Fimbrial Proteins
Mostrando 1-12 de 117 artigos, teses e dissertações.
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1. Expression, purification and characterization of adhesins involved in biofilm formation Xylella fastidiosa / Expressão, caracterização e purificação de adesinas envolvidas na formação do biofilme de Xylella fastidiosa
É inquestionável a importância da participação da citricultura na economia brasileira. O Brasil é o maior exportador de suco concentrado do mundo. Em 2007 as exportações brasileiras quase alcançaram 400 milhões de caixas de laranja, retrato de uma cultura que gera uma diversidade enorme de empregos diretos e indiretos, movimentando também a indús
Publicado em: 2008
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2. Regulação da expressão da fímbria CupD por sistemas de dois componentes de Pseudomonas aeruginosa Pa14 e ensaios de virulência no hospedeiro-modelo Dictyostelium discoideum / Genes involved with Pseudomonas aeruginosa PA14 pathogenicity: characterization of the promoter regions and virulence assays in the Dictyostelium discoideum host model
Pseudomonas aeruginosa is a ubiquitous gammaproteobacteria able to infect immunocompromised individuals and to cause nosocomial infections. Between two direct repeats in the PAPI-1 pathogenicity island present in strain PA14, there are two gene clusters transcribed in opposite directions. The first, composed of pvrS, pvrR, rcsC and rcsB, encodes two-componen
Publicado em: 2008
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3. Effect of PvrR and RcsB response regulators in motility, biofilm formation and their connection with Pseudomonas aeruginosa PA14 CupD fimbria / Efeito dos reguladores de resposta PvrR e RcsB na motilidade, formação de biofilme e sua relação com a fímbria CupD de Pseudomonas aeruginosa PA14
Pseudomonas aeruginosa is a γ-proteobacteria that can behave as an opportunistic pathogen. The strain PA14 carries two pathogenicity islands, the largest of them, PAPI-1, contains two gene clusters between two direct repeat sequences that are transcribed in opposite directions and are involved in virulence. The first group consists of four genes arrange
Publicado em: 2008
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4. Caracterização eletretroforetica e expressão das fimbrias Fy e 31A de amostrasde Escherichia coli de origem bovina
Escherichia coli strains associated with diarrhea and septicemic infections of cattle were studied to determine the genetic expression of FY and 31A fimbriae. The strains 31A+: BZ43, BZ2468 and 31A, and FY+: Att25, 54-5, 2147-4 and 11a, were evaluated for enterotoxin production (STI and LT), VT, hemolysin, antibiotic resistance, mannose resistant RBC hemaggl
Publicado em: 1990
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5. Antigenic analysis of fimbrial proteins from Moraxella bovis.
Fimbrial proteins were extracted from 15 isolates of Moraxella bovis, and antisera to each of the preparations were raised in rabbits. The antigenic relationships of the fimbriae were investigated by an enzyme-linked immunosorbent assay, tandem crossed immunoelectrophoresis, and a slide agglutination test. With all three methods there was a similar pattern o
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6. Identification and characterization of the genes encoding the type 3 and type 1 fimbrial adhesins of Klebsiella pneumoniae.
Strains of Klebsiella pneumoniae are known to express two morphologically and functionally distinct filaments, the type 3 and the type 1 fimbriae. The gene (mrkD) encoding the adhesion of K. pneumoniae type 3 fimbriae was identified by transcomplementation analysis with the pap fimbrial gene cluster of Escherichia coli. The nucleotide sequence of the mrkD ge
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7. Identification of minor fimbrial subunits involved in biosynthesis of K88 fimbriae.
The nucleotide sequences of the genes faeF, faeH, faeI, and faeJ encoding K88 minor fimbrial subunits were determined. Analysis of the primary structure of the gene products revealed that all four proteins are synthesized with an amino-terminal signal sequence. The molecular masses of the mature FaeF, FaeH, FaeI, and FaeJ proteins were calculated to be 15,16
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8. Identification of major and minor chaperone proteins involved in the export of 987P fimbriae.
The 987P fimbriae of Escherichia coli consist mainly of the major subunit, FasA, and two minor subunits, FasF and FasG. In addition to the previously characterized outer membrane or usher protein FasD, the FasB, FasC, and FasE proteins are required for fimbriation. To better understand the roles of these minor proteins, their genes were sequenced and the pre
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9. Comparison of the genetic determinant coding for the S-fimbrial adhesin (sfa) of Escherichia coli to other chromosomally encoded fimbrial determinants.
DNA probes specific for different regions of the S-fimbrial adhesin (sfa) determinant were constructed and hybridized with DNA sequences coding for P (F8 and F13), mannose-sensitive hemagglutinating type 1 (F1A), and F1C fimbriae. While the sfa and F1C DNA determinants exhibited homology along their entire lengths, the P-fimbrial and type 1-fimbrial determin
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10. Nucleotide sequences of the genes encoding type 1 fimbrial subunits of Klebsiella pneumoniae and Salmonella typhimurium.
The nucleotide sequences of the genes encoding the subunits of Klebsiella pneumoniae and Salmonella typhimurium type 1 fimbriae were determined. Comparison of the predicted amino acid sequences of the two subunits revealed domains in which the sequences were highly conserved. Both gene products possessed signal peptides, a fact consistent with the transport
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11. A minor 987P protein different from the structural fimbrial subunit is the adhesin.
The 987P fimbriae produced by enterotoxigenic strains of Escherichia coli isolated from piglets mediate bacterial attachment to intestinal epithelial cells. These fimbriae consist essentially of a tight helical arrangement of one structural protein subunit encoded by fasA. Fimbriation and specific adhesion requires the expression of seven additional genes (f
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12. Polyclonal anti-idiotypic antibodies exhibit antigenic mimicry of limited type 1 fimbrial proteins of Escherichia coli.
Polyclonal anti-idiotypic antibodies (anti-Ids)(fim) developed against idiotypes on antibodies (Ab-1s) that specifically bind structural, organelle fimbrial proteins of Escherichia coli were able to modulate immune function in anti-Id(fim)-immunized mice. Proliferation or suppression of splenic lymphoid cell responses by polyclonal anti-Ids in tissue culture