-like receptor 5 among pig populations
The Toll-like receptor 5 (TLR5) recognizes flagellin of Gram-positive and -negative bacteria and plays an important role in the host defense system. Here, we surveyed single nucleotide polymorphisms (SNPs) in the coding sequence of the porcine TLR5 gene in 83 individuals from five pig breeds, these including Chinese local populations and Western commercial p
Genet. Mol. Biol.. Publicado em: 18/01/2013
-
4. Influência do reconhecimento da flagelina extra e intracelular no processo de piroptose em macrófagos. / Influence of extra and intracellular flagellin recognition in the regulation of macrophage pyroptosis.
Flagellin is recognized by TLR5, and NLRs NLRC4/Naip5. The latter induce activation of caspase-1 and release of IL-1b and IL-18, besides the death of macrophages by pyroptosis. To investigate the molecular mechanisms involved in these processes, peritoneal MOs were stimulated with flagellin from B. subtilis and S. typhimurium in its free form (activating TLR
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 19/05/2011
-
5. Production of native flagellin from Salmonella Typhimurium in a bioreactor and purification by tangential ultrafiltration
Flagellin is the structural protein and most abundant component of bacterial flagella. The flagellum filament contains around 20,000 - 100,000 subunits of 50 kDa flagellin that can have diverse biotechnological applications such as vaccine adjuvant and cellular protector during chemo- and radiotherapy. The main aim of this work was to study a production proc
Brazilian Journal of Chemical Engineering. Publicado em: 2011-12
-
6. TLR5-dependent immunogenicity of a recombinant fusion protein containing an immunodominant epitope of malarial circumsporozoite protein and the FliC flagellin of Salmonella Typhimurium
Recently, we described the improved immunogenicity of new malaria vaccine candidates based on the expression of fusion proteins containing immunodominant epitopes of merozoites and Salmonella enterica serovar Typhimurium flagellin (FliC) protein as an innate immune agonist. Here, we tested whether a similar strategy, based on an immunodominant B-cell epitope
Memórias do Instituto Oswaldo Cruz. Publicado em: 2011-08
-
7. Identification of putative new Escherichia coli flagellar antigens from human origin using serology, PCR-RFlP and DNA sequencing methods
Escherichia coli has been isolated frequently, showing flagellar antigens that are not recognized by any of the 53 antisera, provided by the most important reference center of E. coli, The International Escherichia and Klebsiella Center (WHO) of the Statens Serum Institute, Copenhagen, Denmark. The objective of this study was to characterize flagellar antige
Brazilian Journal of Infectious Diseases. Publicado em: 2011-04
-
8. Expression of fljB: z66 on a linear plasmid of Salmonella enterica serovar typhi is dependent on FliA and FlhDC and regulated by OmpR
Salmonella enterica serovar Typhi z66-positive strains have two different flagellin genes, fliC:d/j and fljB:z66, located on the chromosome and on a linear plasmid, respectively. To investigate the mechanism underlying the expressional regulation of fljB:z66, gene deletion mutants of the regulators FliA, FlhDC, and OmpR were constructed in this study. The ex
Brazilian Journal of Microbiology. Publicado em: 2010-10
-
9. Human monocytes tolerant to LPS retain the ability to phagocytose bacteria and generate reactive oxygen species
Tolerance to lipopolysaccharide (LPS) occurs when animals or cells exposed to LPS become hyporesponsive to a subsequent challenge with LPS. This mechanism is believed to be involved in the down-regulation of cellular responses observed in septic patients. The aim of this investigation was to evaluate LPS-induced monocyte tolerance of healthy volunteers using
Brazilian Journal of Medical and Biological Research. Publicado em: 2010-09
-
10. Caspase-1 activation and pore formation in murine macrophages infected with Legionella pneumophila / Ativação de caspase-1 e formação de poros em macrófagos infectados por Legionella pneumophila
Legionella pneumophila, the etiological agent of Legionnaires disease, is known to trigger pore formation in bone marrow-derived macrophages (BMMs) by mechanisms dependent on the type IVB secretion system known as Dot/Icm. Here, we used several mutants of L. pneumophila in combination with knockout mice to assess the host and bacterial factors involved in po
Publicado em: 2010
-
11. Molecular differentiation of Salmonella Gallinarum and Salmonella Pullorum by RFLP of fliC gene from Brazilian isolates
Although Salmonella Pullorum and Salmonella Gallinarum cause different diseases in poultry, they are very similar. Both are non-motile and present the same somatic antigenic structure. They are differentiated by biochemical tests. Certain atypical strains are very difficult to distinguish. They do not produce the expected results when dulcitol and ornithine
Revista Brasileira de Ciência Avícola. Publicado em: 2009-12
-
12. Serology of flagellar antigens from strains of enteropathogenic Escherichia coli (EPEC) and Shiga Toxin producing E. coli (STEC) isolated from different animals and comparative analysis of the fliC gene by PCR-RFLP. / Sorologia de antígenos flagelares de amostras de Escherichia coli Enteropatogênicas (EPEC) e E. coli produtoras da Toxina de Shiga (STEC) isoladas de diferentes animais e análise comparativa do gene fliC por PCR-RFLP.
The Escherichia coli species consists of a group of typically non-pathogenic bacteria present in the intestinal tract of humans and animals. Strains are serotyped according to their O (somatic), H (flagellar) and K (capsular) surface antigens, in order to distinguish these microorganisms from the non-pathogenic members of the intestinal microbiota. The flage
Publicado em: 2009